Antiserum in Biological Technology

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

... in vivo, we performed ChIP assays using Pdx1 antiserum and chromatin from Pdx1-transfected ... sample immunoprecipitated with Pdx1 antiserum from the ΔC T value for the corresponding ... that has been immunoprecipitated using Pdx1 antiserum reaches the threshold approximately ...

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

... in four of six mice (detected at a 1:10 antiserum dilution) following two intramuscular ... response to the HBsAg (detected at a 1:10 antiserum dilution), with one of these responses ... antibody responses, detectable at an antiserum dilution of 1:10,000 for two mice, 1:1,000 ...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... were also characterized by polyclonal antiserum to βLG (Nordic Immunologic, Tilburg, ... was incubated with the polyclonal antiserum to βLG (5 l/50 ml PBS/0.05% Tween 20) for 2 ... horseradish peroxidase-coated second antiserum (10 l/50 ml PBS Tween 20 (0.05%) for 2 h at RT ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... were confirmed by immunoblotting using porcine hyperimmune antiserum to TGEV (Stott, 1989) or a TGEV neutralizing monoclonal antibody ... blotting of the purified proteins with porcine hyperimmune antiserum to TGEV (S, M, N) in western blot and the ELISA reaction with ...

Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

... a centered well. A sample is added in the central well and isotyping antiserum is added in the peripheral wells. As the sample and isotyping antibodies ... The precipitation line that occurs between the sample well and an antiserum well indicates the isotype. This assay is inexpensive and simple to use. ...

Immunoassay of Theophylline by fluorescence polarisation

...

A pre-treatment solution, the theophylline standard in a pH-7.2 buffer, the theophylline-fluorescein tracer, and antiserum were mixed together and incubated for three minutes before determining the fluorescence polarization. The theophylline samples were from General ...

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

... a sodium acetate buffer and pipetted manually, in duplicate, into LP4 tubes. The remaining components (i.e., standard cAMP [ 125 I] tracer and cAMP antiserum complex) were also added manually. After overnight incubation, binding protein was added to all tubes (except totals). Following mixing, the samples ...

Mammalian and Bacterial Expression in One Vector

... CBP-tagged GFP fusion protein in bacterial and mammalian cell lysates was further verified by Western blot analyses using GFP antiserum ( Figures 2C ) and the Affinity CBP fusion detection kit (data not shown). ...