glycoprotein

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

... fundamental study of glycoproteins. Typically, a glycoprotein is enzymatically digested, the resulting pep...tures. In this case, as is the case for most glycoprotein analyses, MS/MS is simply not enough. Experimental Conditions Th...

Tools for Proteomic Science II

...nts of carbohydrateand their structures. Using our glycoprotein analysissystem you can determine whether or not your protein is glycosylated, whether the sugar moiety is N- orO-linked, as well as gather additional data regarding carbohydratestructures. Quantification of the sugar moietyand simultaneous staining o...

Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes

...n coronary arteries, and ofinterleukin 6 [IL6] and glycoprotein gp130 in failinghuman hearts [3]). The protocol can be combined withimmunohistochemistry [4, 5], and can also be used forparaffin- and methacrylate-embedded sections. Materials and Methods Preparation of D...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

...combinant baculovirus-expressed TGEV spike glycoprotein vaccines, Am. J. Vet. Res., 58 , 242250 (1997). 6. Stott, D. I., Immunoblotting and dot blotting, J. Immunol. Methods, 119 , 153187 (1989). 7. Wesley, R. D., et al., Differentiation between transm...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

...not found in human milk. 5, 6, 7, 8 βLG is a glycoprotein which exists at the normal pH of bovine milk as a dimer with a molecular weight of 36,000, and consists of two monomeric subunits with molecular weight of 18,000 (162 residues). Several genetic variants of βLG have ...

The Bio-Rad Silver Stain, Rev E

...ion and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor, Cell 40, 191198 (1985) 72. Rosenblum BB et al., Two-dimensional electrophoretic analysis of erythrocyte membranes, Clin Chem 28, 925931 (1982) 73. Simonds WF et al., Pu...

Protein Depletion for Plasma and Serum Proteomic Analysis

... C: Albumin depletion method of improved plasma glycoprotein analysis by two-dimensional difference gel electrophoresis. BioTechniques 2003, 35 :1128-1132. </LI> <LI >Tirumalai RS, Chan KC, Prieto DA, Isaaq HJ, Conrads T, Veenstra TD: Characterization of the low molecular w...