cerevisiae in Biological Technology

Brewing better beer: Scientists determine the genomic origins of lager yeasts

... beers have been brewed with Saccharomyces cerevisiae (brewer's or baker's yeast). In contrast, lager ... yeast species, Saccharomyces bayanus and S. cerevisiae . It is thought that the contributions of both ... likely that each of these groups derived the S. cerevisiae portions of their genomes from distinct but ...

Sloning and the Kornberg group at Stanford University starting evaluation project regarding the synthesis of a Statistically Randomized Gene Library

... in Chemistry 2006) at Stanford University will perform in vivo screens based on the library provided by Sloning to identify relevant mutants of a S. cerevisiae RNA Polymerase II. Jan Van den Brulle, Scientific Director at Sloning commented, “We are looking forward to work together with Dr. ...

Growing old together: Yeast, worms and people may age by similar mechanisms

... led by Drs. Matt Kaeberlein and Brian Kennedy of the University of Washington conducted a genome-wide analysis of the yeast Saccharomyces cerevisiae and the nematode worm Caenorhabditis elegans , to identify genes that may regulate aging in humans. Nematodes and humans are more similar to each ...

Venter Institute Scientists Create First Synthetic Bacterial Genome

... the team found they could use homologous recombination (a process that cells use to repair damage to their chromosomes) in the yeast Saccharomyces cerevisiae to rapidly build the entire bacterial chromosome from large subassemblies. "This extraordinary accomplishment is a technological marvel that ...

Fermentation Cell Culture: University Style

... We primarily grow Saccharomyces cerevisiae in our laboratory, but we also grow ... complex purification. For S. cerevisiae and E. coli we perform preparative ... coli ), or multi-protein complexes ( S. cerevisiae ). For S. pombe we perform ...

Electroporation of Yeast Artificial Chromosomes

... the transformation of Saccharomyces cerevisiae with supercoiled plasmids. 3-6 In an attempt to ... investigated the electroporation of S. cerevisiae strain AB1380 (Mat a, ade2-1, can1-100, lys2-1, ... may improve on current results. 6 The S. cerevisiae strain used here (AB1380) is ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... transformation of Saccharomyces cerevisiae cells was optimized using the PDS-1000/He ... for nuclear transformation of S. cerevisiae cells (Heiser, 1992). The bombardment pressure ... for nuclear transformation of S. cerevisiae (Heiser, 1992). The spore density was ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

... reinhardtii, and Saccharomyces cerevisiae nuclear genomes, as well as C. reinhardtii chloroplasts and S. cerevisiae mitochondria was achieved by particle bombardment ... Nuclear transformation of S. cerevisiae is over ten-fold more efficient using 0.6 gold ...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

... Cauliflower The yeast auxotroph, S. cerevisiae st 948 (Armaleo, et al., 1990), contains ... on transformation of the uracil auxotroph S. cerevisiae st 948 with the uracil-containing ... Subsequent experiments with S. cerevisiae used a fractional factorial design to ...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

... profiles of wine strains of Saccharomyces cerevisiae were compared to those of a standard ... derived from laboratory strains of S. cerevisiae used in hybridizations with a distilling strain, ... complex industrial strains of S. cerevisiae provides useful qualitative data concerning ...

New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays

... test a wide range of data sets, including transcript groups of E.coli, S. cerevisiae and H. sapiens . In the human Latin Square, each transcript group was ... were in the 0.92-0.93 range for M. musculus, A. thaliana and S. cerevisiae , and 0.96-0.97 for D. melanogaster and E. coli . The ...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

... (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Paul Gavin # ... strains YRD15 (MATa, his3, ura3, leu2, p+) of the yeast S. cerevisiae was the parental strain used in this study. A gene cassette was ...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

... Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse ... strains YRD15 (MATa, his3, ura3, leu2, p+) of the yeast S. cerevisiae was the parental strain used in this study. Genes encoding the ...

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

... pombe (closed circles, lanes 4 and 6), Saccharomyces cerevisiae (open squares, lanes 3 and 5), lambda phage concatomers (open ... shown in Figure 2 were derived by running the S. pombe and S. cerevisiae chromosomes on 0.5%, 0.4%, 0.3% and 0.2% Chromosomal Grade ...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

... Sample material Saccharomyces cerevisiae was grown in YPD medium (50 g/l, rich) and SD base medium (26.7 ... amplification Total RNA from Saccharomyces cerevisiae grown in rich or minimal medium was labeled with Cy3 and Cy5 by three ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... era. Results of the yeast genome sequencing project indicate that S. cerevisiae shows homologous sequences for one-third of the human disease genes ... by plasmid shuffling. 3 Both the GAL1 and GAL10 promoters from S. cerevisiae are strictly regulated at the transcription level by the carbon source ...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

... specific-growth rate. Biotechnol. Bioeng., 33, 115-125 Aiba, S., S. Nagai and Y. Nishizaqa. 1976. Fed-batch culture of Saccharomyces cerevisiae : a perspective of computer control to enhance the productivity in bakers yeast cultivation. Biotechnol Bioeng., 18, 1001-1011 Bentley, ...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

... against Cell concentration (cells/mL). Figure 2: NucleoCounter YC-100 raw image of yeast cells in suspension ( Saccharomyces cerevisiae ) after Reagent YC-100 treatment using 100-fold dilution. Cell count is approx. 8 x 10 7 /mL. The objects have similar fluorescence intensities and ...

Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer

... Temperature probes Magnetic stirrer bars Yeast strains YRD15 (MATa, his3, ura3, leu2, rho+) of the yeast S. cerevisiae was the parental strain used in this study. The open reading frame encoding BFP was cloned into the yeast expression plasmid ...

Transformation of Filamentous Fungi by High-Voltage Electroporation

... into many fungal species in addition to T. harzianum, A. nidulans (Richey et al., 1989), F. solani (Richey et al., 1989), S. cerevisiae (Delorme, 1989; Hashimoto et al., 1985; Hill, 1989; Karube et al., 1985; Meilhoc et al., 1990; Rech et al., 1990; Weaver et ...

Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

... Leptosphaeria maculans Neurospora crassa Pichia pastoris Penicillium urticae Saccharomyces cerevisiae (6 strains) Schizosaccharomyces pombe Trichoderma harzianum Bacteria, gram negative ...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

... 1999). References Armaleo, D., et al., Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi, Curr. Genet., 17 , 97103 (1990) Bartels, R., Potato virus A, CMI/AAB Desc. Pl. Viruses , 54 (1971) Brunt, A. ...

Improve RNA Isolation for Array Analysis and More

... used at Ambion to isolate high quality RNA from Saccharomyces cerevisiae , Schizosaccharomyces pombe , Pichia pastoris , and Candida ... Total RNA Isolated Using the RiboPure-Yeast Kit. P. pastoris , S. cerevisiae RNA , C. albicans , and S. pombe RNA, isolated using the ...

Codon Bias-Adjusted BL21 Derivatives for Protein Expression

... 11.5 7.9 9.8 4.3 Saccharomyces cerevisiae 9.3 21.3 3.0 13.4 ...