complementation

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...-inhibition Phenotypic complementation Signaling proteins ...-inhibition Phenotypic complementation Reporter activation Transcription factors ...

Generate High-Quality, Directional Plasmid cDNA Libraries

...tions, such as yeast two-hybrid screening, complementation studies and expressed sequence tag (EST) sequencing, cDNA libraries constructed directly into plasmid vectors are convenient and easy to use. However, constructing high-quality plasmid libraries can be limited by the efficienc...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

...04 (1999) Shirasu K et al., Cell-autonomous complementation of mlo resistance using a biolistic transient expression system, Plant J 17, 293299 (1999) Zhou F et al., Molecular isolation and functional analysis of the barley Mlo1 powdery mildew resistance gene by using a transien...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

...words: cAMP • GPCR • enzyme fragment complementation • LEADseeker • assay miniaturization...ay for screening at GPCRs based on enzyme fragment complementation (EFC) technology and cyclic adenosine 3’, 5’ monophosphate (cAMP) measurement. The data ...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

...ine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent ...mpetitive binding assays utilizing enzyme fragment complementation (EFC) for the sensitive and simple measurement of a range of biologically important targets. Based o...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... of assay kits, which are based on enzyme fragment complementation technology, perform well in conjunction with the chemiluminescent readout of LEADseeker Multimodality Imaging System equipped with the supplied luminescence filter. Used together, the components support organizations interested in adding high and ult...