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Repressor in Biological Technology

JCI table of contents: April 22, 2008

... by a reliance on either cyclin D1 or cyclin D2, such as many individuals with multiple myeloma. TITLE: Identification of kinetin riboside as a repressor of CCND1 and CCND2 with preclinical antimyeloma activity AUTHOR CONTACT: A. Keith Stewart Mayo Clinic, Scottsdale, Arizona, USA. Phone: (480) ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

... 2.2 Transcriptional repressor of known cell-cycle regulatory protein (to be discussed in a ... 13. Onco-1 contained a gene encoding a transcriptional repressor of a known cell cycle regulatory protein. It is not surprising, ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... features designed for inducible high-level protein expression in bacterial cells. The vector contains the hybrid T7/lac O promoter and lac repressor gene (lac I) to regulate protein expression. Therefore, expression is inducible using isopropyl-1-thio-b-D-galatopyranoside (IPTG) in BL21(DE3) ...

Mammalian and Bacterial Expression in One Vector

... cells. Expression of heterologous genes in bacteria is regulated by a hybrid T7/ lacO promoter. The pDual vector was engineered to carry the lac repressor gene (lacI). Expression is therefore inducible using IPTG in bacteria that contain the T7 RNA polymerase. ## In both bacterial and mammalian ...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

... repressed until ligand binding, at which time high-level transcriptional activation occurs (i.e., the heterodimer is converted from a tight repressor to a trans-activator). Using the Complete Control plasmid-based system, induction ratios of greater than 1,000-fold have been achieved in both ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... released by Stratagene. 1 It is derived from the pET-11 vector series and contains the lacI q gene for expression of the Lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 7,8 The vector is ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... released by Stratagene. It is derived from the pET-11 vector series and contains the lacI q gene for expression of the Lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 8 The vector is ...
Other Contents
(Date:4/17/2014)... , Respiratory syncytial virus (RSV) is a ... life-threating illness in very young and elderly populations. ... enters host cells through the fusion protein RSV ... interfering peptides that prevent bundle formation limit RSV ... susceptible to degradation. In this issue of the ...
(Date:4/17/2014)... 16, 2014. Kessler Foundation has been named awardee ... the Department of Defense Spinal Cord Injury Research ... for the randomized, double-blinded, controlled, multi-site clinical trial, ... muscle strength after spinal cord injury. Dr. Forrest ... Research at Kessler Foundation. Two additional sites will ...
(Date:4/17/2014)... cartridge developed at Sandia National Laboratories and recently licensed ... and cheaper. , Bacillus anthracis , the ... all over the world and can cause serious, and ... bacteria can survive in harsh conditions for decades. In ... through skin contact, inhalation of spores or eating contaminated ...
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(Date:4/16/2014)... The Translational Genomics Research Institute (TGen) recently honored two ... their support of TGen,s research into brain, colon and ... Scottsdale. , Catherine Ivy, Founder and President of The ... McCain Leadership Award, named for U.S. Sen. John McCain ...
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