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Streptavidin in Biological Technology

SoluLinK Introduces NanoLink(TM) Streptavidin Magnetic Microspheres

... the introduction of a new product, NanoLink(TM) streptavidin Magnetic Microspheres. These sub-micron particles ... streptavidin magnetic microsphere. NanoLink(TM) streptavidin particles are particularly suited for high ... ) "NanoLink(TM) streptavidin Magnetic Microspheres represent a continuation of ...

ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform

... the cost per well to begin at approximately 10 cents, while custom quantitation assays can be run by biotinylating the protein of interest using streptavidin biosensors. The new fragment screening assay methodology extends the company's small molecule analysis capabilities - available on ForteBio's ...

Evaluation of FlashPlate in a Helicase Assay

... for a helicase functional assay. Helicase activity was measured on a streptavidin coated FlashPlate (SMP103), using two DNA oligos. One of the oligos was ... Counter from Packard Instrument Company. Materials streptavidin FlashPlate PLUS (SMP103) Oligonucleotides #1 (60 ...

Counting FlashPlates on the Wallac MicroBeta

... I A Reverse Transcriptase Assay was performed using a streptavidin coated FlashPlate (SMP103). In this assay, a biotinylated DNA ... with upper tube only counting. [ 3 H]Biotin was titrated on both streptavidin coated FlashPlates and ScintiPlates, then bound activity was read on the ...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

... wells were incubated for 30 min at room temperature with 25 l streptavidin solution (0.3 g/ml streptavidin in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 2.5 mM MgCl 2 ). ...

ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications

... U133 GeneChip array, the Murine Genome U74v2 Set, or the Rat Genome U34 Set. The arrays are hybridized for 24 hours at 45oC, washed and stained with streptavidin Phycoerythrin (Molecular Probes) in Affymetrix fluidic stations, then detected by fluorometric scanning (Hewlett-Packard GeneArray Scanner). The ...

A DELFIA Assay for Inhibition of PARP

... MgCl 2 ). The reaction was performed at room temperature, for 30 minutes. The plate was then washed 2 times using the DELFIA Platewash. Eu-labelled streptavidin was diluted in DELFIA Assay Buffer to 200 ng/ well and incubated for 30 minutes at room temperature. The plate was washed 4 times using the DELFIA ...

DELFIA Protein Kinase assays.

... Convenient collection of the substrates (peptides, polypeptides or proteins) on coated microtitration plates can be accomplished using streptavidin plates for biotinylated substrates or glutathione or anti-glutathione-S-transferase plates for substrates expressed with a GST tag. 1. In ...

BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells

... peripheral blood mononuclear cells (PBMC). The reagent set isolates Treg cells in two stages. First, negative selection is performed using BD IMag streptavidin particles with a cocktail of biotinylated monoclonal antibodies that recognizes antigens expressed on erythrocytes, platelets, granulocytes, ...

Tools for Proteomic Science II

... provides a simple and rapid method for the labeling of proteins with alkaline phoshatase, biotin, digoxigenin, fluorescein, or peroxidase. streptavidin and antibody conjugates recognize biotin or digoxigenin and fluorescein, and allow the convenient detection of labeled proteins. ...

Human Universal cDNA Library Array I

... corresponding mRNAs used for cDNA synthesis were pooled, biotinylated with photobiotin, and hybridized in excess with the cDNA. streptavidin treatment, followed by phenol extraction, removed cDNA-mRNA hybrids and mRNA so that only the unhybridized cDNA remained in the ...
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(Date:8/27/2014)... effective doses of the HIV drug maraviroc, a new ... completed before the drug was licensed in 2007, included ... key to removing maraviroc from the body. The current ... protein including nearly half of African-Americans end ... those who lack the protein even when given the ...
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Breaking Biology News(10 mins):Dosage of HIV drug may be ineffective for half of African-Americans 2Researchers change the emotional association of memories 2Researchers change the emotional association of memories 3Researchers change the emotional association of memories 4Stop and listen: Study shows how movement affects hearing 2Stop and listen: Study shows how movement affects hearing 3
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