fixation

Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

...of tissue array technology through optimizedtissue fixation and processing procedures that preserve tissue mor...es. In this product line, optimized tissue fixation preserves morphological integrity, antigenicity of proteins, and nucleic acid reactivity. On...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

...fication and downstream analysis. Depending on the fixation protocol, the age of the samples, the staining procedure, and the storage conditions used, RNA and DNA may be highly fragmented into pieces smaller than 300 nucleotides, thus limiting the size of fragments ...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

...nsthat are expressed on the cell surface.Following fixation and permeabilizationof cells, intracellularantigens can be easily detected byantibody staining. Detection of proteins generallydepends on the availability of asuitable antibody, many of whichare commercially available in afluorescently conjugated for...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

...ing procedure)and air dried for 10 seconds.After a fixation step of 5 minutesin 70% ethanol at -20 C and ashort wash in RNase free water(10 seconds) the sections werefurther processed according tostandard histochemical procedures.Depending on the subsequenttests either Hematoxylin/Eosin(HE), Nuclear Fast Red ...

Optimum FFPE RNA Isolation Kit

...ns can be quite challenging. The fixation process causes cross-linkage between nucleic acids...n occur before completion of the fixation process. The optimal fixation time is 12-24 hours, using neutral-buffered formalin...

Protect RNA and Improve Target Cell Identification

...raffin-embedded tissue--formalin fixation introduces cross-links between nucleic acids and proteins, resulting in RNA fragmentation. However, RNA extraction from frozen tissue is also complicated by reactivation of endogenous RNases that ...

Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

...dicating that other modifications from the fixation process have adversely affected the RNA as a reverse transcription template. Figure 1. Ct Values from Real-Time RT-PCR of Frozen and FFPE Mouse Brain. Half of each of four mouse brain samples were flash froz...

Working With Laser Capture Microdissection Samples

...NA isolation. This is because formalin fixation introduces crosslinks between nucleic acids and proteins, resulting in fragmentation of the RNA. Freezing avoids crosslinking but introduces a different problem. Endogenous nucleases are not irreversibly inactivate...

Your Data: RNAlater Stories

... RNAlater with Kennedy Space Center fixation tubes, allowing safe and effecti... station era: Utilization of KSC fixation tubes with RNA later (2005) Acta Astronautica (56) :623-628. ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...ication without a heat denaturation step (formalin fixation also contributes to DNA denaturation) was completed over 18 h at 30 °C, followed by enzyme inactivation by heating at 65 °C for 10 min. Yield was determined by the PicoGreen dsDNA method. Suitability for use in Phi29 DNA polymerase�med...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

...at room temperature. b. After fixation was completed, thetissues were immediately process...sed, they simply illustrate the effect of formalin fixation on the RNA. These profiles further illustrate the need for a robust method of amplification to overc...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

... over other reporters of not requiring cell/tissue fixation for visualization. EGFP fluoresces green when exci...lubility of EGFP when expressed as a free protein, fixation is required to prevent leaching of the protein and retain fluorescence after cryosectioning. Recentl...

An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis

...designed to work with live cells in order to avoid fixation artifacts such as the disruption of tubules. Methods Human Umbilical Vein Endothelial Cells (BD HUVEC-2, Cat. No. 354151) were maintained in EGM-2 (Cambrex, CC-3156). The cells were washed and trypsinized (trypsin- EDTA, Cambrex, CC-...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...dehydepreservation on DNA. While the conditions of fixation are myriad and the possibleproducts complex 4 , pa...There are limits to all WGA techniques, and tissue fixation by formaldehyde is a highlyvaried procedure that affords extracted DNA of vastly different qualities...