SoluLinK Introduces New One-Shot ChromaLink(TM) Biotin Antibody Labeling Kit with Built-in Biotin Quantification.
... kit features SoluLinK's
patented sulfo-ChromaLink biotin
linker, a water-soluble, biotin
reagent that allows rapid and direct, ... -- Built-in Quantitative Signal: Incorporated biotin
is quantified using a simple UV-scan -- no ...
SoluLinK Introduces ChromaLink(TM) Biotin Labeling Kit to Biotinylate and Measure With One Reagent
their newest product, the ChromaLink biotin
Labeling Kit, a biotinylation
reagent with a ... -- Built-in Quantitative Signal: Incorporated biotin
quantified by simple absorbance -- no HABA or ... products. SoluLinK's proprietary
Labeling Kit for biotinylation is one example. It ...
Assay Designs(TM), Inc. Announces Release of First Available Product for Multiplex Analysis of Heat Shock Proteins
... antibodies or antigen affinity purified polyclonal antibodies covalently coupled to latex beads. The detection antibodies are conjugated to biotin
followed by a streptavidin-PE conjugate and analyzed on a dual-laser flow cytometer. The HSP/Chaperone panel provides a sensitive, rapid and ...
SoluLinK Introduces NanoLink(TM) Streptavidin Magnetic Microspheres
magnetic microsphere. NanoLink(TM) Streptavidin particles are particularly
suited for high throughput robotic applications where high biotin
must be removed or immobilized without the presence of an iron leachate.
Features and Benefits include:
-- Highest free-biotin binding ...
Synthesize High Yields of Biotinylated aRNA
... a complete single round amplification kit for biotin
array analysis. The biotin
modified UTP and ratio of labeled
... Ambion has incorporated
labeling reagents into the MessageAmp II Kit to ...
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
... Sequential extraction procedures are being utilized
along with biotin
labeling to distinguish between cytoplasmic, membrane-associated,
Sequential Extraction and biotin
Labeling of Bacterial Proteins
Using the CSF-259-93 reference ...
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
... acid - 0.02 g/L
Cobalt chloride - 0.5 g/L
Zinc chloride - 20 g/L
Ferrous sulfate . 7 H 2 O - 65.0 g/L
- 0.2 g/L
Sulfuric acid - 5.0 ml
Water - to 1.0 liter
Glycerol Feed ...
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
... Sodium molybdate dihydrate . . . . . . . . . . . . . .0.2 g/L
Ferrous sulfate heptahydrate . . . . . . . . . . . . .. .65 g/L
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..0.2 g/L
6N sulfuric acid . . . . . . . . . . . . . . . . . . . . . . . .30 ...
A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
... following the Paradise protocol
and this material was taken into the
GeneChip IVT labeling Kit (Affymetrix).
labeled aRNA was quantitated
using the NanoDrop Spectrophotometer.
See Tables 2a and 2b for aRNA
9. 16 ...
High Throughput RNA Isolation for High Performance Microarray Analysis
... RNA, isolated from frozen mouse kidney
(1 g, each sample), was amplified using MessageAmp II-96 aRNA Amplification
Kit. The biotin
labeled amplified RNA was fragmented and hybridized to
an Affymetrix GeneChip Mouse Genome 430A 2.0 Array (total probe set=22,000).
Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
... with the MessageAmp II aRNA Kit (Ambion) using 20
ng of total RNA from each LCM sample. The resulting aRNA was labeled with
UTP and hybridized to an Affymetrix Mouse 230A GeneChip array.
(A) Graph summarizing Percent Present Call results for each sample.
Perform Microarray Analysis with Limited Bacterial RNA
... from it (MICROB Express Kit) was
used with the recommended Affymetrix labeling protocol or Ambions
MessageAmp II-Bacteria biotin
IVT labeling protocol. The data was
analyzed with Affymetrix Microarray Suite, Version 5 with scaling to all
probe sets and a ...
Improving RNA Amplification
... All RNA samples
were amplified and labeled with biotin-16-UTP/-11-CTP (PerkinElmer Life
Sciences), with the MessageAmp II Kit. biotin
labeled cRNA was fragmented,
then hybridized and stained using GeneChip Human Genome Focus Arrays following
the GeneChip ...
aRNA Synthesis and Labeling for Array Analysis
... can be used to
label amplified antisense RNA (aRNA) for array analysis. Cy
dyes, other fluorophores, or biotin
can be incorporated: 1)
during the in vitro transcription (IVT) step of aRNA synthesis
via modified-rNTP ...