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Subcloning in Biological Technology

Mammalian Expression Vectors with Interchangeable Selectable Markers

... exchange drug-resistance elements without subcloning into such vectors. Cre-Mediated ... conventional restriction digestion and ligation subcloning procedures, site-specific recombination is ... to be used for multiple applications without subcloning the gene of interest. This system features the ...

Electrotransformation of E. coli With M13 DNA

... Introduction M13 vectors have been used extensively for subcloning mainly because the abundant, easily isolated single-stranded ... strain. There is good motivation for using X-Gal and IPTG when subcloning into the M13 mp series vectors because the blue/white color ...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

... and time-consuming, requiring either single-stranded DNA templates 1 , subcloning into specialized vectors 2 , plasmid vectors containing unique ... place in virtually any double-stranded plasmid, eliminating the need for subcloning into M13-based bacteriophage vectors and ssDNA rescue. In addition, the ...

Five Ways to Produce siRNAs

... vectors , which are pre-linearized with the same restriction enzymes and are designed especially for subcloning SECs, are available separately. These ready-to-ligate vectors include a hygromycin, neomycin or puromycin resistance gene to ...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... involved [2] and is therefore usually circumvented by subcloning. Because of the absence of appropriate restriction sites in the DNA fragments, subcloning into another suitable vector and subsequent ligation of the two fragments in this construct was impossible in our case. A standard ligation ...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

... successful amplification of a "difficult" GC-rich template. The QIAGEN PCR Cloningplus Kit enabled quick and efficient subcloning of the amplified insert into the pDrive Cloning Vector. After transformation, a high proportion of transformants ...

A New Lambda Vector for Mammalian Expression

... convenience of plasmid libraries for functional studies. This elegant procedure requires very little hands-on time and eliminates the need for subcloning procedures. High-Level Expression in Mammalian Cells Figure 1 ...
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