heteroduplex

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

... vivo through processes such asreplication errors, heteroduplex formation, deamination of 5-methylcytosine andthe presence of DNA adducts. 1 Cells cope with mismatches by enlistingspecialized proteins that can recognize, excise and correct mismatched bases.This process is known as DNA mismatch repair. In additio...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

...ng characteristics of homoduplex DNA versus heteroduplex DNA. As heteroduplex DNA migrates through the denaturant gradient the areas of non-homology melt at a lower...

DGGE Analysis Using the DCode System, Rev B

...samples in lanes 13 were resolved into two heteroduplex and two homoduplex bands. The heteroduplex bands (upper bands) migrated slower than the corresponding homoduplex bands (lower bands). ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... C and reannealed for 1 hour at 55 C to allow heteroduplex formation. Melting temperature of the 430 b...as a positive control, and demonstrates the heteroduplex bands formed by polymorphic and wild type cDNA. The myeloma cell line 8226, which serves ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

...emperature gradient gel electrophoresis (TGGE), 4 heteroduplex analysis (HA), 5 protein truncation test ...mproved the resolution of the homoduplex bands and heteroduplex bands, thus making it easier to identify mutants from wild-type samples. ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

.... Secondly, the deliberate construction of heteroduplex molecules by pooling homoduplex PCR products often yields unique heteroduplex electrophoresis patterns, enabling identification of different haplotypes that cannot ...

Detection of Mutations in the CYP21 Gene Using the DCode System

...ature for gradual renaturation, thereby generating heteroduplex molecules. Denaturing Gradient Gel Electrophoresis Denaturing gradient gel electrophoresis was carried out using the DCode system (Bio-Rad). Fifteen l of PCR product was loaded on a gel containing a polyacryl...

Heteroduplex Analysis Using the DCode System

... in double-stranded DNA caused by the formation of heteroduplex molecules. 1 Heteroduplex molecules have ...enhance the ability to detect mutations in heteroduplex samples when compared to conventional polyacrylamide gels. 2 In this experiment, we ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...form slow cooling to 40 C. Without this last step, heteroduplex DNA may not be visible on the DGGE gels. In the case of apoA-I, a 420 bp region at the 5' end of the gene, containing the core promoter and the first exon, was amplified using two GC clamped primers such that the final ...

Denaturing HPLC (DHPLC)

...mutations and polymorphisms based on detection of heteroduplex formation between mismatched nucleotides in double-stranded PCR amplified DNA. Sequence variation creates a mixed population of heteroduplexes and homoduplexes during reannealing of wild-type and mutant DNA. When this mixed population is analysed...