Epitope in Biological Technology

ARIUS announces cancer stem cell antibody program successfully completes first toxicology study

... -Lead antibody targeting novel epitope of CD44 found in breast colon, and ... in animal models. ARIUS' program targets a novel epitope of CD44 that has been found in a number of cancer ... demonstrated that its antibody targets a novel epitope of CD44 that is present on the majority of human ...

CEL-SCI Developing Immune-Based Treatment Against Swine and Other H1N1 Flu Viruses Using Proprietary L.E.A.P.S. Technology

... H1N1" flu viruses, utilizing its proprietary L.E.A.P.S.(TM) (Ligand epitope Antigen Presentation System) vaccine technology. The Company plans to ... peptides. The L.E.A.P.S. constructs containing the antigenic disease epitope linked to an Immune/T-cell binding ligand (I/TCBL) can be manufactured by ...

CEL-SCI's L.E.A.P.S. Technology Shows Potential to Treat Swine Influenza in Preclinical Studies

... that vaccines utilizing its proprietary L.E.A.P.S.(TM) (Ligand epitope Antigen Presentation System) vaccine technology could potentially induce ... peptides. The L.E.A.P.S. constructs containing the antigenic disease epitope linked to an Immune / T-cell binding ligand (I/TCBL) can be manufactured ...

CEL-SCI Presents Data at Prestigious Medical Conference Demonstrating That Its L.E.A.P.S. Technology Stimulates Maturation of Human Blood Cells Into Dendritic Cells Suggesting Potential for Prevention and Treatment of Infectious Disease

... which demonstrates how vaccines utilizing its L.E.A.P.S.(TM) (Ligand epitope Antigen Presentation System) vaccine technology can induce protection ... peptides. The L.E.A.P.S. constructs containing the antigenic disease epitope linked to an Immune / T-cell binding ligand (I/TCBL) can be manufactured ...

ExonHit Therapeutics Invested Strongly in 2008 for Pivotal 2009 Results

... - Expanding EHT 202 program to other CNS indications - Initiation of epitope discovery programs for ocular disorders with Allergan - ... a variety of ocular disease related models in order to discover novel epitope targets for the development of therapeutic antibodies. EHT/AGN ...

Genmab Reaches Milestones in Ofatumumab Collaboration

... next generation monoclonal antibody that targets a distinct small loop epitope on the CD20 receptor on the surface of B-cells. This epitope is different to the other anti-CD20 antibodies currently available or in ...

Genmab Announces Details of Planned Ofatumumab Phase II Study in Multiple Sclerosis

... fully human, next generation monoclonal antibody that targets a unique epitope of the CD20 receptor on the surface of B-cells. Other anti-CD20 antibodies currently available or in development bind to a different epitope on the CD20 receptor. Ofatumumab is being developed under a co-development ...

Genmab Announces Update on Recruitment of Patients in ofatumumab CLL Pivotal Study

... fully human, next generation monoclonal antibody that targets a unique epitope of the CD20 receptor on the surface of b-cells. This epitope is different to the other anti-CD20 antibodies currently available or in ...

CEL-SCI Files Patent Application to Support Company's Treatment for More Virulent Strain of H1N1 Swine and Other Influenza Viruses

... mimic cell/cell interactions on the dendritic and T-cell surface with synthetic peptides. The L.E.A.P.S. constructs containing the antigenic disease epitope linked to an Immune/T-cell binding ligand (I/TCBL) can be manufactured by peptide synthesis or by covalently linking the two peptides. Depending ...

PharmAthene Presents Data for SparVax(TM) and Valortim(R), a Novel Vaccine and Anti-Toxin for the Prevention and Treatment of Anthrax Infection at the 7th Annual ASM Biodefense Meeting

... monkeys (cynos) is believed to follow a similar disease course as in humans. Valortim(R), a fully human monoclonal antibody that binds to a distinct epitope on protective antigen, has demonstrated preliminary efficacy in the post-exposure prophylaxis and therapeutic settings in these animal models. ...

Epitomics Appoints Dr. Zhiqiang An as Chief Scientific Officer

... "RabMAb(R) is a novel class of monoclonal antibodies which possess unique biological properties, including ultra-high affinity, diversity in epitope recognition, and high target specificity. Epitomics provides me with the opportunity to expand the healthcare promises of biologics by developing ...

Publication on 'Tregitopes' in Blood Journal of October 2008

... generate new therapeutics for cancer and autoimmune diseases as well as new vaccines for infectious diseases such as HIV, TB, and hepatitis. T cell epitope mapping, the selection of target peptides from any protein sequence, is a powerful resource for the development of novel protein therapeutics. EpiVax ...

Genmab Reaches Milestone in Ofatumumab Collaboration

... in Japan. Ofatumumab is an investigational, new generation, human monoclonal antibody that targets a distinct membrane proximal, small loop epitope ( specific binding site) of the CD20 molecule on the surface of B-cells. Ofatumumab is being developed to treat chronic lymphocytic ...

Circassia Acquires North American and Japanese Rights to Dopexamine

... Lansdowne Partners, Goldman Sachs and Invesco Perpetual. Circassia's lead products target allergies utilizing the company's proprietary T-cell epitope desensitization technology, ToleroMune(R). The company is also developing its ToleroTrans(R) organ anti-rejection technology, which uses a similar ...

Circassia Initiates Phase II Clinical Study With Novel Anti-Allergy Technology

... Lansdowne Partners, Goldman Sachs and Invesco Perpetual. Circassia's lead products target allergies utilizing the company's proprietary T-cell epitope desensitization technology, ToleroMune, which was originally developed at Imperial College London. The company is also developing its ToleroTrans ...

Circassia Raises GBP11 Million ($21.8 Million) in Oversubscribed Second Round Funding

... of world-class venture capital and institutional investors. Circassia's lead products target allergies utilising the company's proprietary T-cell epitope desensitization technology, which was originally developed at Imperial College London. The company is also developing its ToleroTrans organ ...

Arius completes Pre-IND Meeting with FDA for Lead CD44 Cancer Stem Cell Program

... discuss the upcoming IND submission for ARH460-16-2, its lead CD44 Cancer Stem Cell Program. ARH460-16-2 is an anti-cancer antibody targeting a novel epitope of CD44 found in hematological and solid cancers, including breast, colon and prostate. The Pre-IND meeting focused on development plans for the ...

ARIUS announces manufacturing agreement with Avid Bioservices for CD44 Cancer Stem Cell antibody

... ARIUS is advancing the formal pre-clinical toxicology program for its lead CD44 Cancer Stem Cell program, an anti-cancer antibody targeting a novel epitope of CD44 found in breast, colon, and prostate cancers. Pre-clinical data from a dose-ranging pilot toxicology study in non-human primate models ...

Arius Features Preclinical Data on Antibody Candidates at Two Conferences

... which demonstrate that it is a potent therapeutic in breast cancer models both in vivo and in vitro. The poster also highlights the prevalence of the epitope targeted by Arius' antibody in breast cancer patients. The poster presentation is entitled "Development of a potent therapeutic monoclonal antibody ...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

... antibody recognizing an endogenous epitope may be used. Agonist treatment produces an ... HEK293 cell line expressing VSV-G epitope tagged β2-adrenergic receptor HEK293 cell line expressing VSV-G epitope tagged δ-opioid receptor ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

... endogenous antibody that recognizes an exofacial epitope can be labelled directly with CypHer5. ... HEK293 cell line expressing VSV-G epitope tagged β 2-adrenergic receptor

HEK293 cell line expressing VSV-G epitope tagged δ -opioid receptor ...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

... assay system, a CypHer5E-conjugated anti-VSV-G epitope antibody is incubated with a clonal stable HEK 293 cell line expressing N-terminally epitope tagged β2-adrenergic receptor. Upon agonist ...

HEK293 cell line expressing VSV-G epitope tagged β2-adrenergic receptor

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... sequence is provided with each clone. epitope and Purification Tags All clones ... site, three copies of the c-myc epitope tag, and a single copy of the HIS6 purification tag ( Figure 1). The c-myc epitope is derived from the human c-myc gene and contains ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... vectors also contain DNA sequences coding for epitope peptides that can be specifically ... sequence for the FLAG epitope (DYKDDDDK 7 ) is located in the MCS downstream ... of the GAL1 promoter; a sequence for the c-myc epitope (EQKLISEEDL 8 ) is located in the MCS ...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

... An epitope Tagging Vector for Gene Expression in Mammalian ... Stratagene The pCMV-Tag1 vector is an epitope tagging vector designed for gene expression in ... pCMV-Tag1 vector can be tagged with the FLAG epitope (N-terminal, C-terminal or internal ...

A New C-Terminal GST Vector for Protein Production in S. pombe

... results in a protein with the FLAG epitope tag attached on its C-terminus; cleavage by ... proteins that are tagged with the FLAG epitope at N-terminal, C-terminal and internal positions ... As shown in figure 3 , the FLAG epitope contained in the calmodulin-GST fusion protein ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... 1989) or a TGEV neutralizing monoclonal antibody specific for epitope A of the S-protein (25C9) and non-neutralizing monoclonal antibodies specific for epitope D of the S-protein (44C11) and N-protein (25H7) (Lanza et al., ...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

... contiguous copies of either the FLAG or hemagglutin (HA) epitope tag sequence, respectively. In both vectors, duplicate stop codons follow the final epitope tag sequence to ensure termination of the inserted gene transcript ...

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

... specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, ... antibody to each new protein under study. 1 epitope tags are usually short polypeptides with defined ... in samples derived from mammalian epitope tagging. 3 As shown in Figure 3 , we ...

Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays

... total target assays report the level of the protein regardless of its phosphorylation state. Typically, because of epitope binding competition for the same target protein, a total target assay should not be multiplexed with the corresponding ...

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

... to the humoral responses demonstrated in our work to date. The IPQSLDSWWTSL peptide containing the dodecameric class I Ld-restricted epitope of the hepatitis B envelope will be used in cytotoxic T cell assays and tetramer analysis. This not only allows the generation ...

A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage

... can serve as an early marker of apoptosis since it is directed against the caspase cleavage site of PARP-1 and specifically recognizes an epitope that is generated by caspases, but is not accessible in nonapoptotic cells. This easy-to-use addition to the Roche ...

Tools for Proteomic Science II

... proteins. epitope tagging epitope tagging eliminates the laborious and ... conjugated forms, and recognize commonly used epitope sequences, such as the influenza hemagglutinin ...

Uses and Applications of FuGENE 6 Transfection Reagent

... transfection-grade plasmid preparation (e.g., the -gal control vectors supplied with the Mammalian Expression Vectors for epitope Tagging (Cat. No. 1 814 664). Note: Endotoxins are reported to be cytotoxic to some very sensitive cell lines (e.g., ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... has the same features with the addition of the FLAG epitope ll ll as an N-terminal fusion with the protein of interest. The ... calmodulin resin. The vector has been improved by the addition of the FLAG epitope between the CBP purification tag and the protein of interest. This enables ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

... cleavage with EK to yield polypeptides with native amino acid sequence. Cleavage with thrombin yields the recombinant protein fused with the FLAG epitope for future analysis of the protein. The ESP LIC cloning kit contains pretreated, ready-to-use pESP-2 vector, Pfu DNA polymerase and 10X ...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

... a -FLAG immunodetection ll ll or by RNA detection using T3 antisense RNA probes; and the ability to seamlessly fuse the insert to the FLAG epitope and/or the HSP leader by using the seamless cloning kit** for which 80% cloning efficiencies are routinely achieved. Transient Expression ...