exon in Biological Technology

AMT Accesses Technology for Treatment of Duchenne Muscular Dystrophy

... will start its pre-clinical phase this year." exon skipping in DMD exon skipping is a technology to neutralize genetic ... cannot take place. However, when the faulty exon is eliminated (i.e. skipped), protein synthesis ...

In vivo visualization of alternative splicing

... changes during the lifetime of the worm: Embryos and early larvae express exon 9, while adult worms express exon 10. By linking exons 9 and 10 with differently colored fluorescent ...

First Catalog Cancer Biochip for Sequence Capture Now Available from febit

... febit will stay at the forefront of cancer research and include key research study results in the next generation of febit's cancer biochips. A 2Mb exon cancer biochip will be available this summer and a 30Mb biochip is planned already for release in 2010. Multiplex NGS studies based on ...

Jivan Focuses on Solutions for Transcriptome Sequencing

... Arrays for targeted sequencing of genomes, gene families and therapeutic areas. By using Jivan's database of mRNA sequences, including exons and exon junctions, Jivan can ensure the capture of all mRNA sequences of each gene of interest, including wildtype transcripts and splice variants. For genes ...

ExonHit Launches Rat Genome SpliceArray(TM) Products to Expand High Resolution Gene Expression Profiling for use in Disease Modeling and Toxicogenomics

... transcripts is now offered through our portfolio of genomic services. The Rat SpliceArray(TM) monitors over 23,000 genes utilizing over 200,000 exon body and 180,000 splice junction regions. This comprehensive coverage enables investigators to further elucidate the enormous diversity of the rat ...

Jivan Awarded US Patent #7,340,349 for Splice Array Analysis

... The issued claims cover mathematical algorithms for determining the expression levels of splice isoforms of a gene in a sample using microarrays with exon junction probes. "The '349 Patent validates JIVAN's position as a leader in the growing market for DNA microarrays to detect alternative splicing," ...

Exelixis Reports Encouraging Interim Data From a Phase 2 Trial of XL647 as First-Line Therapy for Non-Small Cell Lung Cancer

... as their best response (8 PR and 11 SD out of 30 evaluable patients). -- Of the 8 patients who experienced partial responses, 4 had EGFR exon 19 deletions, 1 had an EGFR L858R mutation, and 3 were EGFR wild-type. -- All 6 patients with EGFR-activating mutations demonstrated clinical ...

Heteroduplex Analysis Using the DCode System

... exons 7 and 10. The heterozygous mutations from exon 7 were 1154insTC, two base insertion ... deletion (-TTC). The mutations from exon 10 were a heterozygous ΔF508, three base ... to create end products of 289 bp for exon 7 and 369 bp ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... at the following nucleotide positions: exon 2: 2011 (A→C) and exon 5: 2851 (T→G). Nucleotide ... M12792; M23280. The mutant control sample for exon 6 contains the cluster-E6 mutation and ...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

... nM each dNTP, 800 nM each primer (MP 1: exon 1 + exon 4 + exon 13, MP2: ...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

... from breast cancers were examined from exon 5, 6, 7 and 8 of the p53 gene. They were ... mM EDTA). Four different PCR products each from exon 5, 6, 7 and 8 were mixed in a 25 l ... The sample missed showed a mutation of exon 7 on perpendicular gels but not on ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... Non-Polyposis Colon Cancer. We selected exon 4 of the hMSH2 gene as a model system ... by site directed mutagenesis in an exon 4 containing clone. The individual ... SITE-DIRECTED MUTAGENESIS OF hMSH2 exon 4 A series of mutations was designed ...

Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer

... each hybridization several DMD gene exon probes were chosen, both within and outside ... status was known4. For each sample at least 1 exon was chosen within the deleted/duplicated ... exon specific peaks were all normalized against ...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

... Amplification of Epstein-Barr Virus exon C with Difficult Primers Using ... and the 3'-terminal of each exon were synthesized in order that the cloned ... cycler. However, this was not the case for exon C. The properties of primers used for ...

Protein Truncation Tests Using the DCode System

... indicated a deletion of 174 bp, corresponding to the entire exon 26. Because this cDNA mutation would result in an in-frame ... a homozygous G to A substitution at the last nucleotide of exon 26 (nt 3576). This mutation is referred to as 3576 G > A ...

TTGE on the DCode System

... For example, the theoretical melting curve of a GC-clamped exon 2 sequence from the BRCA1 gene is very flat, containing only ... the advantage of TTGE over CDGE and DGGE is shown for the KRAS exon 1 gene in Figure 2AD. Since the denaturing conditions ...

Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE

... from tissue or from fluid obtained from patients at ERCP. K-ras exon 1 was amplified from genomic DNA using a thermal cycler and ... Prism (Perkin Elmer). Results exon 1, containing codons 12 and 13, of the K-ras gene was examined for ...

Get the 5'-End

... heterologous transcription start sites and alternative first exon usage. Finally, the first exon of an mRNA typically includes both a non-coding region ...

Efficient Cellular Fractionation for RNA and Protein Isolation

... (35 cycles), both forms of the alternatively spliced fibronectin exon EIIIb mRNA were detected in total, cytoplasmic, and nuclear ... The mutually exclusive alternative splicing of fibronectin exon EIIIb was analyzed by RT-PCR (35 cycles) using total (T), ...

Mutation detection for the K- rasand P16 genes

... shown to be mutated, sequencing can pinpoint the exact mutation. For P16 exon 3, the PCRs are electrophoresed on the Agilent 2100 bioanalyzer, ... sequence chromatograms for these samples. The analysis of the exon 3 region of the P16 gene was researched. At first, the discovery of ...

QIAGEN Multiplex PCR Handbook

... 2), or together (Exon 2 and 4). The PCR products generated from exon 4 and exon 2 were 395 bp and 242 bp respectively. However, in the multiplex ...

The TripleMaster PCR System for three different amplifications

... -actin gene was amplified. The intron sequence between exon 1 and exon 2 of the human -actin gene has 85 % GC content and contains ...

Efficient and Reliable PCR Setup Using Eppendorf MasterMix

... fragment length and template Lane 1: 160-bp fragment from exon 26 of the human gene of the apolipoprotein B, (genomic DNA) Lane 2: 360-bp fragment from exon 26 of the human gene of the apolipoprotein B, (genomic DNA) ...

Gene-Specific Primers for NMDA Receptor Subunits

... NR1, Rat; flanking C1 exon C1 + , 214 C1 , 103 NR1, Rat: flanking N1 exon N1 + , 235 N1 ,172 ...

SNPs Powerful Tools for Association Studies

... case, the nucleotide change that results in a RFLP is also the causative change (i.e., an A-to-T change in the number two nucleotide of the sixth exon in the - globin chain of the hemoglobin gene, Kan and Dozy, 1978). However, RFLPs only very rarely cause phenotypes and the number of RFLP markers ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... degree of site and size variation than coding regions. Introns may be amplified using conserved primers targeted to flanking exon sequence, a strategy termed exon-primed intron-crossing (EPIC) amplification. 7 When the size of a homologous intron varies among ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... Methods Mutant and wild-type genomic DNA were isolated from cell cultures. The samples were amplified for exon 8 of the p53 gene according to Borresen et al., using a 20-mer primer (5-ATCCTGAGTAGTGGTAATCT- 3) and a 60-mer primer ...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

... Here we describe a method for the separation of HLA-DRB alleles, following PCR amplification of the highly variable second exon of HLA-DRB alleles, using denaturing gradient gel electrophoresis (DGGE). When separation of HLA-DRB alleles is followed by direct ...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

... tissues of tumor free pancreases. We used microdissection followed by a sensitive single step PCR regime to amplify the exon 1 of the K-ras gene. Point mutations were detected by constant denaturing gel electrophoresis (CDGE) 5 using the DCode universal ...

Thermal Cycling Profile for Standard PCR

... Figure 1: Effect of excessive cycling on impure and pure templates A PCR product (245 bp amplicon from exon 6 of the dopamine 2 receptor gene) was reamplified in a series of reactions. In one set of experiments, the template was not ...

The GeneZoo or the GeneJungle?

... new discoveries. The raw sequence of the human genome is now mostly complete, but it will be quite some time before all of the genes and their exon structures are known. Gene finding software programs are inaccurate at spotting exon-intron junctions. They can split groups of contiguous ...

Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

... designed to rapidly detect/screen the most common mutation that causes cystic fibrosis (CF). This mutation, a three-base-pair deletion, within exon 10 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, accounts for approximately 70% of all mutant CF alleles. 3,4 The ...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... quantitative PCR core reagent kit with another commercially available core reagent kit to amplify and detect a 294-bp region in exon 3 of the human b -actin gene using a TaqMan probe (Figure 1). Three concentrations (100, 1, and 0.01 ng) of human genomic DNA ...