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Splicing in Biological Technology

Isis and Partners Present New Research at the American Association for Cancer Research 2009 Annual Meeting

... and titled "Molecular Control of RNA Alternative splicing by sigma RNA, a Marker for Carcinomas" showed that antisense inhibition of Malat-1 altered the splicing of multiple mRNAs. Altering splicing results in the production of different variations ...

Seneca Valley High School Student Receives Science Award From MedImmune

... Li for her project entitled "Discovering New splicing Forms in von Willebrand Factor to Understand ... and understanding the application of alternative splicing in individuals. VWD is an inherited bleeding ... was expected to have around 22 to 23 alternative splicing forms. With ...

In vivo visualization of alternative splicing

... developmentally regulated alternative mRNA splicing in a living organism. Dr. Hidehito Kuroyanagi and ... University engineered a transgenic alternative splicing reporter system to monitor the developmentally regulated switching of let-2 alternative splicing in live C. elegans worms. ...

VIRxSYS Publishes New Study on RNA Therapy

... was only expressed in liver cells, and not in other cells, which could have triggered an immune response. The technology used to perform the albumin splicing process is owned by VIRxSYS and is known as spliceosomal mediated RNA trans- splicing, or SMaRT(TM). VIRxSYS now has 39 publications in top tier ...

ExonHit Launches Rat Genome SpliceArray(TM) Products to Expand High Resolution Gene Expression Profiling for use in Disease Modeling and Toxicogenomics

... on the Agilent Technologies and Affymetrix microarray platforms, now expand our product offering and enable researchers to leverage alternative splicing discovery in the rat model system. "ExonHit continues to grow its catalog of arrays for it genomics services. This most recent addition will expand ...

Jivan Awarded US Patent #7,340,349 for Splice Array Analysis

... for DNA microarrays to detect alternative splicing," said Subha Srinivasan, PhD, CEO of JIVAN. "Powerful data analysis is critical to the adoption of splicing arrays, the next generation of expression arrays." The '349 Patent fits in JIVAN's broader strategy of providing a complete solution for splice ...

The GeneZoo or the GeneJungle?

... sequence contiguous to the poly-A tail to perform the clustering, splicing is not a significant factor. We could choose to ignore splice ... is more rigorous, and we rely instead on there being little splicing near the 3 ends. Fig.1 ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... appear to occur in mammalian cells since siRNA made against one splice form of a particular gene does not silence other splicing variants (Tom Tuschl, personal communication, March 2002). Within the cell, siRNA becomes associated with an RNA-induced ...

New Tools for Small RNA Analysis

... play critical roles in a variety of cellular processes, from regulation of mRNA translation, pre-mRNA splicing or transcription to chromosome maintenance and gene imprinting. In addition, two novel classes of small ...

Efficient Cellular Fractionation for RNA and Protein Isolation

... nuclear intronic sequences. Figure 2. Analysis of Fibronectin pre-mRNA Splicing. The mutually exclusive alternative splicing of fibronectin exon EIIIb was analyzed by RT-PCR (35 cycles) using total (T), cytoplasmic (C), and nuclear (N) RNA isolated ...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

... To investigate the bias of these amplification methods, we used yeast as a model eukaryotic organism due to its low level of splicing variants. The experiments included two different cell populations grown in rich and minimal media. ...
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