Greater Amplification Specificity with New Hot Start PCR Enzyme
... step 4,5
or withheld by a physical wax
barrier until temperatures sufficient to
are achieved. 6
... awkward manipulations
associated with wax
barrier or manual methods.
Pfu DNA Polymerase as a Hot Start Alternative
... withheld by separation from the reaction by a wax
or the polymerase is reversibly ...
antibody or magnesium wax
beads), AmpliTaq Gold DNA polymerase
antibody or magnesium wax
beads. The 2-kb target, however, could not be
Oil-Free PCR Using the Hot Top Assembly
... loading was quicker and
easier without oil or wax
overlays on reactions.
Stratagenes line of ... cycling and eliminates the need for
oil or wax
overlays on PCR reactions. Because the Hot Top ... cycling and eliminates the need to add oil or wax
reaction tubes. The Hot Top ...
Automated Fluorescent-Tag Cycle Sequencing
... RoboCycler 40 and 96 models, eliminates
the need for an oil or wax
overlay on reactions. The Hot Top Assembly
prevents sample ... RoboCycler 96 temperature cyclers eliminates the
need for oil or wax
overlays on reactions during PCR cycle sequencing, which
makes loading ...
Take off: Canadian biotech industry thrives
... area. This area also includes a large concentration of research hospitals and the seat of the provincial government.
While I could continue to wax
more eloquently on Canadian biotech and particularly the virtues of Toronto biotech, I would never get this column out to you. Needless to say, you ...
Brazilian bio-industry should impress American investors
... include: Chinese, Lithuanians, Greeks, Syrians, Koreans, Polish, Hungarians, and even Jamaicans.
Brazilian biotechnology: Biofuels
I could wax
on even further about this trip, but interestingly during the trip Pope Benedict had just come to Brazil, as had Al Gore. President Bush had been ...
GenomiPhi amplification of FFPE tissue DNA for array CGH
5. Shake gently and spin at 10,500 x g for 15 min (while still hot).
6. Place on ice and remove any hardened wax with pipette tip.
7. Heat sample to 45 °C briefly.
8. Add 100 µl chloroform.
Microfluidic analysis of multiplex
PCR products for the genotyping of
... allelic variants from DNA
extracted from paraffin wax
embedded tissues. The use of template
DNA derived from formalin
fixed and paraffin wax
tissue for the mPCR restricts the
size of the PCR fragments to a
maximum of about 300 bp due to
fragmentation and poor DNA ...
FastStart Taq DNA Polymerase
... better than other hot start systems
Produce results across a wide range of magnesium concentrations
barriers, beads, hot start antibodies, manual hot start,
and the need to set up PCR reactions on ic
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
... RT-PCR kits are actually two-step kits since they require additional
cumbersome manipulations (e.g., buffer additions and the use of wax
RT-PCR Accuracy Is Influenced by DNA Polymerase Fidelity
The frequency of mutations in RT-PCR products is thought to be influenced ...