Cosmid in Biological Technology

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... in conjunction with the Human Genome Project. cosmid vectors are constrained to 4045 kb by ... Six plasmids were used: pUC19 (2.6 kb), 7 cosmid tsA/Y/1d4 (44 kb), 8 and F factor ... deletions in recombinant cosmid DNA propagated in Escherichia coli, J. ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... a rapid and effective way of purifying cosmid DNA from bacterial culture. ... ranging from 30kb and 45kb DNA [1]. cosmid structures were created from a hybrid of ... to find a method that will purify the cosmid without shearing or excessive loss of DNA. ...

Easy Cosmid DNA Purification with StrataPrep Kits

... easily purify very large plasmids, including cosmid DNA. To demonstrate cosmid purification, a SuperCos I clone containing a ... using the StrataPrep EF plasmid midiprep kit. The cosmid DNA yield from the two cultures was 45 ...

Plasmid Miniprep Kit Selection

... For high- and low-copy plasmid and cosmid DNA preparations of * Option to automate For use on robotic workstations ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

QIAfilter Plasmid Kits

... For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA Features and benefits Up to 10 mg of ultrapure plasmid DNA Cleared lysates from up to ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

EndoFree Plasmid Kits

... For purification of endotoxin-free ultrapure plasmid or cosmid DNA Features and benefits Less than 0.1 EU/g plasmid DNA Yields of up to 10 mg plasmid DNA ...

HiSpeed Plasmid Kits

... For ultrafast purification of up to 750 g plasmid or cosmid DNA Features and benefits Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

... 1.5- to 3-ml liquid cultures, purified plasmid DNA can be obtained in an average of 2.5 minutes per miniprep. Plasmid and cosmid DNAs isolated with the StrataPrep plasmid miniprep kit are easily digested with restriction enzymes ( figure 2 ). ...