JCI table of contents: April 22, 2008
... by a reliance on either cyclin D1 or cyclin D2, such as many individuals with multiple myeloma.
TITLE: Identification of kinetin riboside as a repressor
of CCND1 and CCND2 with preclinical antimyeloma activity
A. Keith Stewart
Mayo Clinic, Scottsdale, Arizona, USA.
Phone: (480) ...
Functional Cloning Using
ViraPort Retroviral cDNA Expression Libraries
of known cell-cycle regulatory protein
(to be discussed in a ... 13. Onco-1 contained a gene encoding a
of a known cell cycle regulatory protein. It
is not surprising, ...
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression
... features designed for
inducible high-level protein expression in bacterial cells. The vector contains
the hybrid T7/lac O promoter and lac repressor
gene (lac I)
to regulate protein expression. Therefore, expression is inducible using
isopropyl-1-thio-b-D-galatopyranoside (IPTG) in
Mammalian and Bacterial Expression in One Vector
... cells. Expression of heterologous genes in bacteria is
regulated by a hybrid T7/ lacO promoter. The pDual vector was engineered
to carry the lac repressor
gene (lacI). Expression is therefore inducible
using IPTG in bacteria that contain the T7 RNA polymerase. ##
In both bacterial and mammalian ...
pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
... repressed until ligand binding, at which time
high-level transcriptional activation occurs (i.e., the heterodimer is converted
from a tight repressor
to a trans-activator). Using the Complete Control
plasmid-based system, induction ratios of greater than 1,000-fold have been
achieved in both ...
Expression and Purification of Recombinant Proteins That Have Native Amino
by Stratagene. 1 It is derived from the pET-11 vector series
and contains the lacI q gene for expression of the Lac repressor
protein and the hybrid T7 promoter for controlled expression of the inserted
protein coding sequence. 7,8 The vector is ...
High-Level Protein Expression, One-Column Purification, and FLAG
Epitope Tagging in E. coli
... released by
Stratagene. It is derived from the pET-11 vector series and contains the
lacI q gene for expression of the Lac repressor
and the hybrid T7 promoter for controlled expression
of the inserted protein coding sequence. 8 The vector is ...