Ambiguous in Biological Technology

Making deals with evolving technologies in mind

... 1991 settlement agreement. Litigation resulted in the United Kingdom from ambiguous language regarding evolving technology that, as evident by convincing ... One drafting rule to take away from this case would be to avoid using ambiguous phrases such as on or in connection with. This phrase created problems for ...

UN committee opposes human cloning

... application of genetic engineering techniques that may be contrary to human dignity." However, because it is a non-binding resolution loaded with ambiguous language about what is being banned, the proposal is for practical purposes meaningless, according to University of Wisconsin-Madison law and medical ...

The end of technology history

... play, and shop? Well, why not? Yeah, I know. There's security, privacy, and the unwillingness of retailers to invest in new devices, and ambiguous business models, and cost, and everything else. But we're not talking about technology anymore, are we? Funny thing about technology assimilation. It ...

An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis

... In the small molecule screening phase of drug discovery, animal experiments are low throughput and often technically too challenging, expensive and ambiguous to be used. Scoring the degree of blood vessel formation using an unbiased microscope approach allows this assay to be used in secondary screening ...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

... form factor (nuclear symmetry) settings to segregate mono-nucleate and bi-nucleate cells. Use delta threshold settings if required to assign ambiguous cells as unclassified.

4. Adjust threshold in the green (FITC) channel to identify cell boundaries.

5. ...

Aurum Plasmid 96 Kit Tech Note, Rev A

... were sequenced on an Applera ABI PRISM 3100 capillary array sequencer, and the resulting electropherograms were analyzed for ambiguous counts within the first 600 bases to assess relative comparative read lengths. Results Plasmid Yields ...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

... and the large number of species that may be encountered. The morphological and physiological traits of most microbes are ambiguous and provide few clues as to their identity; nucleic acid sequence comparison is the most fundamental way to classify ...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

... be analyzed (100 or more). Restriction fragment pattern analysis is far simpler; however with mixtures this method yields highly ambiguous results due to spurious matches. These ambiguities are prevented if the PCR products are initially separated by using TTGE. ...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

... runs 160 fish per day on DGGE (8 gels) using two DCode systems (80 fish each for daytime (A1 locus) and nighttime (A2locus) runs). ambiguous scoring of shifting alleles necessitates the re-running of less than 2% of all fish analyzed. This rapid rate of analysis of ...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

... similar results in terms of RNA yield and quality (average yield: approx. 1600 pg; slightly degraded RNA). In the case of MB staining, ambiguous results were observed (average yield: approx. 730 pg; partially degraded RNA; figure 3D). The 28 S band is almost entirely missing, ...

Structural Determination of Flavonoids Using MSn

... isolated before a subsequent MS/MS experiment is performed. Due to this isolation step, fragmentation spectra are generally less complex and ambiguous than traditional MS/MS spectra. Through successive MS/MS steps, product origins can be assigned. As the following experiments will ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

... or a silica-gel membrane method. Both sequences were aligned with a published data set. For the silica-gel membrane method, 12 ambiguous base calls were identified in 801 bases of sequence. Only 8 ambiguities were seen in 795 bases of sequence from plasmid that ...