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Promoter in Biological Technology

New Collaboration Aims To Expand Seed-Based Technologies, Benefit Farmers

... computational approaches, to identify promoter candidates. Once specific promoter sequences are identified, the information can be further used to design ... Padgette, vice president of biotechnology for Monsanto. "A robust promoter toolbox can further leverage Monsanto's extensive gene library and lead to ...

Updated Online Tutorials for DBTSS, Pfam and PDB

... Protein Databank (PDB). DBTSS is a public resource for the analysis of promoter regions. Pfam is a comprehensive database of protein families ... These tutorials will teach users: DBTSS to examine human promoter regions, and those in selected other species as well to locate ...

Debiopharm Group and Moffitt Cancer Center Sign Development and Commercialisation Agreement for Small Molecule Debio 0928 in Early Preclinical Development for Treatment of Solid Tumours

... many types of cancer, could open the door to more effective oncology treatments in the future." "The disruption of the binding of the tumour promoter Raf-1 to the tumour suppressor Rb with Debio 0928 is a novel, first-in-class therapeutic strategy that has the potential for targeting a wide ...

BASF Sues DuPont for Patent Infringement

... patented technology that confers tolerance to a key class of herbicides. Specifically, DuPont's Optimum GAT corn product incorporates BASF's patented promoter which is an important genetic component that enables successful tolerance toward an herbicide class known as AHAS or ALS inhibitors. BASF Plant ...

Tigris Pharmaceuticals Presents Results of Breast Cancer Research at AACR Annual Meeting

... of CYP1A1) from the cytoplasm to the nucleus, and its subsequent interaction with the xenobiotic-response element (XRE) sequence on the CYP1A1 promoter region, causing induction of CYP1A1 transcription. This enzyme expression converts AF to metabolites that are covalently bound to DNA. This results ...

DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors

... ensure optimal repression prior to induction. These vectors are available with either the T7 or T5 promoter. DNA2.0's research has shown that the T5 promoter routinely expresses proteins at higher levels comparable to T7 pET vectors and works in any E. coli host. Cloning into the pJexpress series is free ...

Sigma-Aldrich Introduces MISSION(R) LentiPlex(TM) Pooled shRNA Libraries to Enable Rapid Whole Genome Screening in Primary Cells, Stem Cells or Tumor Cells

... are typically resistant to standard siRNA transfection or other viral delivery systems. In addition, Sigma-Aldrich's shRNA libraries include a U6 promoter for shRNA expression in a wide variety of cell lines including embryonic stem cells. These characteristics mean researchers can now conduct high ...

A New President at the Head of the Genethon Laboratory

... creation, Genethon is today a not-for-profit biotechnology enterprise whose aim is to develop biotherapies for rare genetic diseases. Genethon is the promoter of the first gene therapy trial in humans for gamma-sarcoglycan deficiency. It possesses two clinical batch production units for vectors and is ...

JCI table of contents: Dec. 3, 2007

... to adopt invasive characteristics. These effects of IL-6 were dependent on the protein Notch-3 and led the authors to conclude that, IL-6 is a potent promoter of malignant features in Notch-3expressing normal and tumor stem/progenitor cells of the mammary gland. Similarly, a pro-tumor role for IL-6 in ...

DuPont Joint Venture with Chinese Biotech Firm Strengthens Gene Discovery Research Efforts

... established in 2000 by four prestigious, agribiotechnology-focused academic institutions and has capabilities in functional genomics, trait gene and promoter discovery and molecular breeding technologies for hybrid seeds. BWK is engaged in the business of developing and delivering biotech traits for crop ...

Free shopping in a virtual bazaar of gene regulation data

... accessible manner. In order to demonstrate the advantages and features of PAZAR and its depth of annotation, the researchers used the Pleiades promoter Project collection of brain-linked regulatory sequences as a show case. They have been working internationally with boutique operators and are ...

Ranbaxy to Consolidate Position in Biopharmaceuticals and Speciality Injectables Including Oncology

... by Ranbaxy to other shareholders of Zenotech, at a price of Rs. 160 per share or as determined by SEBI regulations. Post the offer, the existing promoter Group of Zenotech will have a 25% stake in its expanded equity capital. Dr. Jayaram Chigurupati shall continue as the Managing Director. Speaking on ...

Transformation of Nematodes With the Helios Gene Gun

... control of the SV40 early promoter (Figure 3 A and B), or of the C. elegans actin-1 core promoter after Helios gene gun mediated delivery of Au/DNA ... constructs containing the actin-1 core promoter of C. elegans (Files et al., 1983; Krause et al., ...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

... Materials and Methods The promoter to be tested for hormonal or tissue-specific ... 1986; see Koehler et al., 1996, for details of promoter construction). The GUS gene is fused to an enhanced 35S promoter (Kay et al., 1987). An accurate ...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

... are presented in the table. To demonstrate that promoter occupancy was independent of the specific region of the promoter amplified, we examined two different ... sec at 72C, for 30 cycles. Amplified promoter fragments were subcloned into the T/A ...

More siRNA Vectors for RNA Interference

... eliminates any need to include promoter sequence in the siRNA. The ... promoters are very distinct, with the U6 promoter stretching across several hundred base pairs (Kunkel 1989) and the H1 promoter barely exceeding 100 (Myslinski et ...

Enhanced siRNA Delivery and Long-term Gene Silencing

... Choice of promoter Allows Optimal Gene Knockdown Ambion ... these promoters; thus the choice of promoter can affect the level of gene silencing. ... differences were dependent on which promoter was driving siRNA expression. For example, ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... vectors carry an RNA polymerase II-type CMV promoter (human cytomegalovirus ... antibiotic selection, an SV40 promoter expresses one of three ... of mature siRNA or miRNA, the CMV promoter is excellent for stable or transient ...

Drive Long-Term Silencing with CMV Vectors

... resistance gene. The CMV promoter is used to drive siRNA expression. This strong promoter is active in a broad range of cell types and ... siRNA under the control of a polymerase III promoter and an antibiotic resistance ...

Delivering siRNA Using Adenoviral Vectors

... cells Modified CMV promoter efficiently expresses hairpin siRNAs ... mammalian cells and organisms. The modified CMV promoter in the vector efficiently expresses ... noninfected controls. The vector and promoter featured in the p Silencer adeno 1.0-CMV System ...

Five Ways to Produce siRNAs

... RNA polymerase III (pol III) promoter to drive the expression of a small hairpin siRNA ... the most effective combination of promoter and siRNA in a given cell line. In fact, SECs ... (SECs) with a human H1, human U6, or mouse U6 promoter by PCR for use in RNAi ...

GENE SILENCING WITH PCR PRODUCTS

... an RNA Polymerase III (Pol III) promoter adjacent to a hairpin siRNA template ... sequence, or to identify the best combination of promoter and siRNA sequence in the ... The 3' ends of the three different promoter elements provided in the Silencer ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... in mammalian cells. 3 The vector contains the promoter and enhancer region of the human cytomegalovirus ... cells. The vector contains the hybrid T7/lac O promoter and lac repressor gene (lac I) to regulate ... vectors. The vector contains a hybrid bacterial promoter for inducible bacterial expression, the ...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

... element (ISRE) found in the promoter of the 54-kDa interferon-stimulated gene ... repeats of the gamma-activating sequence from the promoter of the guanylate-binding protein. In these plasmids, a basic promoter element (TATA box), and the direct repeats of ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... that uses the cytomegalovirus (CMV) promoter for constitutive expression in a wide variety of ... many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site ... of the prokaryotic b -lactamase promoter to provide kanamycin resistance in ...

Mammalian and Bacterial Expression in One Vector

... prokaryotic systems. The vector contains the promoter and enhancer region of the ... Cells The hybrid T7/lacO phage promoter was tested by carrying out induction ... systems. The vector contains a hybrid bacterial promoter for inducible bacterial expression, the ...

Improved Vectors for PathDetect Trans-Reporting Systems

... plasmids feature the cytomegalovirus (CMV) promoter for expression of fusion proteins in a wide ... luciferase gene controlled by a synthetic promoter that contains the yeast GAL4 ... and offer expression driven by the powerful CMV promoter and the capability for G418 selection of stable ...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

... thus, can be replaced with a cell-type specific promoter of interest. DNA vector-based systems that ... of inducer the heterodimer remains bound at the promoter in a complex with corepressors and ... message transcribed from the CMV promoter ( Figure 1 ). The receptor proteins ...

Yeast Protein Production System Features High Yields and One-Step Purification

... Figure 3 The nmt1 promoter of S. pombe is tightly repressed in the presence ... its removal. 3 When activated, the nmt1 promoter has been shown to be one of the ... S. pombe. 4 For this reason, the nmt1 promoter has been widely used to study gene function ...

Versatile Reporter Vectors for Monitoring Viral Transduction

... protein (hrGFP) downstream of the viral promoter were bright green (emission peak: 506 ... measure of gene expression from the viral promoter in a cell line of choice. In addition, ... gene expression efficiency from the viral promoter in a target cell of choice is ...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

... luciferase gene, which is controlled by a promoter that responds to GAL4 fusions, as does the HLR ... these fusion proteins bind to the promoter and induce luciferase expression. Therefore, ... These fusion trans-activators bind to the promoter in the pFR-Luc reporter plasmid, which contains ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... is located in the MCS downstream of the GAL10 promoter ( Figure 1 ). The gene of interest can ... N-terminal tagging. Comparing GAL1 and GAL10 promoter Strengths We tested the pESC vectors by ... of the GAL1 or GAL10 promoter. To compare the promoter activity under repressed and induced ...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

... range of mammalian cell types. The inducible promoter used in the system is naturally repressed in the ... vector pERV3 facilitates replacement of the CMV promoter with a cell type-specific promoter of interest. ... of the CMV promoter; and the CMV promoter in this construct can be readily replaced with ...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

... in eukaryotic cells. The cytomegalovirus (CMV) promoter allows constitutive expression of the ... of both the prokaryotic b -lactamase promoter to provide kanamycin resistance in bacteria and the SV40 early promoter to provide G418 resistance in ...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

... host for the production of foreign proteins since its alcohol oxidase promoter was isolated and cloned; its transformation was first reported in 1985 ... which catalyzes the first step in the metabolism of methanol. This promoter has been characterized and incorporated into a series of P. pastoris ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... a 420 bp region at the 5' end of the gene, containing the core promoter and the first exon, was amplified using two GC clamped primers ... Results While no new mutations were found in the apoA-I promoter region, we were able to detect, using DGGE, a common ...

Transgenic Zebrafish by Electroporation

... 79 , 6777-6781, 1982). This plasmid uses the Rous sarcoma virus (RSV) promoter to effectively drive expression of chloramphenicol acetyl ... studies since we are interested in tissue- and cell-specific promoter function during development. Hence, our animals, which carry ...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

... genome of PVA has been constructed under the control of a T7 promoter (PVA - T 7 ), f rom which infectious transcripts can be ... under the control of a cauliflower mosaic virus (CMV) 35S promoter (PVA-35S) that can be used directly for inoculation (. Puurand ...

Test More siRNAs for Less

... an 8 base sequence complementary to the 3' end of the T7 promoter primer included in the Silencer siRNA Construction Kit. ... specific oligonucleotide is annealed to the supplied T7 promoter primer. A fill-in reaction with Klenow fragment generates a ...
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