Crassa in Biological Technology

Efficient Transfection of Neurospora Crassa

... nutritional conventions of Neurospora crassa have made this filamentous fungus a model ... than a half a century. In fact, N. crassa was used in the Nobel Prize-winning "One-gene One-enzyme" theory [1] . Since then, N. crassa has been used as a model organism in ...

Efficient Transfection of Neurospora Crassa

... nutritional conventions of Neurospora crassa have made this filamentous fungus a model ... than a half a century. In fact, N. crassa was used in the Nobel Prize-winning "One-gene One-enzyme" theory [1] . Since then, N. crassa has been used as a model organism in ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... [3] was used in this experiment for screening of the Neurospora crassa genome. Restriction analysis of the pLorist6Xh vector isolated ... New York, 2000. Kelkar, H.S., et.al., The Neurospora crassa Genome: Cosmid Libraries Sorted by Chromosome, Genetics 157, ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... being used for protoplast formation or for microprojectile bombardment. Plasmid pRG-1, a 5 kb plasmid containing the Neurospora crassa pyr4 gene cloned into pUC9 was used for transformation experiments. This plasmid has been shown to complement the pyrG89 ...