apparatus

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

...nutes ( Figure 1 ). The gel apparatus and precast gel are easily assembled in four steps...gel assembly is complete. Finally, install the gel apparatus on the LI-COR instrument and carry out a 20 minute pre-electrophoresis prior to load...

TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates

...ng the BIO-RAD Pulse Field Electrophoresis apparatus (BIO-RAD, Hercules, CA, USA). A Low Range PFG Marker (NEB, Beverly, MA, USA) and Lambda Hind III Marker (Worthington, Lakewood, NJ, USA) were run with the samples for size determination. 250 ng of gDNA template were loaded ont...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

...ng routine DNA synthesis determinationspossess the apparatus required for luminescencemeasurements because it is considerablymore expensive than systems for measuring absorption.The experiments described here may help in thisdecision-making process....

Ultra-Fast Purification of Small Nucleic Acids

...gn and mechanical circuitry, the apparatus is designed to be an easy addition to any lab. This instrument is provided with a power cord containing standard banana plugs at one end--thus permitting use with most common laboratory power supp...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

...9 with HCl) in the lower and elution chambers. The apparatus was operated at 4 watts constant power until the dye front migrated to the bottom of the tube gel. At this time, the tube gel was connected to a peristaltic pump (flowing at a rate of 0.1 ml/min), and 1 ml fractions were isola...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

...ditions were 130 V for 6 hours on the DCode apparatus (Bio-Rad). Gels were stained in 1 g/ml ethidium bromide and visualized by UV transillumination. The point mutations were confirmed by sequencing the PCR amplified fragments with the non-GC clamped primer of each exon, using...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... A single pulse was delivered (Gene Pulser II apparatus used with the Pulse Controller II) at 25 F, 2.50 kV and 200 ohms (time constant=4.54.7 msec). Cells were immediately resuspended in 1 ml of SOC (room temperature) and incubated at 37 C for 45 minutes. Dilutions were plated on ...

Electrotransformation of E. coli With M13 DNA

...olume. Mixtures were pulsed with the Gene Pulser apparatus and Pulse Controller at various voltages with 25 F and 200 Ω in chilled 0.1 cm electroporation cuvettes (Bio-Rad); immediately after pulsing, 200 l of an overnight or late logphase culture of XL1-Blue cells were a...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...ctro-Competent E. coli cells. The E. coli Pulser apparatus (Bio-Rad) was set to a voltage of 1.80 kV, and chilled 0.1 cm electrode gap cuvettes were used. Time constants of 4.7 - 4.8 msec were observed. Within 10 seconds of the pulse, 1 ml of SOC medium (2% Bacto tryptone, 0.5...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

...ative comb using the Mini-PROTEAN II gel apparatus (Bio-Rad). Sodium dodecyl sulfate (SDS) wa...e Whole Gel Eluter, this laboratory feels that the apparatus has greatly facilitated the isolation of a highly purified form of the DNA synthesome, ...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

... Gel Electrophoresis The electrophoretic apparatus (CHEF-DR II cell, Bio-Rad Laboratories) has been described. 6 Standard conditions were a 30- hour run (23 hours with a 60 second switching interval followed by 7 hours with 100 second intervals) at 200 volts, 1.8 liters of ...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

... of living cells using the Helios gene gun apparatus and the detection of repoter gene activity after particle bombardment under in vitro and in vivo conditions. Materials and Methods CELL CULTURE AND ANIMALS HeLa cells were cultured in minimal essenti...

Electroporation of Primary Bone Marrow Cells

...ng electroporation. We have used the Gene Pulser apparatus to electro-transfect either a murine MHC c... We have also used the Gene Pulser apparatus to stably transfect adherent 929 cells with the dhfr gene in order to compare the conditions...

Electroporation of Yeast Artificial Chromosomes

....1 g) just before electroporation. A Gene Pulser apparatus connected to the Pulse Controller accessor...r voltage experiments with the Gene Pulser apparatus (which generates an exponential decay pulse) have a less disruptive effect on large DNA mole...

Transformation of Filamentous Fungi by High-Voltage Electroporation

... (Bio-Rad Laboratories) by using a Gene Pulser apparatus with the Pulse Controller (Bio-Rad Laboratories). Following delivery of the electrical pulse, OSC were mixed with 1.0 ml of Potato Dextrose Broth (PDB) plus 1.2 M sorbitol (PDBS), incubated for 10 minutes on ice, and then for ...

Transgenic Zebrafish by Electroporation

... consuming. We have used the Bio-Rad Gene Pulser apparatus to generate transgenic fish by electropora...imes using the 0.25 F capacitor of the Gene Pulser apparatus (Bio-Rad). The pulse field strength is 125 V/cm, and each time constant is approximately ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...ls. These travel gels were run in the DCode system apparatus at a constant temperature of 56 C and 200 V for a total of 5 hours to determine the optimal running time. This was found to be 3 h for the apoA-I fragments and 4h for apoB. The gels were stained for 30 minutes in SYBR Green ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...JECTION OF TRACERS ◊ Stereotaxic apparatus for the animal subject (varies depending on specie..., 1-5 uL Cat. #5-000-1001) ◊ Microinjection apparatus (Narishige PE-2) II. FREEZING BRAIN AND CRYOSECTIONING ◊ Tissue embedding molds (...

Practical Applications of HyperDSC in a Pharmaceutical Laboratory

...ginning at -65 C and carried out at 200 C/min, the apparatus reaches equilibrium at about 30 C, the temperature from which any transition can be measured. By comparison, a classical heat-flux DSC can take 30 to 60 seconds and is not ready for the detection of a transition before +35 C or more likely +135 C, wh...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

...NTRODUCTION Calibration of a dissolution apparatus is necessary to produce accurate results for disso...ity Test in USP Dissolution. 1 Each individual apparatus (i.e. spindle or basket) must be tested with disintegrating type and non-disintegrating type USP dis...

Intellectual Property: Are you an infringer?

...ture, combination or composition, or a material or apparatus for use in practicing a patented process, constituting a material part of the invention, knowing the same to be especially made or especially adapted for use in an infringement of such patent, and not a staple article or commodity of commerce suitabl...

LEDS Bring Relief to Young Cancer Patients

...of bone marrow transplant patients. Use of the LED apparatus has advanced to the second phase of clinical trials in U.S. and foreign hospitals. Results from the first round of tests were encouraging, prompting researchers to expand the trials as they seek approval for the treatment as a standard of care for or...

Oshkoshs Pierce Security Vehicle Ranked in top 100 Technological Innovations for 2003

...ruck Corporation is a manufacturer of custom fire apparatus and homeland security vehicles in North America. Among the company's other homeland security vehicles are specialized HAZMAT and bomb response units, urban search and rescue vehicles and decontamination trucks. "It is an incredible honor for Pierce...