Navigation Links
Terminus in Biological Technology

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

... All tubing connected to ports that have their terminus within the vessel below the liquid level (i.e. ... The shake flask is connected to the port terminus using aseptic techniques, and then the clamps are ... disconnected. At this point, the harvest port terminus must be covered up again with sterile cotton and ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... expressed protein. Because EK cleaves at the C terminus of its recognition target, which is also the N terminus of the inserted polypeptide sequence, cleavage ... cleavage of all sequences at the N terminus of the polypeptide of interest and produces ...

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

... an efficient, sitespecific protease that cleaves at the carboxy terminus of its recognition target. Cleavage of fusion proteins that contain the ... to fuse the 5-amino-acid EK target site to the natural amino terminus of the JNK protein. The PCR product was inserted into the ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... activation domain we used for pCMV-AD is derived from the carboxyl terminus of the mouse NF- k B p65 protein. 9 The p65 activation domain has been ... 72 to 390 of murine p53 were fused in frame to the carboxyl (C) terminus of the GAL4-DNA binding domain (pBD-53), and amino acids 84 and ...

RNA Interference and Gene Silencing: History and Overview

... active RISC then targets the homologous transcript by base pairing interactions and cleaves the mRNA ~12 nucleotides from the 3' terminus of the siRNA (3 , 18 , 27 , 29 ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... and tandem consensus sequences for optimal translation initiation in both systems. The c-myc epitope and HIS6 purification tags at the carboxy terminus of the expressed protein allow proteins to be detected and purified, respectively. The tags can be separated from the protein encoded by the cDNA ...
Other Contents
(Date:8/19/2014)... in supermarkets to encourage healthier spending habits? A new ... very question by tracking the purchasing records in a ... rate the nutritional value of foods for sale. , ... Just, PhD, and Brian Wansink PhD, author of Slim ... 150 Hannaford Supermarkets in the Northeastern United States between ...
(Date:8/19/2014)... at retail seafood counters in 10 different states ... consumers to unexpectedly high levels of mercury, a ... a fish of the same species, but from ... offense, according to new research by University of ... seafood is essential to allow consumers to choose ...
(Date:8/19/2014)... seas during warm phases and became extinct during cold ... a link between marine crocodilian diversity and the evolution ... 140 million years. , The research, led by ... and formerly from the University of Bristol, UK is ... crocodiles are ,cold-blooded, animals that mainly live in fresh ...
Breaking Biology News(10 mins):Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3Evolution of marine crocodilians constrained by ocean temperatures 2
(Date:8/20/2014)... WEDNESDAY, Aug. 20, 2014 (HealthDay News) -- Loss of ... help explain why many seniors have trouble falling and ... patients, sleep disruption can be especially severe and often ... researchers. The investigators analyzed data from the Rush ... who enrolled at age 65 and are being followed ...
(Date:8/20/2014)... The Board of Directors of top ... is pleased to announce the expansion of its ... and the surrounding region. In support of this ... AIA has relocated to the firm’s office in Shanghai, ... and design and to better support regional clients. ...
(Date:8/20/2014)... Sockeye salmon that sprint to spawning grounds through fast-moving ... University of British Columbia scientists. , When salmon encounter ... downstream of dams they must move upstream using ... to sprinting for humans. , "Days after sockeye passed ... dying only a short distance from their spawning grounds," ...
(Date:8/20/2014)... Research from Columbia University Medical Center shows that nerves ... and that blocking nerve signals using surgery or Botox ... The study was conducted by the laboratory of Timothy ... PhD, in Norway and is published in today,s issue ... long observed that human and mouse cancers contain a ...
(Date:8/20/2014)... Quincy Bioscience, a Wisconsin-based biotech company, has been ... fastest growing private companies in the nation , for ... Bioscience is honored again to be grouped with the ... Bioscience is manufacturer of Prevagen brand supplements, which contain ... in jellyfish and clinically shown to improve memory. Sales ...
Breaking Medicine News(10 mins):Health News:Seniors' Sleep Woes May Be Linked to Loss of Brain Cells 2Health News:Perkins Eastman Expands Presence in China 2Health News:Perkins Eastman Expands Presence in China 3Health News:Salmon forced to 'sprint' less likely to survive migration 2Health News:Severing nerves may shrink stomach cancers: Botox injections slow growth of tumors in mice 2Health News:Quincy Bioscience Recognized in Inc. 500|5000 List of America’s Fastest Growing Companies for Third Consecutive Year 2
Other TagsOther Tags