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New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
...and provide an attractive method foruncovering the biological role of many gene products that cannot be studied inthe yeast. The mammalian two-hybrid system first developed by Dr. Dang andcolleagues 8 provides an excellent alternative to the yeast-basedsystem because this assay is performed in mammalian cells....Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
... for studying the molecular mechanisms underlying biological systems. Highly sensitive mass spectrometric techniques provide valuable peptide sequence information from very limited amounts of protein, which can be used to screen the wealth of information available in the NCBI databasesi...Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
...particularly when studying developmental and other biological processes forwhich the timing or dosage of gene ex...ate screening for inhibitors ofclinically relevant biological pathways and to explore potential applicationsfor gene therapy. 1 Most inducible mammalian systems...Epitope-Tagging Vectors for Functional Analysis in Yeast
... the epitope-tagged luciferase retains its biological activity, and the tags do not interfere with the enzyme activity. We consistently observed that the luciferase activities derived from the pESC-URA vector are approximately two- to three-fold less, compared to those derived fr...New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
...ications that are often required for their biological activity. This system also features the new pESP-2 vector ( figure 1 ), which retains all of the unique properties of the pESP-1 vector, 1 such as tight transcriptional regulation by thiamine and high expression level...High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
...a maltose-bindingprotein (MBP) tags] to affect the biological function of the protein ofinterest. CBP is efficiently translated in E. coli, and CBP fusionproteins are typically expressed to high levels. In addition, the CBP tag isefficiently phosphorylated by protein kinase A and thus, purified CBP fusionprotei...Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
...d IgG 1 , IgG 2a ,IgG 2b and IgG 3 . Because the biological functions and biochemical characteristics of classesand isotypes differ, distinguishing the classes and isotypes of animmunoglobulin molecule is critical. Knowledge of antibody structure directs thestrategies for purifying immunoglobulins. For exampl...Yeast Protein Production System Features High Yields and One-Step Purification
.... Our functional assays indicated that the biological activities of the GST-calmodulin fusion protein and the GST-MEK1 fusion protein expressed in S. pombe were retained (data not shown). However, if desired, the GST tag can be removed by treating the purified fusion protein with...Using XL1-Red Mutator Strain to Generate Esterase Variants
...lones, a new class ofmacrolides showing taxol-like biological activity. 9 Mutation and Screening Figure 2 In Figure 2 , plasmids isolated from E. coli colonies produced during mutation cycles using XL1-Red competent cells were transferred in...Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
...oprotein carbohydrates play important roles in the biological processesof glycosylated proteins. 1 However, analyzing glycan moieties can bean arduous process, given the small recovery amounts, impurity, and lowmolecular mass of released glycans. In a recent advance, gel electrophoresis hasbeen used to separat...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
...ate screening for inhibitors of clinicallyrelevant biological pathways, and potential applications for gene therapy arebeing explored. 1 Stratagenes complete control inducible system is based on the insect molting hormone ecdysone, which can stimulatetranscriptional activation in mammalian cells harboring ...Highly Active and Removable AffinityTagged Lambda Phosphatase
...tein kinase. Protein phosphorylation governs many biological processes, includingmetabolism, gene expression, cytoskeletal architecture, cell adhesion and thecell cycle. 1 The level of protein phosphorylation in cells ismodulated by two large families of enzymes, the protein kinases and the proteinphosphatase...RT-PCR Primers for the Study of Apoptosis
...li Stratagene The apoptotic response is a complex biological process that requires theregulation of a variety of genes that interact with and respond to intracellularand extracellular stimuli. Stratagene is introducing RT-PCR primer sets that are specific for genes involved in inducing and regulating theapop...Mammalian and Bacterial Expression in One Vector
...iring a significant investment of time and biological reagents. Stratagene has now eliminated the need to subclone from one vector system to another by combining the essential features of both in a dual expression vector. The novel pDual vector ( figure 1 ) can achieve high-level...New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
...expression of many genes and elicit a widerange of biological functions, including tissue repair, activation of variousblood cells involved in antibody production, inflammation, and host defenseagainst viruses. 2,3 IFNs have also been used as therapeutic agentsagainst many viral diseases, including acquired im...Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
... DNA into mammalian cells is vital tomany areas of biological research. The ability to study the effect ofintroducing a gene or oligonucleotide of interest in a cell line may depend onthe effectiveness of the transfection reagent. Stratagenes LipoTAXItransfection reagent can be used to achieve efficient transfe...Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
... more challenging step will be to characterize the biological role of eachgene and the way in which the encoded ...tures and requires theformation of a homodimer for biological activity. Results of the enzymatic GFPassays demonstrate that GFP tagged with c-myc and HIS6 was bio...High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
...llows studies in the more physiologically relevant biological models.Immortalized or transformed cell lines often have undefined phenotypes, andthese phenotypes must be extensively characterized. We transfected the pCMVbgal reporter plasmid into HUVEC and HUAEC cells, which are primary cells, wit...New PCR Primers for Human Diseases
.... To aid in the diagnosis of specific diseases in biological materials,Stratagene is introducing a series of PCR primers that are derived fromsequences published in GenBank. These human disease primers amplify genes fromsamples infected with the following organisms: Human immunodeficiency virus,type 1 (HIV-1),...A membrane-lid tube for bacteria cultivation and freeze-drying
...of standard protocols. It is possible to dry biological samples within one working day or overnight. The main advantage is the sterile condition achieved by the bacteria-proof membranelid when Lid Bac is used. Conversely, when open tubes are used, the chemical sterilization of the dryin...A Unique Dimension in Biological Purity
... for metabolic activities or the presence of biological material. DNA and RNase Contaminating nucleic acids and enzymes that cleave DNA or RNA are the most critical substances that can very much influence experimental work in molecular biology, eg, the Polym...Principles and applications of electrical cell fusion
... J.,Scheurich, P., Electric field induced fusionof biological cells. Biophys. Struct. Mech.,1980, 6 (Suppl.), 86. [3] Zimmermann, U., Vienken, J., Pilwat,G., Development of drug carrier systems:electrical field induced effects in cellmembranes. Bioelectrochem. Bioenerg.,1980, 7, 553. [4] Khler. G., Milst...Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
...searcher to isolate gDNA from a variety of biological samples, including buffy coat. The purification method is based on Proteinase K lysis followed by collection of the crude high molecular weight gDNA on a membrane. The gDNA is then washed to remove contaminants and eluted in a...EppendorfPerfect gDNA Blood Mini Kit
...l tool for the detection of C. burnetii in biological samples 6. However, this necessitates the extraction of the genomic DNA of C. burnetii without purification of bacteria. Thus, the samples contain gDNA of the bacteria and the eukaryotic cells. Different methods are reported i...Principles and applications of electrical cell fusion
... J.,Scheurich, P., Electric field induced fusionof biological cells. Biophys. Struct. Mech.,1980, 6 (Suppl.), 86. [3] Zimmermann, U., Vienken, J., Pilwat,G., Development of drug carrier systems:electrical field induced effects in cellmembranes. Bioelectrochem. Bioenerg.,1980, 7, 553. [4] Khler. G., Milst...EppendorfPerfect gDNA Blood Mini Kit
...l tool for the detection of C. burnetii in biological samples 6. However, this necessitates the extraction of the genomic DNA of C. burnetii without purification of bacteria. Thus, the samples contain gDNA of the bacteria and the eukaryotic cells. Different methods are reported i...Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
...searcher to isolate gDNA from a variety of biological samples, including buffy coat. The purification method is based on Proteinase K lysis followed by collection of the crude high molecular weight gDNA on a membrane. The gDNA is then washed to remove contaminants and eluted in a......his technology field focuses on the development of biological substituents that maintain, regenerate and...ages occur in phase I of the liver metabolism. The biological function of these reactions is the transformation of toxic substances or external ma......bed (1). The technique referenced utilized biological and chemical components to orchestrate an enzymatic amplification reaction conducted by a DNA polymerase. This technique was named the Polymerase Chain Reaction (PCR). This article describes an important extension of the PCR t......arch on the role of certain genes in complex biological processes. Modified genomic loci can be easily distinguished by multiplex PCR. To distinguish the wild-type from the mutant gene locus, pairs of primers should be designed that are specific either for the wild-type locus or for the ...Critical factors for successful microarray and real-time PCR analyses
...et genes that are differentially expressed between biological samples, and quantitative real-time RT-PCR t... preservation of the RNA expression profile in the biological sample as well as the efficiency of cDNA labeling and cleanup. Newly developed reagents ...RNeasy Protect Mini, Midi, and Maxi Kits
...eagent quickly permeates tissues and other biological materials,* stabilizing and protecting the... RNeasy procedure. * Can be used with other biological materials, such as eukaryotic cell-culture cells or white blood cells Stabilization......on to methionine sulfoxide (MetSO) in vivo 1 . The biological consequences of this oxidation can vary depending on the specific site of the oxidation: MetSO at the Nterminal end of CaM had an effect on CaM-dependent nitric oxide synthase (type-I) whereas MetSO near the C-terminus affected ......roperties, it must be routinely monitored in biological samples by veterinary and human doping contr...he unequivocal identification of analytes in biological samples due to endogenous matrix interferents. This report describes the use of h...... at the low sample concentrations typical in biological matrices. The wide range of possible modifications further complicates data interpretation. With inherent high sensitivity, selectivity, and sophisticated Data Dependent operational capabilities, the Thermo Finnigan LCQ Series of io......ion for highthroughput quantitation of analytes in biological matrices. Paroxetine spiked into bovine plasma was injected as an intra-day batch consisting of blanks, calibration standards, and QCs at eight replicates. TSQ Quantum Tune View Optimization of Paroxetine ...Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
...at are copies of nucleic acids derived fromvarious biological sources. This requires reliable and efficient tools for preparationand labeling of targets: RNA needs to be extracted fromsmall amounts of precious sample materials, and labeledeither directly during cDNA pre......lysis ofgenes, and it has been used extensively in biological andmedical research for more than 20 years. To allow thesimultaneous analysis of large numbers of genes by insitu hybridization, the procedure was automated fairlyrecently. Automation of nonradioactive in situ hybridization notonly ...Use of Transcriptor Reverse Transcriptase in Microarray Analysis
...ion is rapidlybecoming a common tool for molecular biological investigations.The ability to simultaneously monitor tens ofthousands of genes affords an unprecedented view ofthe transcriptional changes that underlie cellularprocesses. Microarrays are ideal for genetic investigationsof intracellular pathogens suc...... which target preparation methods would reflectthe biological situation best, an independent method(quantitative RT-PCR) was applied to a number of genes.We randomly selected two genes from a group of upregulatedgenes and two genes from a group of downregulatedgenes in rich vs. minimal medium. We alsoselected t...Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
...t the quality of the signal depends on both biological and technical factors (based mainly upon the design considerations used in the array scanner). For instance, maximizing the collection of the emitted photons (N) is dependent upon designing efficient pathways not only fo......ime and money, allowing the researcher to focus on biological questions. An example of one type of experiment that is possible with the Silencer Validated siRNAs is described in " Using Validated siRNAs in Functional Genomics Assays ". Of course, many other applications exist, in......ower than bovine pancreas. Safety of biological products is also an emerging issue. Eliminating animal tissues and animal-derived components from the production process prevents potential transmission of pathogens. Materials derived from bovine sources are of special concer...Ambion Offers Gene Expression Profiling Service
... Setting up an experimental design that includes biological and analytical perspectives Replication for efficient analysis Sampling Selection of controls Cost efficacy: fewest arrays for the most information Recommending statistical a......or identifying genes involved in biological pathways. This article analyzes the effectiveness of two strategies--transfection of individual siRNAs and transfection of pools of siRNAs--for large-scale screening experiments. The increased eff...Automated RNA Amplification for Arrays
...nique to profile gene expression in multiple biological samples. Ambion's collaboration with GeneXP Biosciences, Inc. has resulted in the integration of a high throughput version of Ambion's Amino Allyl MessageAmp aRNA Amplification protocol with GeneXP's BioGridArray (BGA), producing a ...Designing Controls for siRNA Experiments
...l for analyzing gene function, elucidating biological pathways, and identifying and validating potential drug targets. The RNAi field is still in its infancy, and recommendations on experimental design and proper controls are likely to evolve. Ambion is committed to bringing rese...Increase Protein Activity and Yield
...h eukaryotic protein translation Higher biological activity than other E. coli lysate s... ability to synthesize protein with higher biological activity from both prokaryotic and eukaryotic transcripts. Now, more recent data shows Activ...miRNA Expression in White Blood Cells
...A, and rRNAs) from virtually any biological sample. The patented mir Vana m... is increasing. Further investigation of the biological role of miRNA will be facilitated by methods such as those incor...Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
...iments is to assign gene function, analyze biological pathways, or validate potential drug targets. But ... conducted to study gene function, analyze biological pathways, or validate drug targets, optimized siRNA delivery conditions ...Maximize Viral RNA Yield from Biological Fluid
...zed for isolating viral RNA from biological fluids and cell-free samples such as serum, plasma...s RNA isolation product to obtain RNA from biological fluids for their validated viral testing process. When they compared this validated system t...Isolation to Functional Analyses
...uggest that these molecules play key roles in many biological processes, including differentiation and oncogenesis. Functional studies featuring reagents for over- and under-expressing miRNAs will then enable a better understanding of miRNA...Novel MicroRNA Array Technology for Sensitive miRNA Profiling
... differentially expressed in virtually any biological sample. Figure 1. miRNA Array Procedure. (A) miRNA are purified from tissue or cell samples (e.g. using Ambion's mir Vana miRNA Isolation Kit and an electrophoresis unit specialized for small RNA purificati...Isolate Total RNA and Protein From the Same Sample
...e isolated from replicates of two types of biological samples, and various quantitative and qualitative parameters were measured. The experimental samples tested were human cell lines assayed 2 days after plating (12 replicates, Figure 2), and mouse liver tissue kept frozen for 2...Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
...g increasingly important for understanding biological processes. While the <A href="http://www.ambion.com/catalog/CatNum.php?1931">RNAqueous-Micro Kit has always been ideal for isolating total RNA from microscale samples ( 5 cultured cells and <10 mg tissu...Enhanced siRNA Delivery and Long-term Gene Silencing
...to HeLa cells. They could also be due to a biological difference between the two cell types; e.g. their ability to overcome or adapt to reduced cyclophilin expression. Additional experiments using cloned populations of infected cells expressing different levels of siRNA will be r...High Throughput siRNA Electroporation
...nt error, and assay error, as well as true biological variability. Figure 2. Reduction of GAPDH Gene Expression in NHDF-neo Cells. siRNA (1 g) was electroporated into NHDF-neo (normal numan dermal fibroblast cells, an adherent primary cell type) using the siPOR...Highly Sensitive microRNA Array Performance
...procedure as well as a confirmation of the biological significance of miRNAs in adult human tissues involved profiling miRNA expression in biological samples. We isolated miRNA from 26 normal human tissues. Half of each sample was used to cre......ies in identifying the genes and biological pathways they regulate. One begi...an monitor changes in a specific biological process in response to up- or down-regulation of miRNA activity. Depending on pre-......relatively high concentrations in many biological materials. For example, the pancreas is rich in RNase (>1 mg RNase/1 g tissue) and is the source for most commercially produced RNase A (and its glycosylated derivative, RNase B). RNase A is an en...Other ContentsherniatedherniatedgenitalgenitalgenitalgenitalgenitallabialissimplexsimplexsimplexsimplexsimplexsimplexherpesvirusherpesvirushistaminehistaminehistamineOther TagsPolishPolishActivistsForcepsEarthquakeAirwayAirway
(Date:9/4/2008)...use University and the Serum Institute of India co...for children living in some of the most impoverish...rded $250,000 to a team of SU researchers led by R...he College of Arts and Sciences, to develop new or...e form of diarrhea that affects infants and young ...
(Date:9/4/2008)...ems are constantly evolving in ways that increase ...uctuations and internal errors. Now, in a study of...ch team has found new evidence that evolution has ... suited to handle potentially harmful changes like...published online this week in the journal PNAS , ...
(Date:9/4/2008)...ize the tuna population? Can we compute how to tra...production? , Those and other questions will be ...ians, economists, biologists and environmental sci...nstitute for Computational Sustainability, being l... Science Foundation (NSF). This program is designe...
(Date:9/4/2008)...ogy company have joined forces to develop a world-...to predict premature birth with greater accuracy.,...ewcastle and Symbion Pathology are combining,expe...gy to develop a computer program to predict,women...term births occur in Australia each year. Prematur...
Breaking Biology News(10 mins):Syracuse University partners with Serum Institute of India to develop vaccines for children 2Syracuse University partners with Serum Institute of India to develop vaccines for children 3Syracuse University partners with Serum Institute of India to develop vaccines for children 4New evidence on the robustness of metabolic networks 2Cornell gets $10 million NSF grant to establish new sustainability institute 2World-first to predict premature births 2Solutions for Parents Who Need Cold Remedies for Their Children 9795 1Solutions for Parents Who Need Cold Remedies for Their Children 9795 2Biophysical Society to host discussions on the teaching of evolution and on the energy crisis 1966 1Biophysical Society to host discussions on the teaching of evolution and on the energy crisis 1966 2National study will look at German made heart pump for children needing heart transplants 9791 1National study will look at German made heart pump for children needing heart transplants 9791 2National study will look at German made heart pump for children needing heart transplants 9791 3Gift of Life Celebrates Saving Lives and Setting Records in 2007 9788 1Gift of Life Celebrates Saving Lives and Setting Records in 2007 9788 2Gift of Life Celebrates Saving Lives and Setting Records in 2007 9788 3(Date:9/4/2008)... may be shortchanging their golden years by tappin...iversity of Illinois expert who has studied the fe..., More than three of every four Americans trim th...iming benefits early, a choice many might forego i... consequences, law professor Richard L. Kaplan say...
(Date:9/4/2008)...ncology contract research organization, has publis...uanced Approach to Get Into the Clinic Faster.&quo...al research scientist with 35 years of experience,...mmonly viewed as straightforward investigations ru...rue integration with preclinical, regulatory and c...
(Date:9/4/2008)...NORWICH, England and ONGAR, Ireland,Sept. 4 /PRNe...ofield Media,Europe Ltd, announced on the 29th Aug... publication from Silverdog Publishing., Health a... the iconic,Health and Safety at Work Act 1974 and.... It has been relied upon by blue-chip corporates,...
(Date:9/4/2008)...-FirstCall/ -- ULURU Inc. (Amex:,ULU) today announ...Weisel,Partners Healthcare Conference at 3:15 p.m....out ULURU Inc.:, ULURU Inc. is an emerging specia...ent of a portfolio of wound management, plastic su...nd consumers improved clinical,outcomes through co...
Breaking Medicine News(10 mins):Health News:New guide explores making the most of Social Security 2Health News:New guide explores making the most of Social Security 3Health News:Oncology Clinical Research Scientist Recommends a Nuanced Preclinical Study Process to Speed Drug Development 2Health News:Oncology Clinical Research Scientist Recommends a Nuanced Preclinical Study Process to Speed Drug Development 3Health News:Schofield Publishing Enters the Newsletter Market 2[0] New guide explores making the most of Social Security[0] Oncology Clinical Research Scientist Recommends a Nuanced Preclinical Study Process to Speed Drug Development [0] Schofield Publishing Enters the Newsletter Market[0] ULURU Inc. to Present at the Thomas Weisel Partners Healthcare Conference 2008
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