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coefficient in Biological Technology

Zeus Announces Product Name Changes

... is a micro-porous material that is medically implantable and has a low coefficient of friction. The new brand name Aeos was coined after the word ... of PEEK tubing and can be used anywhere PEEK is used with a need for lower coefficient of friction. The material's new name suggests the product's excellent ...

Microtest Labs Expands Clean Room, Contamination Testing Services

... to ISO 14644 guidelines, the EU Annex, or both. Disinfection validation , including dilution testing, phenol coefficient testing, sporicidal testing and germicidal testing. Gowning validation as well as assistance with developing ...

Frost & Sullivan Recognizes Novagali Pharma for Innovation in Ophthalmic Therapies

... anterior segment as well. Compared to conventional eye drops and anionic emulsions, Novasorb formulations exhibit a four-fold higher spreading coefficient allowing for increased wettability of the eye's surface while also protecting against mechanical damage that occurs from blinking. This increase in ...

JD Technologies, LLC Selected by FCT Ingenieurkeramik GmbH to Represent Their High Performance Ceramic Expertise In the United States

... performance due to high temperature & corrosion resistance, no or low wear, high stiffness and strength, low density and weight, a very low coefficient of thermal expansion, excellent thermal shock behavior and improved biocompatibility. Significant life cycle cost savings are attained due to the ...

University of Pennsylvania engineers reveal what makes diamonds slippery at the nanoscale

... the sliding experiment. The team tested a thin film form of diamond known as ultrananocrystalline diamond and found super low friction (a friction coefficient ~0.01, which is more slippery than typical ice) and low wear, even in extremely dry conditions, (relative humidity ~1.0%). Using a microtribometer, ...

Singulex Collaborates With Large Pharmaceutical Company in Erenna(TM) Technology Access Program

... Erenna system to improve and validate their vital immunoassays, adding as much as 1-3 logs of improvement in sensitivity and providing less than 20% coefficient of variability. ETAP includes the Erenna system, which is designed to detect and count single molecules, customized immunoassay development ...

Tiny avalanche photodiode detects single UV photons

... detection from a III-nitride photodetector. These back-illuminated devices, based on GaN compound semiconductors, benefit from the larger ionization coefficient for holes in this material. The back-illumination geometry will facilitate future integration of these devices with read-out circuitry to realize ...

Advanced Instruments Introduces World's Fastest High Throughput Freezing Point Osmometer

... cabinet -- Random-access, fully programmable system controls -- Statistical analysis components that provide mean, standard, deviation and coefficient of variation functions -- Data export -- LIMS user interface -- Flexible reporting options and results storage/retrieval -- Barcode reader that ...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

... by the in-house standards showed a correlation coefficient (r) of 0.991 (Figure 1). The correlation coefficient was not improved by extension of the ... by the in-house standards showed a correlation coefficient (r) of 0.996. This could be improved ...

The iCycler iQ Detection System for TaqMan Assays, Rev B

... standard deviation of 0.6 cycles and a coefficient of variation (CV) of 2.6%. However, these ... the standard curve has a correlation coefficient (r 2 ) of 0.995, but the 2 most ... standard curve shows a correlation coefficient of 0.998 over a range of 8 orders of magnitude ...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

... -2.449 or 156% efficiency) and a poor correlation coefficient (r = 0.950). An amplification ... efficiency). However, the correlation coefficient remained poor (r = 0.951). Collecting data at 82C ... demonstrated an excellent correlation coefficient (r = 1.00) with a very high efficiency ...

FERMENTATION BASICS

... by the oxygen tranfer rate (OTR) or by the volumetric oxygen transfer coefficient (k L a) . These values have been thoroughly examined as a critical ... function. The oxygen transfer rate and the volumetric oxygen transfer coefficient are dependent on the following parameters: * the ...

Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80

... column that had seen over 500 injections. As expected, the coefficient of variation for the retention time of the various peptides between injections averaged below 0.4% for both columns. The coefficient of variation for peak heights, which relates directly to ...

Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

... the following equation: y = 5.5997 10 4 + 0.0206125x. The correlation coefficient for this curve was r 2 =0.9999, indicating excellent linearity across the ... as a percent relative error, is 1.1% and the precision, as a percent coefficient of variance (%CV), is 12.1% for five replicate injections. Above the LOQ, ...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

... ribosomal RNA assay. Precision and Reproducibility of Recovery coefficient of variation (%CV): Total experimental coefficient of variation, including 6700 workstation purification, all pipetting steps ...

Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

... concentration range (n=12). The calculated recovery was about 70% with a coefficient of variation of 5%. The precision was determined using ... The number of replicates of each concentration was 10. Table 2 shows the coefficient of correlation for all 4 MRM transitions. The coefficients of correlation ...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

... were linear within their designated ranges with a correlation coefficient of ≥0.99. Bio-Rad's detergent compatible (DC) ... were linear within their designated ranges with a correlation coefficient of ≥0.99. Conclusion The ...

Benchmark Plus Microplate Reader Scan Well Feature

... nm on Benchmark Plus yields a linear graph with a correlation coefficient of 0.9993 (Figure 2). ... the optimal wavelength for the bovine IgG tested, the correlation coefficient is ≥ 0.99 and linear over the dilution range of 62.5 g/ml ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

... MW, and sizing reproducibility, which was evaluated using the coefficient of variation, or CV ([standard deviation/mean] x 100), as a ... software package, a linear fit of the data was created and a coefficient of determination, r 2 , was calculated. On average, the ...

Real-Time PCR: General Considerations, Rev A

... a slope of -4.53 or 66% efficiency, with a correlation coefficient of 0.995 (Figure 2, upper panel). Moving the reverse primer ... of the reaction to 99% (slope = -3.35) and a correlation coefficient of 0.999 (Figure 2, lower panel). ...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

... 310 Genetic Analyzer or agarose gel electrophoresis methods. The mean coefficient of variance (CV) for these three methods was 0.26, 0.39 and 0.50, ... methods is that the CV for each method was so low. If the coefficient of variance (CV) for each method (agarose gel, ABI Prism 310, Agilent ...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

... against concentration, weighted 1/X (Figure 6). A correlation coefficient of r2=0.9990 with an equation of Y = 8496.82+266143*X was ... against concentration, weighted 1/X (Figure 10). A correlation coefficient of r2=0.9994 with an equation of Y = 2661.76+85951.1*X was ...

Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results

... temperatures at a rate of approximately 0.67C/second, with a coefficient of variation (the standard deviation divided by the mean) of ... RoboCycler system was approximately 0.85C/second with a 0.22% coefficient of variation between tubes. This translates to a 27% increase ...

Near Infrared Technology and Optical Agents For Molecular Imaging

... compounds cause tissue a u t o f l u o rescence throughout the visible spectral range up to approximately 700 nm 5, 6 . Because the absorption coefficient of tissue is considerably smaller in the near infrared region (700 nm-900 nm), light can penetrate more deeply into the tissues to depths of ...

Serial Dilution with the Bravo Liquid Handling Platform

... fluorescein reservoir is placed on position 7. Fluorescence measurements from each well are used to determine the precision of the transfer. coefficient of Variance (CV) calculations are made by dividing the standard deviation by the mean for each column/row. Accuracy is calculated based on an ...

XANTHOCHROMIA IN CEREBROSPINAL FLUID

... compensates for the interference of haemoglobin and bilirubin absorption bands. Figure 1: Molar Extinction coefficient of Haemoglobin (left) and Bilirubin (right) [cm-1/M vs nm] The method consists in scanning the region 350-550nm and printing the ...

The New Affymetrix GeneChip Scanner 3000

... sites and on both scanner types. Thus, all 22,283 probe sets from the HG-U133A probe array and their Signal values were used for each correlation coefficient determination. Correlation coefficients calculated for each scanner comparison, chip design, and site are summarized in Table 2. The high ...

Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

... over the entire range of the assay is shown in Figure 5, with the linearity over the dynamic range from 500 fg to 5 ng on-column. The correlation coefficient is 0.9991. The precision values for the alprazolam assay are displayed in Table 1. The % CV or RSDs show that the LCQ Deca XP Plus ion trap mass ...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

... tended to be more variable with Fluo-3 (see Figure 1). With Fura-2 there was a 4.8 fold increase in signal above basal. The screening coefficient window (Z-factor), which reflects the dynamic range of the signal and the data variation for the assay 9 was calculated using the buffer addition ...

Gq-coupled Receptor Assays Using FLEXstation

... day and by less than fourfold for experiments performed on different days. The range of values from all experiments was between 24 and 87 mM. The coefficient of variation of replicate samples within an experiment varied from 5.6 to 13.8% (average 8.6%) for the 1 mM ADP concentration. ...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

... volume of diluent and the absorbance measured. The actual dispensed volume is then calculated from the absorbance, the light path, the extinction coefficient and the diluent volume. Various chromogens have been used, including Ponceau S dye, methyl red, Evans Blue dye and nicotinamide. 1 ,2, 10, 11 ...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

... Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm ....

Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method

... if desired, to determine percent usage of the drug (data not shown). Excellent linearity is seen within this range with the average correlation coefficient being 0.993. Accuracy in retention times of the metabolites is also excellent with an average 1.15% RSD, the dextrorphan being slightly more variable ...

Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data

... (and/or technical replicates), which rejects array outliers based on regression threshold. For example, the data point that has a correlation coefficient of R2 Step 2: Perform t-test/ANOVA analysis to determine differentially expressed genes based on p-value threshold (e.g. p Step ...

Detection of nitrofurane metabolites in food with HPLC/MS/MS

... all metabolites a calibration curve from 0.5 pg to 750 pg. We determined linear regression coefficients (weighed by 1/x) of 0.999 and better. Similar coefficient were found on the API 2000 system in a concentration range of 50 pg to 5 ng. Summary The observed ...

Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

... c = concentration O.D. = absorption value determined ε = extinction coefficient of the relevant fluorescent dye

Following determination of concentration, each dye was diluted further in buffer to a top working ...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

... >Data from each test compound well were normalized to agonistonly controls on the same assay plate. Plate quality was assessed using the Z` coefficient (6) calculated from controls on each plate. Seven of the eight plates had a Z` = 0.5, as shown in Figure 2. No false positives were detected on ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... desired, we recommend ECL Plex goat-α-rabbit IgG-Cy5.

To determine the linearity of the data the square of the correlation coefficient (R 2 ) was used. Pixel intensities from the scanned membranes were plotted against the amount of protein loaded onto the gel, and a linear curve fit ...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

... but still in the exponential growth phase of the PCR. The C T vs. log (concentration) plot was linear with a large correlation coefficient (r = 0.986), showing that the assay correctly and accurately reflects the amount of PSA in the studied range. However, PCR ...
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