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Vesicles in Biological Technology

Bristly spheres as capsules

... that close themselves off into capsule-shaped vesicles. The walls of the vesicles consist of a monolayer of the bristly particles. The polymer-coated ... a new, simple method for the controlled production of superstructures. The vesicles could be used to encapsulate drugs, for example, as contrast agents, or as ...

ImmunoVaccine Technologies Broadens Its Patent Protection to Japan

... exclusivity that an effective, long-term immune response to treat a disease can be produced using a vaccine comprising of an antigen, an adjuvant, vesicles known as liposomes, and a hydrophobic carrier. "We have a novel technology that enhances vaccines dramatically, so receiving patent approval is ...

Cellular construction methods emulated

... membranes, surfactants have a hydrophilic segment and a hydrophobic segment and aggregate into structures such as membrane-like double layers or vesicles (bubbles). To imitate the forces involved in protein foldinghydrogen-bridge bonds and hydrophobic interactionsthe team used a disk-shaped gelator, in ...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

... Background Correction system to distinguish pits, vesicles and nuclei from their local ... analysis of Transfluor data, pits and vesicles can be detected and distinguished ... for the expected size of the pits, vesicles or nuclei. The Intensity above local ...

Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000

... β 2-adrenergic receptor internalization into acidic endosomal vesicles uses a red-excited pH-sensitive cyanine dye, CypHer TM 5, which is ... internalization of β 2-adrenergic receptors into acidic endosomal vesicles and would appear to be false positives.

...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

... monitoring of cell surface receptors internalizing into acidic endosomal vesicles upon agonist stimulation (4,5).

Using N-terminal VSV-G tagged ... analysis and screening of receptors that internalize into acidic endosomal vesicles upon ligand binding and activation. Using the IN Cell Analyzer 1000, a ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

... monitoring internalization of cell surface receptors via acidic endosomal vesicles upon agonist stimulation (1, 2).

In the assay, the ... and screening of any receptors that internalize into acidic endosomal vesicles upon ligand binding and activation. Dose response data were obtained for a ...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

... to the extracellular domains of the protein. This interaction leads to downstream events such as mobilization of calcium from intracellular storage vesicles into the cytoplasm of the cell. Improper G q -coupled receptor function is implicated in cancer, neurodegenerative and cardiovascular diseases. ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... of transfected siRNA into discrete foci raised the question of whether the siRNA was being concentrated in lipid vesicles derived from the transfection carrier. To address this question, we repeated our transfections using an NBD-labeled ...
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