Mammalian Expression Vectors with Interchangeable Selectable Markers
... exchange drug-resistance elements without subcloning
Cre-Mediated ... conventional restriction digestion and ligation subcloning
procedures, site-specific recombination is ... to
be used for multiple applications without subcloning
the gene of interest. This
system features the ...
Electrotransformation of E. coli With M13 DNA
M13 vectors have been used extensively for subcloning
mainly because the
abundant, easily isolated single-stranded ... strain.
There is good motivation for using X-Gal and IPTG when subcloning
the M13 mp series vectors because the blue/white color ...
High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
... and time-consuming, requiring either
single-stranded DNA templates 1 , subcloning
into specialized vectors 2 ,
plasmid vectors containing unique ... place in virtually any
double-stranded plasmid, eliminating the need for subcloning
bacteriophage vectors and ssDNA rescue. In addition, the ...
Five Ways to Produce siRNAs
vectors , which are pre-linearized with the same restriction enzymes
and are designed especially for subcloning
SECs, are available separately.
These ready-to-ligate vectors include a hygromycin, neomycin or puromycin
resistance gene to ...
Cloning Based on Efficient Three-Fragment Assembly
... involved  and is therefore
usually circumvented by subcloning. Because of the
absence of appropriate restriction sites in the DNA
into another suitable vector
and subsequent ligation of the two fragments in this
construct was impossible in our case. A standard
Successful PCR amplification and subcloning of a GC-rich DNA fragment
... successful amplification of a "difficult" GC-rich template.
The QIAGEN PCR Cloningplus Kit enabled quick and efficient subcloning
of the amplified insert into the pDrive Cloning Vector.
After transformation, a high proportion of transformants ...
A New Lambda Vector for Mammalian Expression
of plasmid libraries for functional studies. This elegant procedure requires
very little hands-on time and eliminates the need for subcloning
High-Level Expression in Mammalian Cells