capillaries

Intracytoplasmic Spermatocyte Injection (ICSI)

...rning the knob of the CellTram Air. Then the capillaries and the oocyte, (magnification x40) are shar...ocyte is released from the holding capillary. Both capillaries are moved to position 2 by pressing the joystick button. If several oocytes are obtaine...

Isolation of Microorganisms

...s to be isolated. For typical experiments, capillaries with an inner diameter of 5 m and a tip angle of 45 were used. These capillaries were designed in accordance with our specifications (Eppendorf). Dried bacteria cultu...

Transgenic Animals

... Femtotips, the standard capillaries for microinjection, are ideal for the injection of DNA, RNA, proteins and dyes into living cells. The shapes of the capillary tip ensures high cell viability and minimizes blockages by the injection material. The c...

CleanSEQ on the ABI 3100

...r and overlaid with mineral oil in the same capillaries for 99 consecutive runs. Formamide samples were run in parallel in alternate capillaries as a base line of capillary function. Samples loaded in formamide or in water overlaid...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

...he lesions or morphologically normal cells capillaries of 1025 m diameter were used. The section was overlaid with TE, pH 8.0 (10 mM Tris/HCl, 1 mM EDTA, pH 8.0). Aspiration only of the buffer served as contamination control. The aspirated cells/buffer were transferred to 5 l prot...

Oligonucleotide Purity Analysis by Capillary Electrophoresis

...on time and peak area. The analysis employs capillaries coated internally with a novel polymer (polyAAEE, acryloylaminoethoxyethanol) which ensures high-resolution separations over many runs. CE Oligonucleotide Kit Components The kit contains all the reagents necess...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

...put ABI PRISM 3100 Genetic Analyzer 16 capillaries for high throughput applications 654 samp...I PRISM 3100 Avant Genetic Analyzer 4 capillaries for low-to-medium throughput applications 163 samples in 24 hours Ease-of-use ...

Researchers report major advance in gene therapy technique

...tage of the access blood vessels have through the capillaries that sprout from them to tissue cells, Wolff says...njection forced the solution out of the veins into capillaries and then muscle tissue. The injections yielded substantial, stable levels of gene activity througho...