Fundamentals of Fermentation: Techniques For Benchtop Fermentors
... All tubing connected to ports that have
within the vessel below the liquid level (i.e. ... The shake flask is connected to the
using aseptic techniques, and then the clamps are ... disconnected. At this
point, the harvest port terminus
must be covered up again with sterile cotton and ...
Expression and Purification of Recombinant Proteins That Have Native Amino
... expressed protein. Because EK cleaves at the C terminus
recognition target, which is also the N terminus
of the inserted polypeptide
sequence, cleavage ... cleavage of all sequences at the N terminus
of the polypeptide of
interest and produces ...
Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
... an efficient, sitespecific
protease that cleaves at the carboxy terminus
of its recognition target.
Cleavage of fusion proteins that contain the ... to fuse the
5-amino-acid EK target site to the natural amino terminus
of the JNK protein.
The PCR product was inserted into the ...
New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
... activation domain we used for pCMV-AD is derived from the
of the mouse NF- k B p65 protein. 9
The p65 activation domain has been ... 72 to 390 of murine p53 were fused in frame to the carboxyl
of the GAL4-DNA binding domain (pBD-53), and amino acids
84 and ...
RNA Interference and Gene Silencing: History and Overview
... active RISC then targets the
homologous transcript by base pairing interactions and cleaves the mRNA
~12 nucleotides from the 3' terminus
of the siRNA (3 ,
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression
tandem consensus sequences for optimal translation initiation in both systems.
The c-myc epitope and HIS6 purification tags at the carboxy terminus of the
expressed protein allow proteins to be detected and purified, respectively. The
tags can be separated from the protein encoded by the cDNA ...