detached

Yeast Protein Production System Features High Yields and One-Step Purification

...ite results in fusion proteins that can be detached from the GST tag with either protease. Cleavage by...usion protein. The GST tag was efficiently detached from the MEK1 moiety by digesting the purified GST-MEK1 fusion protein with enterokinase. Th...

DNA Removal for Bioprocessing Applications

...,000/well) were incubated with 5 U of TURBO DNase, detached from the plate, and Viacount cell dye was added to differentiate live and dead cells. Cells (1000) were counted by microcytometry in a Guava Technologies PCA 96 instrument. Conclusions: Ambions exclusive...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

.../He system is efficient for inoculation of detached leaves, but the Helios gene gun enables inoculation of an intact plant. In this study we have used the Helios gene gun to inoculate whole tobacco and potato plants with the cDNA of an RNA virus, potato virus A (PVA; Puurand et...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

...ession of Mlo in single epidermal cells of detached barley leaves. Barley Mlo is known to damp... target shelf carrying the petri dish with detached leaf segments was placed 6 cm from the Hepta adaptor. Usually 810 leaf sections (barley) or ...

Aurum Plasmid 96 Kit Tech Note, Rev A

...te, the lysate filtration plate was simply detached and discarded, without having to disassemble the manifold. Moreover, no extra time was spent binding the plasmid to the plasmid binding plate; lysate filtration and DNA binding were accomplished simultaneously. Since lysate fi...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

...ay, suitable target cells in log-phase growth were detached by treatment with trypsin. Cell concentrations were adjusted to suitable levels with culture medium. The adenoviral vector product was thawed by placing the tube on ice, added to the cell suspension, and mixed to provide the appropriate multiplicity ...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

...ay, suitable target cells in log-phase growth were detached by treatment with trypsin. Cell concentrations were adjusted to suitable levels with culture medium. EGFP-MAPKAP-k2 adenoviral vector was thawed by placing the tube on ice, added to the cell suspension, and mixed to provide the appropriate multiplici...

Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

...ay, suitable target cells in log-phase growth were detached by treatment with trypsin. Cell concentrations were adjusted to suitable levels with culture medium. Ad-A-Gene CRE-NTR vector was thawed by placing the tube on ice, added to the cell suspension, and mixed to provide the appropriate multiplicity of in...

Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening

...plate. Cells expressing apoaequorin and a GPCR are detached from the culture plate (if they are adherent) and are incubated with coelenterazine to reconstitute active aequorin. These are then maintained in suspension with a stirrer and the cell suspension is injected, well by well, on the solutions of tested ...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

... 20 minutes with 3 mls of 0.526mM EDTA in PBS. The detached cells were harvested and diluted in PBS. An aliquot was counted using a hemocytometer. The concentration of cells was adjusted to 2.5 x 10 5 cells/ml in PBS, which is equivalent to a starting concentration of 2.5 x 10 4 cells/100l. For prep...

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

...ion or contamination. Now that the vessel is detached from the unit, it can be cleaned. To do this it wi...emoved at this point as well. The sampling tube is detached and washed separately.The valve on the sampling port and all clamps on all tubing connected to ports...