enhancer

Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

...ing Systems: Looking for Initial Clues Cis-acting enhancer elements usually bind more than onetranscriptional...ene product or extracellularstimulus with specific enhancer elements. Binding of an activated transcriptionfactor to specific enhancer elements, which are found...

Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )

Non-cytotoxic chemiluminescent enhancer for increased sensitivity Luminol-enhanced Assay for Superoxide Anion (O 2 ) Danny Q. Hoang Stratagene Lorraine C. Pfefferkorn Dartmouth School of Medicine, Dept. of Microbiology, Hanover, NH Stratagene introduces the LumiMax detectionassa...

Optimizing Transfection Conditions for Studying Signal Transduction Pathways

...stimuli or compoundsof interest on each particular enhancer element. Cell lines possess unique properties, su...endogenous transcription factors interact with the enhancer elements used in the PathDetect cis-reporting systems, a higher background often res...

Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements

...ng of an activatedtranscription factor to specific enhancer elements found in the promoters ofvarious cellular... signaling pathway converging at the corresponding enhancer elements. The five reporter plasmids ( figure 1 ) of the PathDetect cis-reporting sy...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

...mal PCR conditions. Stratagenes Perfect Match PCR enhancer * , improves the yield and specificity ofproblema...e. Weinvestigated the effects of Perfect Match PCR enhancer on amplificationsperformed with pfuturbo DNA polymerase,** , a robust high-fidelity PCR enzyme rec...

Mammalian and Bacterial Expression in One Vector

...yoticsystems. The vector contains the promoter and enhancer region of the humancytomegalovirus (CMV) immediate...ducible bacterial expression, the CMV promoter and enhancer region for constitutive mammalian expression and tandem consensus sequences for optimal translation ...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

...herprotein known as p48. This complex binds to the enhancer element, IFN-stimulatedresponse element (ISRE). In...etect cis-reportingsystems that use the cis-acting enhancer elements ISRE and GAS. PathDetect Cis-Reporting Systems All PathDetect cis-reporting systems fea...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

...lian cells. 3 The vectorcontains the promoter and enhancer region of the human cytomegalovirus (CMV) ### imme...nducible bacterial expression,the CMV promoter and enhancer region for constitutive mammalian expression, andtandem consensus sequences for optimal translation ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

..., growth factors, or drug candidates with specific enhancer elements. When a plasmid expressing the ge...ively ( Figure 2 ). Therefore, the enhancer element in the pNFAT-Luc plasmid is responsive to calcium mobilization. Cells transfected wi...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

..., growth factors, or drug candidates with specific enhancer elements. When a plasmid expressing the ge...ively ( Figure 2 ). Therefore, the enhancer element in the pNFAT-Luc plasmid is responsive to calcium mobilization. Cells transfected wi...

Genetic Reporter Systems

...l tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to transfect cells. Quanti...

Genetic Reporter Systems

...l tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to transfect cells. Quanti...

Enhancing PCR* Efficiency with MasterTaq

...lication describes the use of a new commercial PCR enhancer for improving PCR efficiency: the TaqMaste...nzymes, failed. The application of the PCR enhancer TaqMaster is highly recommended for solving problems with difficult targets, for detecting l...

The use of TaqMaster PCR Enhancer

...Eppendorf MasterTaq Kit contains the TaqMaster PCR enhancer that has been specially developed to impro... Figure 1. The TaqMaster enhancer makes the PCR signal both stronger and much more consistent. This is a 1.5 % agarose gel run...

The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA

...BSA), 1x reaction buffer, 1x TaqMaster PCR enhancer and 1U MasterTaq DNA Polymerase were adjusted to a final volume of 100 l with sterile water. From 80 ng to 500 ng of template DNA was added to the reaction mixture after preheating to 70C to avoid nonspecific annealing of ...

TransMessenger Transfection Reagent

...n conjunction with a specific RNA-condensing enhancer and an optimized buffer to form siRNA-TransMessenger Reagent complexes that are efficiently transferred into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and lo...

Effectene Transfection Reagent

... used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic ...

TransMessenger Transfection Reagent

...n conjunction with a specific RNA-condensing enhancer and an optimized buffer to form siRNA-TransMessenger Reagent complexes that are efficiently transferred into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and lo...

DNA Removal for Bioprocessing Applications

...ists identified a small molecule enhancer of TURBO DNase activity that further magnifies the...formance of TURBO DNase containing the new enhancer was compared to a competitors wild type DNase for removal of DNA from living cell cultures. ...

The World's Best DNase Improved TURBO DNA-free

... plague RT-PCR DNase enhancer increases the potency of TURBO DNase by 2-...possible DNA removal technology has resulted in an enhancer that increases the effectiveness of TURBO DNase by two orders of magnitude. This enh...