Bacteriophage in Biological Technology

SANYO Donates Air Purification Systems Effective Against Flu Viruses to New York Airports and Schools

... reducing the level of viable airborne viruses, according to an independent research institution in the US. This research study included tests using bacteriophage MS2, commonly used by the scientific and testing community as a representative virus, which roughly approximates the aerosol-related physical ...

MIT, BU team combats antibiotic resistance with engineered viruses

... beta-lactams and aminoglyclosides) and had good results with all three. In mice infected with bacteria, those treated with both engineered bacteriophage and antibiotics had an 80 percent survival rate, compared with 50 percent for mice treated with natural bacteriophages and antibiotics, 20 percent ...

J. Craig Venter Institute Researchers Publish Significant Advance in Genome Assembly Technology

... Cynthia Andrews-Pfannkoch) made the first significant strides in the development of a synthetic genome by assembling the 5,386 base pair genome of bacteriophage phi X174 (phi X). They did so using short, single strands of synthetically produced, commercially available DNA (known as oligonucleotides) and using ...

MicroPhage Closes Latest Financing

... against current methods. We are looking forward to completing our validation work and launching products, which leverage the tremendous power of bacteriophage amplification technology for rapid bacterial identification and susceptibility testing." About MicroPhage Based in Longmont, Colo. ...

Collegiate Inventors Competition(R) Recognizes Top Student Inventors

... and Massachusetts Institute of Technology (Advisor: J.J. Collins) Combating Antibiotic-resistant Bacteria and Bacterial Biofilms with Engineered bacteriophage and Synthetic Gene Sensors - An engineered bacteriophage - a virus that infects bacteria-that works in conjunction with antibiotics, making them much ...

Strategic Diagnostics Reports Fourth Quarter and Full-Year Financial Results

... with the Company's new products and services utilizing the GAT(TM) platform as well as ongoing development of the ethanol application of its bacteriophage technology for the selective control of lactic acid-producing bacteria. Pre-tax income for the fourth quarter of 2007 totaled $409,000, compared to ...

Venter Institute Scientists Create First Synthetic Bacterial Genome

... Smith and Hutchison made the first significant strides in the development of a synthetic genome by their work in assembling the 5,386 base pair bacteriophage FX174 (phi X). They did so using short, single strands of synthetically produced, commercially available DNA (known as oligonucleotides) and using an ...

Tips from the Journals of the American Society for Microbiology

... Phage Therapy May Control Staph Infections in Humans Including MRSA Researchers from Italy have identified a bacteriophage active against Staphylococcus aureus, including methicillin-resistant strains, in mice and possibly humans. They report their findings in the August ...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

... including human, mouse, yeast, and bacteriophage total RNA or poly(A) + -selected mRNA. ... of each), RNA (200 ng of total RNA or 1 ng of bacteriophage MS2 RNA), 1.25 U of MMLV-RT, and 2.5 U of ... and RNA (200 ng of total RNA or 1 ng of bacteriophage MS2 RNA). The reaction mixtures were incubated ...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... Science, 236 , 806812 (1987). 2. Sternberg, N., bacteriophage P1 cloning system for the isolation, amplification, and ... and Botstein, D., Propagation of some human DNA sequences in bacteriophage 1 vectors requires mutant Escherichia coli hosts, Proc. Natl. ...

Ambion Custom

... Figure 1. Crystals of Group I Intron From bacteriophage Twort. Today's laboratory demands often require ... quantities of RNA--3.75 mg purified group I intron from bacteriophage Twort--for crystallography (Figure 1). The superior performance ...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

... polio, measles, mumps, and rubella vaccines. bacteriophage A virus that infects bacteria, sometimes used as a vector. base ... artificially constructed plasmid vector that contains a specific bacteriophage gene, which allows it to carry up to 45,000 base pairs of ...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

... of source DNA is a basic building block technique in molecular biology research. The TempliPhi DNA Sequencing Template Amplification Kit employs bacteriophage j29 DNA polymerase applied in an isothermal rolling circle amplification (RCA) 1 to produce large amounts of product DNA (1-3 μg) 2 and ...

The Bio-Rad Silver Stain, Rev E

... Proc Natl Acad Sci USA 82, 57565760 (1985) 39. Laemmli UK, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680685 (1970) 40. Lee-Huang S, Cloning and expression of human erythropoietin cDNA in Escherichia coli, Proc ...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... Anal. NY Acad. Sci., 121 , 321 (1964). 31. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T, Nature, 680686 (1970). 32. Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of protein from polyacrylamide gels ...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

... style='mso-char-type:symbol; mso-symbol-font-family:Symbol'>l bacteriophage DNA standard. The data shown in this application note were generated using this standard, prepared in serial dilutions. ...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... formation of the desired cloning vector. Materials And Methods The bacteriophage fd-tet which confers tetracycline resistance upon infected cells was donated by Dr. John McCafferty (Cambridge Antibody Technology). ...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

... References: Barnes, W.M. (1994) PCR amplification of up to 35 kb DNA with high fidelity and high yield from l bacteriophage templates. Proc. Natl. Acad. Sci. USA. 91: 2216-2220. Cantor, C.R. and C.L. Smith (1999) Genomics. John Wiley & Sons, ...

The TripleMaster PCR System for three different amplifications

... Literature [1] Barnes, W. M.; PCR amplification of up to 35 kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. USA. 1994 Mar 15; 91 (6): 2216-2220. [2] Cheng, S., Kolmodin, L.A.; XL PCR ...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

... in purified DNA. The Perfectprep Gel Cleanup kit was used to purify a 3.49 kb PCR1 fragment amplified from bacteriophage Lambda. The purified DNA was subjected to restriction enzyme digestion using EcoRI, EcoRV and HindIII. The digests were done in ...

Purified Single-Stranded M13 DNA for Automated Sequencing

... DNA purification kit allows easy, quick, and reliable purification of bacteriophage single-stranded DNA. The yield of single-stranded DNA recovered from ... StrataPrep M13 method of Phagescript lysate (a modified M13 bacteriophage 8 ), was at least twice that obtained from the SGB method ( ...