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Stringency in Biological Technology

Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms

... parameters that influence the analysis stringency of p-value calculation and call generation. This ... parameter values is only necessary if the stringency of the analysis requires a change from the ... Algorithms incorporate statistically based stringency parameters that control the balance between true ...

Northern Analysis: The Basics

... as indicated. Standard low and high stringency SSC/SDS washes were used to remove ... changes of buffer. Low stringency washes (e.g., with 2X SSC or SSPE) remove the ... solution and unhybridized probe. High stringency washes (e.g., with 0.1X SSC ...

New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays

... – Easily tunable parameters that enable the user to vary the stringency of the analyses. – Elimination of negative expression values ... a1. At concentrations below 8 pM in this experiment, a decrease in stringency (i.e., increase in a1 from 0.04 to 0.1) results in an increasing number of ...

Setting up a kinase profiler with IMAP

... note, lower enzyme concentrations were employed to increase the stringency of the assay and reduce the number of substrate hits. ... assay. Testing with a lower concentration of TIE2 increased the stringency of the SF Tyr assay. The SF Tyr results with a reduced ...

Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B

... of focus as the sample. The most effective way to increase the numerator in the SNR equation is to optimize hybridization and stringency wash conditions to minimize non-specific hybridization. The denominator in the equation is determined by the evenness ...

The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research

... different experiments. Posthybridization treatments After hybridization, stringency washes are carried out at the desired temperature (typically 60C) to remove unbound RNA probes. The robot pipettes the preheated washing ...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

... Polymerase concentration The concentration of Taq polymerase is a critical factor in determining the stringency of a PCR. A high concentration results in a reduction in specificity and incurs unnecessary costs. ...
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