Clones in Biological Technology

OriGene Announces 37,000 ORF cDNA Clones in a GFP Vector

... over 25,000 human ORF (open reading frame) clones and 12,000 mouse ORF clones can be ordered in pCMV-tGFP vector. In early ... to providing expanded utilities for these clones in life science research and drug discovery. ...

Cyntellect Launches CellXpress(TM) System to Boost Productivity of Biopharmaceutical Process Development

... of cells per day and often yield tens of clones following many weeks of growth and assessment. ... Yielding verified highly-secreting single-cell clones in less than 24 hours (vs. weeks) -- Isolating ten-fold more highly secreting clones than traditional methods -- Selecting ...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

... vector constructs. Currently, more than 5,000 clones have been tested in the functional validation process. Validated clones covering more than 700 genes are available in ... collection, a number expected to grow by 75,000 clones per year through 2011. TRC2 will also generate ...

Animal Protection Groups Condemn FDA's Endorsement of Animal Cloning

... swells with fluid. Over 50 percent of all calf clones suffer from Large Offspring Syndrome, in which ... umbilical, and immunologic problems." Even older clones who appear healthy have been known to develop ... However, as shown in a 2007 study, 42 percent of clones died despite receiving extensive veterinary care. ...

Top U.S. Cloning Companies Announce New System to Track Cloned Livestock

... products of cloned animals. "Because so few clones currently exist, putting this system into ... or sale to a packer/processor that accepts clones (verified by signed statement from ... risk assessment stating that food derived from clones and their offspring is indistinguishable from ...

GeneCopoeia Introduces Genome-Wide MicroRNA (miRNA) Functional Analysis Tools

... gene. miTarget(TM) miRNA target sequence (3' UTR) expression clones cover genome-wide human, mouse and rat genes. These expression ready ... human and mouse full length protein coding ORF cDNA clones , which are offered to customers in more than 80 vectors with a choice of ...

Animal Eggs Not Suitable Substitutes to Produce Stem Cells

... not produce the same results. Although the human-bovine and human-rabbit clones looked similar to the human-human embryos, the human-animal hybrids did not exhibit the changes in gene expression seen in the human-human clones and normal embryos. Specifically, they did not achieve up-regulation of ...

Tips on how to build a better home for biological parts

... database but also the collection of publicly available DNA sequences or clones (BioBricks) that are used to make the biological devices and systems. The analysis of the Registry database and the associated DNA clones identified several key needs where improvements could be made. These ...

Tips on how to build a better home for biological parts

... database but also the collection of publicly available DNA sequences or clones (BioBricks) that are used to make the biological devices and systems. The analysis of the Registry database and the associated DNA clones identified several key needs where improvements could be made. These ...

Statement on the Release of the European Food Safety Assessment on the Safety of Food Derived From Clones and Their Offspring in the Food Supply

... Food Safety Authority's strong endorsement of the safety of food from clones and their offspring, we urge the U.S. Food and Drug Administration to ... and New Zealand have published risk assessments, finding the foods from clones and their offspring to be safe and equivalent. -- Australia has ...

PNH Patients Treated with Soliris(TM) Experienced Dramatic Reduction in Blood Clots During Clinical Trials

... in patients with PNH, and patients with both smaller and larger PNH clones are reported to experience this complication.(8,9,10,11,12) Research ... (16) Iwanga, et al. Paroxysmal nocturnal haemoglobinuria clones in patients with myelodysplastic syndromes. Brit J Haem. 1998; ...

SAFC Biosciences(R) Expands Media Capability for Chinese Hamster Ovary (CHO) Cell Lines

... 20 cGMP media formulations for CHO cells that can be quickly accessed for media screening optimization projects. Historical data shows that CHO clones have diverse nutritive requirements and that no single media formulation is optimized for every application. According to Archie Cullen, Vice ...

Sigma-Aldrich Introduces MISSION(R) LentiPlex(TM) Pooled shRNA Libraries to Enable Rapid Whole Genome Screening in Primary Cells, Stem Cells or Tumor Cells

... human (MISSION TRC-Hs1.0) and murine (MISSION TRC-Mm1.0) collections of whole genome arrayed libraries, gene family sets or individually prepared clones in glycerol, purified DNA or lentiviral formats. For more information on the MISSION LentiPlex Pooled shRNA Library, please visit ...

Soliris(R) Reduced Measures of Thrombosis and Inflammation, and Decreased Indicators of Pulmonary Hypertension, in Studies of Patients with PNH

... failure syndrome. Blood. 2002;100 (12):3897-3902. (8) Iwanga M, Furukawa K, Amenomori T, et al. Paroxysmal nocturnal haemoglobinuria clones in patients with myelodysplastic syndromes. Br J Haematol. 1998;102 (2):465-474. (9) Maciejewski JP, Risitano AM, Sloand EM, et al. ...

Alexion's Soliris(R) Receives 2008 Prix Galien USA Award for Best Biotechnology Product

... hemoglobinuria-type cells in bone marrow failure syndrome. Blood 2002;100:3897-3902. (7) Iwanga, et al. Paroxysmal nocturnal haemoglobinuria clones in patients with myelodysplastic syndromes. Brit J Haem. 1998; 102:465-474. (8) Maciejewski, et al. Relationship between bone marrow ...

Biotest Diagnostics Receives FDA Clearance for Full Line of Traditional Blood Bank Reagents

... anti-Lea, anti-K, anti-k, anti-Fya, anti-Jka, anti-Jkb, anti-M, anti-N, anti-S, anti-s and anti-P1. All antisera are monoclonal and many of the clones have never been previously available in the U.S. Biotest's reagents are recognized for their high levels of sensitivity and specificity and are ...

Venter Institute Scientists Create First Synthetic Bacterial Genome

... to produce large fragments approximately 144,000 base pairs or 1/4th of the whole genome. At this stage the team could not obtain half genome clones in E. coli, so the team experimented with yeast and found that it tolerated the large foreign DNA molecules well, and that they were able to assemble ...

Stemagen First to Create Cloned Human Embryos From Adult Cells

... said French, who is Stemagen's Chief Scientific Officer. Five blastocysts were developed from 25 donated mature oocytes. Three were confirmed to be clones based on DNA fingerprinting demonstrating the presence of the skin cell donor DNA in the blastocyst, while one was further confirmed to be a clone by ...

Start Licensing, Inc. Welcomes FDA's Finding of Safety of Food From Cloned Animals

... risk assessment released by the US Food and Drug Administration on January 15. The assessment concluded that meat and dairy products from animal clones and their offspring are safe for human consumption. "FDA's conclusion is consistent with a report released January 11, 2008 by the European Food ...

Nanofabrication method paves way for new optical devices

... open the possibility of ultra-small sources of light. Furthermore, given their precise organization, they can serve as templates for making their own clones or for making other ordered structures at the nanoscale, such as arrays of nanoparticles. This work is exactly the kind of high-risk, ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

... 1. Based on the sequence information for the clones identified as differentially expressed on the microarrays, PCR was used to amplify select clones using oligodT primed cDNA as template. PCR was ... Based on the sequence information for the clones identified as differentially expressed on the ...

Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies

... or phage-derived artificial chromosome (PAC) clones (verified by fluorescence in situ ... to a glass slide. Three or more different clones were used per region of interest. Genomic DNA was ... as detected by 14 out of 15 determined target clones in the array for both unamplified and amplified ...

cDNA Sequencing & Finishing

... for rapid 5 cDNA screening. Key cDNA clones identified during screening are easily integrated ... AND FINISHING Purified cDNA clones are sequenced using BigDye Terminator v3.1 ... at identifying and sequencing cDNA clones containing a full-length open reading ...

Drive Long-Term Silencing with CMV Vectors

... difficulty in producing such clones is possible interference between the pol II and ... promoters can occur. This suggests that clones derived from a ... p Silencer 4.1-CMV transfected clones had reduced GAPDH protein and mRNA expression ...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... by sequencing of all restriction-positive clones using the primer 5'-TGAATTTTCTGTATGAGG-3', the ... Although, in theory, 100% correct clones can be obtained by a strategy involving ... orientation, a notable number of false-positive clones arose in all our ligation experiments. Most of ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

... plaque hybridization techniques, cDNA clones with an incomplete 5' end are ... in the scheme in Fig. 1. As all cDNA clones in the library act as a template for the vector ... (TA Cloning Kit, Invitrogen). Ten clones selected at random with inserts were sequenced ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

... plaque hybridization techniques, cDNA clones with an incomplete 5' end are ... in the scheme in Fig. 1. As all cDNA clones in the library act as a template for the vector ... (TA Cloning Kit, Invitrogen). Ten clones selected at random with inserts were sequenced ...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

... clones. Amplification directly from clones in culture has been recently reported (3). This ... PCR amplification Selected clones were inoculated into 96-well Falcon U-bottom ... GEM 1 Clone List (build 35) containing 8,734 clones was purchased from Incyte ...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

... clone collection. This collection consists of clones from our innovative libraries and contains a high number of clones that do not have significant nucleotide homology ... public databases are also assigned an EST number. clones not having significant nucleotide homology to ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... function more efficiently with standardized clones Rebecca L. Mullinax Heidi Davis David ... cDNA in a single dual- expression vector. These clones eliminate the time spent cloning, sequencing, ... Availability GeneConnection expression-tested clones were selected based on their biological ...

hNT BlueNeurons Express b-Galactosidase at High Levels

... NT2 precursor cells, select stably transfected clones and differentiate the clones into hNT neurons. figure ... promoter. Generating Stably Transfected NT2 clones Figure 3 NT2 ...

Generate High-Quality, Directional Plasmid cDNA Libraries

... cDNA. Transformation of plasmid clones is significantly improved by the use ... 2). The average number of primary clones obtained from transformation into ... greater than the number of primary clones obtained from XL2-Blue cells and 22-fold greater ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... levels and the distribution of insert size. The clones examined contained an average insert ... previously described. In each case, over 1000 clones were analyzed, then the clones were characterized into several classes ( Table ...

The GeneZoo or the GeneJungle?

... more than 50% are estimated to be full-length clones Jason Goncalves Jean-Michel Lelias Joe ... have systematically sequenced the ends of our clones and have used a rigorous cDNA clustering algorithm to determine how many of our clones are unique and how many can be found in ...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

... target cell should yield a high frequency of clones capable of mediating desirable expression ... time and labor required to screen for stable clones that express the receptors at optimal levels, ... the time, cost, and labor of screening for clones that show optimal expression characteristics. ...

Using XL1-Red Mutator Strain to Generate Esterase Variants

... identify the desired variants from the numerous clones produced by random mutagenesis, we supplemented ... 750 colonies, several putative mutant clones were identified based on the red color that ... 9500 colonies were investigated. From these, 12 clones were selected, and the esterase was ...

A New Lambda Vector for Mammalian Expression

... insert DNA from lambda phage. When either single clones or the entire lambda library is converted to a ... and Kpn I restriction enzymes. All 12 clones contained a cDNA insert, ranging in ... can be easily screened, and individual clones can be quickly converted to the pBK-CMV vector ...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

... medium, hygromycin-resistant clones were isolated and tested with the PathDetect ... systems. Cell lysates from these stable clones were then prepared, assayed for ... rounds of selection with G418, we chose the clones that gave the best response when transfected ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

... 6 cfu/g of vector DNA. The percent of clones that contained inserts averaged 90%. ... cfu/g of vector DNA, and the percent of clones that contained inserts averaged 82%. ... efficiency and a higher percent of clones with inserts. Gel Isolation ...