Digestion in Biological Technology

Pressure BioSciences, Inc. Reports Record Revenue, Significantly Reduced Operating Loss for the Fourth Quarter of 2008

... Q3 2009 release of our PCT-enhanced Protein digestion System, including the next generation small and ... development and sale of PCT-enhanced enzymatic digestion products designed specifically for the mass ... on the application of PCT-enhanced protein digestion for the mass spectrometry market and the ...

Environmental Power Corporation and Organix, Inc. Establish Alliance to Produce High Value Soil Amendment Products

... solids recovered from selected Microgy anaerobic digestion facilities. "This is a very exciting alliance," ... "Environmental Power has proven that their digestion model works and, like us, are interested in ... and other byproducts from their anaerobic digestion projects." ABOUT ENVIRONMENTAL POWER ...

Pressure BioSciences, Inc. Provides Corporate Update

... CEM, Inc. microwave-assisted technique for the digestion of a therapeutic monoclonal antibody. Amgen ... shown to be most effective at achieving complete digestion in a short time, without unintended perturbation ... Microwave digestion did not achieve complete digestion and induced oxidation of methionine residues." ...

Pressure BioSciences, Inc. Announces the Market Release of its PCT-Enhanced Trypsin Digestion Application for Proteomics

... cycling technology ("PCT")-enhanced trypsin digestion application for proteomics, a major new addition ... in the quality of the results of the digestion process. We have replicated these results in our ... commented: "The preparation of samples by trypsin digestion prior to mass spectrometry analysis is a new ...

Four Independent Research Groups Present Data Generated by Pressure Cycling Technology (PCT) at the ASMS Conference on Mass Spectrometry and Allied Topics in Denver, CO

... an on-line high pressure system -- on the tryptic digestion of proteins. They reported that complete digestion of proteins could be achieved in just 60 seconds. ... to current methods. Similar to the trypsin digestion study carried out by scientists at PNNL, ...

Max Muscle Introduces First-Ever Probiotics Protein Bar

... GanedenBC30 probiotic strain; research shows probiotics help with protein digestion and assimilation ANAHEIM, Calif., Feb. 10 /PRNewswire/ -- National ... "We have long known the health benefits probiotics deliver in improving digestion and enhancing immunity, but we are just now learning about the ability to ...

Environmental Power Holds Ground Breaking Ceremony for Renewable Natural Gas Facility at Rio Leche Estates

... of oil. The facility will use a thermophilic, codigestion anaerobic digestion process to generate methane-rich biogas, condition the gas to natural gas ... America for the development and deployment of a proprietary anaerobic digestion technology for the extraction of methane gas from animal wastes and other ...

DOE JGI plumbs termite guts to yield novel enzymes for better biofuel production

... hindguts to break down the cell walls of plant material and catalyze the digestion process. Industrial-scale DNA sequencing by DOE JGI was key to ... these compartmentalized, complex microbial communities required for wood digestion is now coming to light. Like cows, termites have a series of stomachs, ...

Ganeden Biotech, Liquid Health Labs Unveil New Drink Technology

... Ganeden BC30 (R), as well as ingredients that have been previously unavailable to the beverage industry due to manufacturing constraints. "With digestion and immunity continuing to be important health topics for many consumers, and the challenges of using probiotics in traditional beverages, developing ...

Pressure BioSciences, Inc. to Present at the EdgeWater Research Partners' Spring 2009 Micro/Small-Cap Investor Conference

... reactions, immunodiagnostics, and protein purification. PBI currently focuses its efforts in the development and sale of PCT-enhanced enzymatic digestion products designed specifically for the mass spectrometry marketplace, as well as sample preparation products for biomarker discovery, soil and plant ...

Pressure BioSciences, Inc. Announces Completion of Private Placement

... Company's ability to achieve its business goals, the effect of the Company's decision to focus primarily on the application of PCT-enhanced protein digestion for the mass spectrometry market and the advantages of PCT in this market, and the use of PCT in biomarker discovery, soil and plant biology, ...

EAU Technologies Receives Patent for Empowered Water(TM)

... that are being used on dairies to process drinking water for dairy herds. Studies and trials are showing promising results at improving animal digestion which shows signs of improved animal health and production. EAU uses terms like "green", "natural", "non-toxic" and "organic" based on our NAMSA ...

Pressure BioSciences, Inc. and the J. Craig Venter Institute Announce Collaboration Agreement & Purchase of a PCT System

... Inc., commented: "We are very pleased that this large, global biopharmaceutical company has purchased two PCT Systems for accelerated and accurate digestion of therapeutic proteins, one of PBI's major product initiatives. We believe that this application may lead to better quality control in the ...

Oberon FMR Wins Top Prize at BioWest 2008 Venture Showcase Competition

... but can also extend towards less easily quantified, though meaningful benefits. These benefits include: 1) elimination of hard costs associated with digestion and transportation of solids generated during wastewater treatment, 2) avoidance of soft costs such as possible exposure to negative public relations ...

UCF professor develops vaccine to protect against black plague bioterror attack

... protein found on the outside of Yersinia pestis. The vaccine was inside the plant cells, which were given to the rats. The vaccine was protected from digestion in the stomach and was then absorbed in the gut. It kick started the immune system into producing antibodies, which protects against the deadly ...

UCF professor develops vaccine to protect against black plague bioterror attack

... protein found on the outside of Yersinia pestis. The vaccine was inside the plant cells, which were given to the rats. The vaccine was protected from digestion in the stomach and was then absorbed in the gut. It kick started the immune system into producing antibodies, which protects against the deadly ...

Pressure Cycling Technology (PCT) Highlighted in Five Podium Presentations at the Fifth International Conference on High Pressure; PBI Introduces the Patent-pending 'PCT Shredder' at the Meeting

... the significant advantages offered by PCT in the study of proteins in his presentation entitled "Application of Pressure for Ultra Fast Protein digestion in Proteomics." Finally, Dr. Yiping Cao of the Southern California Water Research Project (SCWRP) gave a presentation entitled "Comparison of ...

Breakthrough Slashes $7 Corn Over Half for Cattle Feeders

... process in their rumen, because it is already wet and pre-digested - this makes nutrients more readily available and improves feed value too. Also, digestion is aided by the naturally occurring L-form Lactic Acid that turns to propionic acid in the cow's rumen. Because Sila-Max earlage tastes sweet ...

Main Street Gourmet Launches New Probiotic Muffins

... has found GanedenBC30 to be Generally Recognized As Safe (GRAS) in use as a food ingredient. In clinical studies, GanedenBC30 has been shown to aid digestion and improve immune function when used regularly. Companies such as Main Street Gourmet continue to provide evidence that GanedenBC30 can be ...

Bion Outlines Differences between its Patented Comprehensive Livestock Environmental Treatment Technology and Anaerobic Digesters

... and performance of its comprehensive livestock environmental treatment technology compared to anaerobic digesters. Anaerobic or methane digestion (AD) has been used to produce energy from livestock waste since the late 1800s. While its efficiency has improved over the years, it is still based ...

Environmental Power Announces Opening of New Corporate Headquarters in Tarrytown, NY

... operating subsidiary, Microgy, Inc., holds an exclusive license in North America for the development and deployment of a proprietary anaerobic digestion technology for the extraction of methane gas from animal wastes and other organic waste for its use to generate energy. For more information visit ...

China-Biotics, Inc. Launches Shining Probiotics Protein Powder

... Probiotics Protein Powder. Each has more than 30 micronutrients and contains four types of Shining Probiotics, all of which are designed to improve digestion and the body's ability to absorb protein. "We are delighted to offer customers this newly developed nutritional product," said Mr. Jinan Song, ...

Dairy Farm Buys Technology to Turn Manure into Renewable Energy

... a digester system based on Microgys anaerobic digestion technology, which processes the manure produced by their dairy operations. Anaerobic digestion is a method of producing renewable energy ... went into effect in August. Anaerobic digestion systems help animal feeding operations meet ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... the PCR product following restriction digestion (data not shown). The recognition ... were confirmed by restriction enzyme digestion of the PCR product at a BstN1 site that spans the ... to that determined by restriction digestion (data not shown). ...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

... domains. 26 However, the initial digestion product contains a wide mixture of proteins, ... and accumulate low molecular weight digestion products of the human platelet TXA 2 ... over methionine residues in the protein, with digestion carried out at room temperature, while ...

Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

... sample. Efficient protease digestion conditions allow RNA isolation in less than ... FFPE Tissues ), the protease digestion conditions are designed to ... as easily as RNA by simply increasing the digestion time to 2 days. Isolation of DNA ...

DNA-free A NEW Method to Remove DNA

... DNase, and an optimized DNase digestion buffer, to ensure safe, complete removal of ... or inactivation of DNase after digestion include: heat inactivation, proteinase K ... the divalent cations in the DNase digestion buffer can cause ...

Determinants of RNA Integrity and Purity

... determine the rRNA ratio. Incomplete DNase I digestion can generate small molecular weight ... C). The most common causes of incomplete DNA digestion are residual contaminants (high salt, ... generated by incomplete DNase I digestion (C) . ...

Five Ways to Produce siRNAs

... 2. In vitro transcription 3. digestion of long dsRNA by an RNase ... RNase III digestion of dsRNA ... siRNA Design All of the above methods, except digestion of long dsRNA by RNase III, ...

Dicer vs. RNase III for Preparation of siRNA Cocktails

... is a rather inefficient enzyme, requiring long digestion times (12-16 hour). In contrast, RNase ... Figure 1. 12-15 bp RNase III digestion Products Elicit Silencing. A 200 bp ... RNase III (30 U) for 1 hour at RT. digestion products were run on a 15% non-denaturing ...

Inducing RNAi with siRNA Cocktails Generated by RNase III

... product length generated by RNase III digestion can be increased by altering digestion conditions, and it has ... cocktails produced by complete digestion by RNase III (~12 to 15 bp) ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... proteins or phenols. No inhibition or partial of digestion can be seen with the cosmids isolated ... Lane 3 Hind III Marker; Lane 4 7, digestion of H115H12, H130A02, H104E08, H107G01 ... 8, 1 Kb ladder; Lane 9 HindIII Marker; Lane 10, digestion of H9305 with BamHI ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... If desired, the FLAG tag can be removed by digestion with enterokinase, leaving protein of native ... does not require ligation or restriction enzyme digestion ( Figure 2 , Panel A). The pCAL-n-FLAG ... 3 , Panel B). Removal of CBP Tag by Thrombin digestion and Anti-FLAG Western Blot of FLAG-JNK The ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... When enzymes are tested under standard digestion conditions (1 U of enzyme/g substrate DNA), ... removed using StrataClean resin. The digestion products are ligated using Stratagenes T4 DNA ... of the DNA ends produced during the digestion reaction is compromised (i.e., a ...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

... created three unique restriction enzyme digestion sites. Plasmid DNA isolated from 9 out of 12 ... at 37C for 1 hour. Following Dpn I digestion of parental DNA, 1- and 4-l volumes of ... Bgl II, Pst I, and Xho I restriction digestion patterns for seven of the clones are shown in ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

... to the instructions in the PicoPure kit, including the on-column DNase digestion detailed in the user guide Appendix A. Each RNA sample was eluted in 11 L ... 3. Antisense probes were generated by restriction enzyme digestion and transcription from the T7 promoter in the presence of P 33 UTP. ...

Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

... detection, fractions were completely mineralized by an open, wet digestion step with nitric acid, perchloric acid, and sulfuric acid. ... in separated and stained protein bands after PAGE, followed by digestion of the excised gel segments. 5 No other fractionation technique ...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

... The aspirated cells/buffer were transferred to 5 l proteinase K digestion buffer (20 g proteinase K/ml in 10 mM Tris/HCl, 0.5% Nonidet P40 ) in PCR reaction vials. digestion was performed for 10 minutes at 55 C to demask the DNA followed ...

Genomic DNA Preparation from RNAlater Preserved Tissues

... Protocol Prepare digestion buffer: 60 mM Tris, pH 8.0 100 mM EDTA 0.5% SDS ... digestion. Place in a 2.0 ml eppendorf tube with 1.5 ml digestion buffer. Add Proteinase K to a final concentration of ...