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New Microarray Labeling Kit for Preparing cDNA Probes
...microrrays, which contain hundreds or thousands of genes in asingle format, are increasingly being used to ... inthe levels of hybridization signals of specific genes on the array correlatewith differences in the abundance of those genes in the test and control mRNAu...A New C-Terminal GST Vector for Protein Production in S. pombe
...nase. Expression and purification of heterologous genes often involve fusion of thegene of interest with a purification tag at either the N-terminus or C-terminus.The Schistosoma japonicum GST gene product has been popularly used as aprotein fusion tag for affinity purification of GST fusion proteins. 1 S...Human Primary Preadipocytes and Differentiated Adipocytes
... these cells are useful for analyzing compounds or genes that induce, or are involved with, this important function of the mature adipocyte. Figure 4 Conclusions With Stratagenes new Human Predifferentiated Adipocytes and HumanDifferentiated Adipocyt...New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
...tratagenes mammalian two-hybrid assay kit, hybrid genes are used to detect protein-protein interactions vi...lucidating the in vivo function of newlydiscovered genes and, in turn, will aid in further understanding known proteinfunctions. One of the most powerful me...Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
...y techniques and cell biology research,hundreds of genes encoding receptors, protein kinases, phosphatases,...and PKA are capable of activating the expressionof genes containing SRE and AP-1 or NF- k B bindingelements in their promoter, while the CRE element found in...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
... representation of high-quality cDNA clones, human genes can be quickly identified and subsequently isolated. Purification of ACF Editing apo-B mRNA involves a site-specific deamination reaction that converts cytidine 6666 to uridine and generates a...New Kit Generates Exceptionally Pure Total RNA for RT-PCR
...ith Stratagenes control RT-PCR primers for genes of varying abundance levels in the mouse. 5,6 These include primers for high-abundance transcripts (mouse elongation factor 1 a) , medium-abundance transcripts ( b 2-microglobulin), and low-abundance transcripts (protein phos......type 5 (Ad5) genome and is deleted for the genes E1 and E3 ( Figure 2 ). The expression cas... cells contain and express the transforming genes of Ad5, including E1, which complements the deleted E1 gene from the pAdEasy-1 vector for as...An Epitope Tagging Vector for Gene Expression in Mammalian Cells
...ls Use tag-specific antibodies to study expressed genes Tanya Hosfield Quinn Lu Stratagene The pCMV-Tag1 vector is an epitope tagging vector designed for geneexpression in mammalian cells. A target gene inserted into the pCMV-Tag1 vectorcan be tagged with the FLAG epitope (N-terminal, C-termina...Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
...r cloning studies indicate that five NMDA receptor genes exist,including NMDAR1 (NR1) and four NR2 subunits (NR2A-D). 3 Whenreconstituted in vitro, the functional properties of neuronal NMDAR appear to beconferred by the combination of an NR1 subunit with any of the four NR2subunits. 4 In addition, there...Big Blue Rodent Cell Cultures for Mutagenesis Studies
...es of the effects ofoncogenes and tumor suppressor genes on mutation frequencies and studies of DNArepair. REFERENCES Kohler, S.W., et al. (1991) Proc. Natl. Acad. Sci. USA 88: 78597962. Dycaico, M.J., et al. (1994) Mutat. Res. 307: 461478. Rogers, B.J., et al. (1995) Mutat. ...Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
...ry based on analysis of differential expression of genes [e.g.Differential Display, 2 or serial analysis o...probe signalsderived from traditional housekeeping genes are evaluated. Unfortunately,transcripts for housekeeping genes are abundant, and suboptimal library...Epitope-Tagging Vectors for Functional Analysis in Yeast
...tors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomycescerevisiae. Each...ssion andepitope-tagging analysis of two different genes in a single yeast cell. Characterizing the structure and function of every eukaryotic gene is anece...Safe Production of High-Titer Retrovirus
...sh stable producer lines. Theantibiotic-resistance genes used in the gag-pol and env vectorsare different f...teins is comaintainedwith the antibiotic-resistant genes by including antibiotics in the media. Replication-Defective Retroviral Gene Transfer Systems ......ygromycin, neomycin, and puromycin drug-resistance genes by Cre-Lox recombination; confirming trans...ion vectors by fusing hrGFP to a series of genes that encode subcellularly localized proteins. In Figure 3, each fluorescent microscope photo...Human Universal cDNA Library Array I
...om a universal cDNA library that represent genes expressed in 29 different human tissues. T...nd unique cloning system that allows human genes to be fully isolated in a short period of time with only two hybridization steps. The...Comparing Fidelity and Performance of Proofreading PCR Enzymes
... monitoring phenotypic changes inmutational target genes (lacI 1 and p53 2 ).These studies showed that Pfu DNA polymerase exhibits the lowestintrinsic error rate of any commercial thermostable DNA polymerase. For example,using a PCR mutation assay based upon the lacIOlacZatarget gene, 1 Pfu DNA polymera...Map and Link Human Genetic Disorders with SSLP Analysis
...trated for Sticklersyndrome, with mutations in two genes that encode type XI collagen, COL11A1 andCOL11A2, and in at least one additional unidentified gene, resulting in Sticklersyndrome. 6,7 To determine if the phenotype in a family with Sticklersyndrome is linked to the COL11A1 or COL11A2 locus (COL2A1 ...Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
...ducers Metastasis-inducing genes In vivo metastasis ...n Differentiation-inducing genes Phenotype: differentiation Ion channels P...RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
... the cDNAsequences of mismatch repair genes. These genes code for specialized proteinsthat participate in c...logues soonfollowed with the identification of the genes for the hMSH2, 8 hMLH1, 9 hPMS1, 10 hPMS2 11 and hGTBP 12 proteins. Somemouse analogs to previou...Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
...of these genes. Thetranscription level of cellular genes reflects the activation status of theinvolved sign...re capable of activating the expression of genes containing SRE and AP-1 or NF-kB binding elements in their promoter, while the CRE element f...Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
... identified several differentially expressed genes in the invasive rim of the tumor relative to the noninvasive tumor core. 4 Conclusions The StrataPrep total RNA microprep kit is ideal for the isolation of RNA from small cell samples harvested by LCM. The method genera...A New Lambda Vector for Mammalian Expression
... results in mid-level expression of cloned genes in mammalian cells. Conclusions Stratagenes Lambda ZAP vectors are designed for simplified construction ofhigh-titer cDNA libraries, combined with the capability for excision andrecircularization of cloned insert DNA from lambda phage. With the sim...Versatile Reporter Vectors for Monitoring Viral Transduction
...d selectable markers or readilydetectable reporter genes from the ViraPort retroviral gene expression syste...ossible could be inserted. Dispensing with marker genes eliminates a direct method for measuring theinfectious titer of the resulting retroviral supernatant...Yeast Protein Production System Features High Yields and One-Step Purification
...ther bovine enterokinase or thrombin. As numerous genes that are involved in transcriptional regulation anddevelopmental control have been cloned and identified, the demand forhomogeneously purified proteins for structural and functional analyses hasincreased. Although this demand can be partially fulfill...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
...ly altered suchthat trans-activation of endogenous genes by the ecdysone receptor, or ofthe ponA-responsive...s activation/transcription of 3-flankingendogenous genes from the promoter within the 3 LTRwill not occur in the SIN vector; and inactivation of the proviral...Fractionating DNA Fragments Generated by Differential Display PCR
... (DDPCR) is a widely used technique foridentifying genes differentially expressed between cell types. Appli... side effects. Thus, agreater understanding of the genes involved in the pathogenesis of allergicdisease is needed to allow more acceptable drugs to be devel...The GeneZoo or the GeneJungle?
..., but it will be quite some time before all of the genes and their exon structures are known. Gene finding ...split groups of contiguous exons into two putative genes when they really belong to one gene. They can also group exons into one gene when they really belong...Constructing Directional cDNA Libraries from Limited Amounts of RNA
... described for each library, and the known genes identified for both libraries coordinate with the expected sequences that we observed to be expressed in liver tissue. Table 1: Breakdown of Sequencing Results by Class Class 1: Sequence is identical to a known human ge...Randomize Gene Sequences with New PCR Mutagenesis Kit
...s studies employing the polymerase and GFP genes (data not shown). GeneMorph Kit Produces a Unique Mutational Spectrum In the GeneMorph kit, low, medium, and high mutation frequencies are achieved using a single set of optimal reaction conditions (Mg...Generate High-Quality, Directional Plasmid cDNA Libraries
... for discovering new genes. Traditionally, genes have been selected for sequence homology; more recently, genes are being discovered by gene function. Both lambda and plasmid vectors have been use...Gene-Specific Primers for NMDA Receptor Subunits
...amplification of rat NMDA receptor subunit genes were electrophoresed on an agarose gel ( f...nes RT-PCR primers for rat and human NMDA receptor genes have been tested using cDNA templates derived from whole brain or cultured hNT cells. These new RT-P...Codon Bias-Adjusted BL21 Derivatives for Protein Expression
...liB and lack the Lon protease andthe ompT protease genes that may cause instability and degradation of ther...ld be assessed individually. Even E. coli contains genes with poor codonusage for efficient translation. AGG arginine ...New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
...t obstacle for efficient expression ofheterologous genes in E. coli hosts. In GC-rich genomes, such as mamm...that encodesextra copies of the argU and proL tRNA genes and is able to rescueexpression of genes restricted by either AGG/ AGA codons or CCC codons. The E....High-Titer Retroviral Vectors for Gene Delivery
...m of choice for the invivo delivery of therapeutic genes in the clinic. We introduce the retroviral vectors pFB and pFB-Neo, which are based on MMLVand contain an extended packaging sequence and splice-site configuration thatincreases viral titer and gene expression from the viral promoter. 5 These vector...Identification of Differentially Expressed Gene Products with the CastAway System*
... and development. The identificationand cloning of genes whose expression is modulated is an important step...n of 16 ng/ml. We sought to detect immediate-early genes whose expression was modulated in response to this treatment. Messenger RNA was isol...In Vivo Signal Transduction Pathway Reporting Systems
New tools to study in vivo functions of new genes and drug candidates Li Xu * Tim Sanchez * ChaoFe...ndard techniques in molecular biology, hundreds of genes encoding receptors,protein kinases, phosphatases and other regulatory proteins have been cloned,and ...RT-PCR Primers for the Study of Apoptosis
...rocess that requires theregulation of a variety of genes that interact with and respond to intracellularand...oducing RT-PCR primer sets that are specific for genes involved in inducing and regulating theapoptotic response. These primers are specific for genes enco...Mammalian and Bacterial Expression in One Vector
...which permitshigh-level expression of heterologous genes in both eukaryotic and prokaryoticsystems. The vec...rts in mammalian cells. Expression of heterologous genes in bacteria isregulated by a hybrid T7/ lacO promoter. The pDual vector was engineeredto carry the l...Mammalian Expression Vector for Efficient Cloning of PCR Fragments
... the pCMV-Script vector with two differentreporter genes and have confirmed high-level expression of the ge.... In order to compare expression levels of genes inserted into the pCMV-Script vector with expression levels in other available vector produc...Mammalian Expression Vectors with Interchangeable Selectable Markers
...fers unparalleled convenience. The drug-resistance genes of these vectors can be exchanged without subcloning. Through site-specific recombination, hygromycin, puromycin, and neomycin functional cassettes are quickly and efficiently inserted into the pExchange core vectors. Insertion is carried out in vitr...Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
...ransgenic rodents with recoverable mutation target genes make use of the well-characterized bacterial lacI gene as a mutational target gene. The lacI gene, part of the l LIZ shuttle vector, is present in approximately 40 copies per chromosome in the mouse and rat. Mutations...New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
...teresting proteins modulate the expression of many genes and elicit a widerange of biological functions, in... present in the promoters of various IFN-inducible genes andmodulate their expression. 3 At this time, six different STATs have been cloned and characteriz...Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
...entcloning, sequencing, and expression testing new genes and allow gene analysisexperiments to begin immediately. The versatile dual-expression vector permitsproteins to be expressed and detected in mammalian cells and proteins to beexpressed in and purified from bacterial cells. In the near future, the h...Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
... probe. RNA transcripts of these three A. thaliana genes and aControl Microarray are available separately. Conclusions The discovery clone collection and discovery-3microarray offer unique and valuable tools for combining gene expressionprofiling with gene discovery. The high number of unknown and unchar...Cis-Reporting System Monitors Intracellular Calcium Mobilization
...T(A)GGAAAAT(A/C). 1,2 This site is present in many genes involved in immune responses, includinginterleukin-2 (IL-2), IL-4, IL-5, granular macrophage colony-stimulating factor(GM-CSF), IFN-g, and IL-13. In many cases, for DNAbinding and transactivation, NFAT proteins cooperate with AP-1, which bindsc-Jun/f...Cis-Reporting System Monitors Intracellular Calcium Mobilization
...T(A)GGAAAAT(A/C). 1,2 This site is present in many genes involved in immune responses, includinginterleukin-2 (IL-2), IL-4, IL-5, granular macrophage colony-stimulating factor(GM-CSF), IFN-g, and IL-13. In many cases, for DNAbinding and transactivation, NFAT proteins cooperate with AP-1, which bindsc-Jun/f...New PCR Primers for Human Diseases
...ed in GenBank. These human disease primers amplify genes fromsamples infected with the following organisms: Human immunodeficiency virus,type 1 (HIV-1), 1,2 hepatitis B (HBV), 3 hepatitis C (HCV)(RT-PCR primers*, use cDNA as template), 4,5 herpes simplex virus,types 1 and 2 (HSV-1, HSV-2), 6-8 human he......omosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host animal. For this reason, mice, rats or other small mammals are used to introduce foreign DNA into oocytes or embryos (blastocysts). ...... Microarray design and production All genes relevant for the differentiation and growth of the...' ends of the mRNAs, which represent these genes sequence-specifically. The specificity of these oligonucleotides was tested with PCR*, South...Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
...tz. 1998. Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV. FEMS Microbiology Letters 167:81-88....cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
...entas). RT-PCR was performed on five genes that exhibit various levels of expression. In order to maximize sensitivity the RT reaction was performed separately from the PCR reaction. In the case of Alpha Tubulin and TNFR1, we were able to use a two-step protocol and combined...Enhancing PCR* Efficiency with MasterTaq
...nalysis, a search is undertaken for target genes that are regulated by certain conditions, eg, appl...ive RT-PCR. As many sequences are low-copy genes and thus difficult to detect on Northern blots, RT-PCR is preferred in these cases. The resu...Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
...ndividual differences in expression of the genes in individual cells. The experiments for coexpression of both genes in the individual cells were carried out using the two-step multiplex RT-PCR strategy (Fig. ...The use of TaqMaster PCR Enhancer
... used to introduce mutations into specific genes in the mouse genome. The transgenic DNA is typically transcribed and translated in a tissue-specific and stage-specific pattern that is determined by regulatory sequences present in the transgenic DNA. Consequently, ...The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
...lymerase for the amplification of 16S rDNA genes from humic substances-contaminated DNA ext... and EUB1492 were used to amplify 16S rDNA genes from the extracted and purified chromosomal DNA.11,12 PCR was performed with a Eppe...The QIAGEN Guide to Animal Cell Culture
...tended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). The phenotype of these cultures is intermediate between finite cultures and continuous cultures. The cells will proliferate for an extended time, but usually the culture will eventually cease d...QIAGEN Instrument Service insist on the best in service and support
...igo Set Version 1.0 for screening large numbers of genes using RNAi techniques The ...gene-silencing studies of 139 human cancer-related genes for functional genomics studies using RNA interference (RNAi). The Cancer si......ase pairs in haploid genome For single-copy genes 2. General guidelin...ction. This allows research on the role of certain genes in complex biological processes. Modified genomic loci can be easily distinguished by mu......irs in haploid genome For single-copy genes 2. Primer design, concentration, and storage Standard PCR primers Prerequisites for successful PCR include the design of optimal primer pairs, the use of appropriate prim...Other ContentsinjuryinjuryinjuryinjuryinjuryinjuryinjuryinjuryinjuryeventeventeventeventeventeventeventeventeventtraumatictraumatictraumatictraumatictraumatictrichinosistrenchtremortremortremortrichomoniasisOther TagsHaeHaeHaeHaeHaeSmaSmaSmaSmaSmaSmaSmaSmaBamHBioVisionRangeRangeRangeRangePLAUMMPOther Tagsrandom 2random 3random 4random 5random 6exploring 2exploring 3exploring 4exploring 5exploring 6secrete 2secrete 3
(Date:10/15/2008)... nanoparticles being tested at the National Instit...chers seeing stars. In a recent paper,* NIST scien...RS) to demonstrate that gold nanostars exhibit opt...l and biological sensing and imaging. These unique...ange of applications from disease diagnostics to c...
(Date:10/15/2008)..., professor of molecular biology at Rockefeller Un...theoretical physics at New York University and at ...lexander von Humboldt professorships ever to be aw...nominated the two scientists for this distinction,...dt Foundation panel. This distinction, which comes...
(Date:10/15/2008)...cher Dr. Fred H. Gage, professor in the Laboratory...ience Prize for his discovery of the physiological...e will officially receive the award during a cerem...an on Nov. 21. , Dr. Gage,s laboratory concentra...cted plasticity and adaptability to environmental ...
(Date:10/15/2008)...(October 28, 2008) -- A study(1) published in the ...ical Sciences, which is published by Oxford Journ...bes the kidney toxicity of melamine and cyanuric a...e the toxicity of the compounds that contaminated ...points to a possible link between the pet food con...
Breaking Biology News(10 mins):Gold nanostars outshine the competition 22 Alexander von Humboldt professorships go to LMU Munich 22 Alexander von Humboldt professorships go to LMU Munich 32 Alexander von Humboldt professorships go to LMU Munich 42 Alexander von Humboldt professorships go to LMU Munich 5Ultrafast optical shutter is switched entirely by laser light 2098 1Ultrafast optical shutter is switched entirely by laser light 2098 2Ultrafast optical shutter is switched entirely by laser light 2098 3Detroit Homeless Men Adopt Local Families for Christmas 7802 1Detroit Homeless Men Adopt Local Families for Christmas 7802 2American Dietetic Association Supports USDAs New WIC Food Packages 7797 1American Dietetic Association Supports USDAs New WIC Food Packages 7797 2Participation in organized high school activities lowers risk of smoking 3 years after graduation 7792 1Participation in organized high school activities lowers risk of smoking 3 years after graduation 7792 2Participation in organized high school activities lowers risk of smoking 3 years after graduation 7792 3(Date:10/15/2008)... Will Educate Parents about Prescription,Drug Abus...mily Partnership (NFP),has designated Monday, Octo...g off the group,s 23rd annual Red Ribbon Week from...ents with action steps to prevent children,s,presc...million teens in the United States abuse,prescript...
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