SoluLinK Introduces NanoLink(TM) Streptavidin Magnetic Microspheres
introduction of a new product, NanoLink(TM) streptavidin
Microspheres. These sub-micron particles ... streptavidin
magnetic microsphere. NanoLink(TM) streptavidin
particles are particularly
suited for high ... ) "NanoLink(TM) streptavidin
Magnetic Microspheres represent a
continuation of ...
ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform
... the cost per well to begin at approximately 10 cents, while custom quantitation assays can be run by biotinylating the protein of interest using streptavidin
The new fragment screening assay methodology extends the company's small molecule analysis capabilities - available on ForteBio's ...
Evaluation of FlashPlate in a Helicase Assay
... for a helicase functional assay. Helicase activity was measured on a streptavidin
coated FlashPlate (SMP103), using two DNA oligos. One of the oligos was ... Counter from Packard Instrument Company.
FlashPlate PLUS (SMP103)
#1 (60 ...
Counting FlashPlates on the Wallac MicroBeta
A Reverse Transcriptase Assay was performed using a streptavidin
coated FlashPlate (SMP103). In this assay, a biotinylated DNA ... with upper tube only counting.
[ 3 H]Biotin was titrated on both streptavidin
coated FlashPlates and ScintiPlates, then bound activity was read on the ...
Real-Time Immuno-PCR on the iCycler iQ System, Rev A
... wells were incubated for 30 min at room
temperature with 25 l streptavidin
solution (0.3 g/ml streptavidin
10 mM Tris-HCl, pH 7.5, 50 mM KCl, 2.5 mM MgCl 2 ). ...
ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System
Target Validation and Identification of Novel Disease Indications
... U133 GeneChip array, the Murine Genome U74v2 Set, or the Rat Genome U34 Set. The arrays are hybridized for 24 hours at 45oC, washed and stained with streptavidin
Phycoerythrin (Molecular Probes) in Affymetrix fluidic stations, then detected by fluorometric scanning (Hewlett-Packard GeneArray Scanner). The ...
A DELFIA Assay for Inhibition of PARP
... MgCl 2 ). The reaction was performed at room temperature, for 30 minutes. The plate was then washed 2 times using the DELFIA Platewash. Eu-labelled streptavidin
was diluted in DELFIA Assay Buffer to 200 ng/ well and incubated for 30 minutes at room temperature. The plate was washed 4 times using the DELFIA ...
DELFIA Protein Kinase assays.
Convenient collection of the substrates (peptides, polypeptides or proteins) on coated microtitration plates can be accomplished using streptavidin
plates for biotinylated substrates or glutathione or anti-glutathione-S-transferase plates for substrates expressed with a GST tag.
1. In ...
BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
... peripheral blood mononuclear cells (PBMC). The reagent set isolates Treg cells in two stages. First, negative selection is performed using BD IMag streptavidin
particles with a cocktail of biotinylated monoclonal antibodies that recognizes antigens expressed on erythrocytes, platelets, granulocytes, ...
Tools for Proteomic Science II
... provides a
simple and rapid method for the labeling of proteins with
alkaline phoshatase, biotin, digoxigenin, fluorescein, or
and antibody conjugates recognize
biotin or digoxigenin and fluorescein, and allow the
convenient detection of labeled proteins.
Human Universal cDNA Library Array I
... corresponding mRNAs
used for cDNA synthesis were pooled, biotinylated with photobiotin, and
hybridized in excess with the cDNA. streptavidin
treatment, followed by
phenol extraction, removed cDNA-mRNA hybrids and mRNA so that only the
unhybridized cDNA remained in the ...