New Collaboration Aims To Expand Seed-Based Technologies, Benefit Farmers
... computational approaches, to identify promoter
candidates. Once specific promoter
sequences are identified, the information
can be further used to design ... Padgette, vice
president of biotechnology for Monsanto.
"A robust promoter
toolbox can further leverage Monsanto's extensive gene
library and lead to ...
Updated Online Tutorials for DBTSS, Pfam and PDB
... Protein Databank (PDB). DBTSS is a public resource for the analysis of promoter
Pfam is a comprehensive database of protein families ... These tutorials will teach users:
to examine human promoter
regions, and those in selected other species as well to locate ...
Debiopharm Group and Moffitt Cancer Center Sign Development and Commercialisation Agreement for Small Molecule Debio 0928 in Early Preclinical Development for Treatment of Solid Tumours
... many types of cancer, could open
the door to more effective oncology treatments in the future."
"The disruption of the binding of the tumour promoter
Raf-1 to the tumour
suppressor Rb with Debio 0928 is a novel, first-in-class therapeutic strategy
that has the potential for targeting a wide ...
BASF Sues DuPont for Patent Infringement
... patented technology that confers tolerance to a key class of herbicides. Specifically, DuPont's Optimum GAT corn product incorporates BASF's patented promoter
which is an important genetic component that enables successful tolerance toward an herbicide class known as AHAS or ALS inhibitors.
BASF Plant ...
Tigris Pharmaceuticals Presents Results of Breast Cancer Research at AACR Annual Meeting
... of CYP1A1) from the cytoplasm to the nucleus, and its subsequent interaction with the xenobiotic-response element (XRE) sequence on the CYP1A1 promoter
region, causing induction of CYP1A1 transcription. This enzyme expression converts AF to metabolites that are covalently bound to DNA. This results ...
DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors
... ensure optimal repression prior to induction. These vectors are available with either the T7 or T5 promoter. DNA2.0's research has shown that the T5 promoter
routinely expresses proteins at higher levels comparable to T7 pET vectors and works in any E. coli host. Cloning into the pJexpress series is free ...
Sigma-Aldrich Introduces MISSION(R) LentiPlex(TM) Pooled shRNA Libraries to Enable Rapid Whole Genome Screening in Primary Cells, Stem Cells or Tumor Cells
... are typically resistant to standard siRNA transfection or other viral delivery systems. In addition, Sigma-Aldrich's shRNA libraries include a U6 promoter
for shRNA expression in a wide variety of cell lines including embryonic stem cells. These characteristics mean researchers can now conduct high ...
A New President at the Head of the Genethon Laboratory
... creation, Genethon is
today a not-for-profit biotechnology enterprise whose aim is to develop
biotherapies for rare genetic diseases. Genethon is the promoter
first gene therapy trial in humans for gamma-sarcoglycan deficiency. It
possesses two clinical batch production units for vectors and is ...
JCI table of contents: Dec. 3, 2007
... to adopt invasive characteristics. These effects of IL-6 were dependent on the protein Notch-3 and led the authors to conclude that, IL-6 is a potent promoter
of malignant features in Notch-3expressing normal and tumor stem/progenitor cells of the mammary gland.
Similarly, a pro-tumor role for IL-6 in ...
DuPont Joint Venture with Chinese Biotech Firm Strengthens Gene Discovery Research Efforts
... established in 2000 by four prestigious,
agribiotechnology-focused academic institutions and has capabilities in
functional genomics, trait gene and promoter
discovery and molecular
breeding technologies for hybrid seeds. BWK is engaged in the business of
developing and delivering biotech traits for crop ...
Free shopping in a virtual bazaar of gene regulation data
... accessible manner.
In order to demonstrate the advantages and features of PAZAR and its depth of annotation, the researchers used the Pleiades promoter
Project collection of brain-linked regulatory sequences as a show case. They have been working internationally with boutique operators and are ...
Ranbaxy to Consolidate Position in Biopharmaceuticals and Speciality Injectables Including Oncology
... by Ranbaxy to other
shareholders of Zenotech, at a price of Rs. 160 per share or as determined
by SEBI regulations. Post the offer, the existing promoter
Group of Zenotech will have a 25%
stake in its expanded equity capital. Dr. Jayaram Chigurupati shall
continue as the Managing Director. Speaking on ...
Transformation of Nematodes With the Helios Gene Gun
control of the SV40 early promoter
(Figure 3 A and B), or of the C. elegans
actin-1 core promoter
after Helios gene gun mediated delivery of Au/DNA ... constructs containing
the actin-1 core promoter
of C. elegans (Files et al., 1983; Krause et al., ...
Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
... Materials and Methods
to be tested for hormonal or tissue-specific ... 1986; see Koehler et al., 1996, for details of promoter
The GUS gene is fused to an enhanced 35S promoter
(Kay et al., 1987).
An accurate ...
Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
... are presented in the table. To demonstrate that promoter
occupancy was independent of the specific region of the promoter
we examined two different ... sec at 72C,
for 30 cycles. Amplified promoter
fragments were subcloned into the T/A
More siRNA Vectors for RNA Interference
... eliminates any need to include
sequence in the siRNA.
The ... promoters are very distinct, with the U6 promoter
several hundred base pairs (Kunkel 1989) and the H1 promoter
exceeding 100 (Myslinski et ...
Enhanced siRNA Delivery and Long-term Gene Silencing
Choice of promoter
Allows Optimal Gene Knockdown
Ambion ... these
promoters; thus the choice of promoter
can affect the level of gene silencing.
... differences were
dependent on which promoter
was driving siRNA expression. For example,
pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
... vectors carry an RNA polymerase II-type CMV promoter
cytomegalovirus ... antibiotic selection, an SV40 promoter
expresses one of three
mature siRNA or miRNA, the CMV promoter
is excellent for stable or transient
Drive Long-Term Silencing with CMV Vectors
... resistance gene.
The CMV promoter
is used to drive siRNA expression. This
is active in a broad range of cell types and
... siRNA under the control of a polymerase III promoter
antibiotic resistance ...
Delivering siRNA Using Adenoviral Vectors
Modified CMV promoter
efficiently expresses hairpin siRNAs
... mammalian cells and organisms. The modified CMV promoter
the vector efficiently expresses ... noninfected controls. The vector
featured in the p Silencer adeno 1.0-CMV System ...
Five Ways to Produce siRNAs
... RNA polymerase III (pol III)
to drive the expression of a small hairpin siRNA ... the most effective combination
and siRNA in a given cell line. In fact, SECs ... (SECs) with a human H1, human U6, or mouse U6 promoter
for use in RNAi ...
GENE SILENCING WITH PCR PRODUCTS
RNA Polymerase III (Pol III) promoter
adjacent to a hairpin siRNA template
... sequence, or to identify the best combination of promoter
siRNA sequence in the ...
The 3' ends of the three different promoter
elements provided in the Silencer ...
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression
... in mammalian cells. 3 The vector
contains the promoter
and enhancer region of the human cytomegalovirus ... cells. The vector contains
the hybrid T7/lac O promoter
and lac repressor gene (lac I)
to regulate ... vectors. The
vector contains a hybrid bacterial promoter
for inducible bacterial expression,
New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction
... element (ISRE) found in the promoter
of the 54-kDa
interferon-stimulated gene ... repeats of the gamma-activating sequence from the promoter
the guanylate-binding protein. In these plasmids, a basic promoter
box), and the direct repeats of ...
Mammalian Expression Vector for Efficient Cloning of PCR Fragments
... that uses the cytomegalovirus (CMV)
for constitutive expression in a wide variety of ... many distinguishing features. The powerful CMV promoter
and the SV40 polyadenylation
site ... of the
prokaryotic b -lactamase promoter
kanamycin resistance in ...
Mammalian and Bacterial Expression in One Vector
systems. The vector contains the promoter
and enhancer region of the ... Cells
The hybrid T7/lacO phage promoter
was tested by carrying out induction
... systems. The vector contains a hybrid bacterial promoter
for inducible bacterial expression, the ...
Improved Vectors for PathDetect Trans-Reporting Systems
feature the cytomegalovirus (CMV) promoter
for expression of fusion proteins in
a wide ... luciferase gene
controlled by a synthetic promoter
that contains the yeast GAL4 ... and offer expression driven by the powerful CMV promoter
the capability for G418 selection of stable ...
pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
can be replaced with a cell-type specific promoter
DNA vector-based systems that ... of inducer the heterodimer
remains bound at the promoter
in a complex with corepressors and ... message transcribed from the
1 ). The receptor proteins ...
Yeast Protein Production System Features High Yields and One-Step
The nmt1 promoter
of S. pombe is tightly repressed in the presence ... its removal. 3 When activated, the nmt1 promoter
has been shown
to be one of the ... S. pombe. 4 For this
reason, the nmt1 promoter
has been widely used to study gene function
Versatile Reporter Vectors for Monitoring Viral Transduction
... protein (hrGFP) downstream of the viral promoter
bright green (emission peak: 506 ... measure of gene expression from the viral promoter
cell line of choice. In addition, ... gene expression efficiency from the viral promoter
in a target cell of
choice is ...
Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion
... luciferase gene, which is controlled
by a promoter
that responds to GAL4 fusions, as does the HLR ... these fusion proteins bind to the promoter
luciferase expression. Therefore, ... These fusion trans-activators
bind to the promoter
in the pFR-Luc reporter plasmid, which contains ...
Epitope-Tagging Vectors for Functional Analysis in Yeast
... is located in the MCS downstream of the GAL10 promoter
1 ). The gene of interest can ... N-terminal tagging.
Comparing GAL1 and GAL10 promoter
We tested the pESC vectors by ... of the GAL1 or GAL10 promoter. To compare the promoter
under repressed and induced ...
Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in
... range of mammalian cell types. The
used in the system is naturally repressed in the ... vector pERV3
facilitates replacement of the CMV promoter
with a cell type-specific promoter
of interest. ... of the CMV promoter; and
the CMV promoter
in this construct can be readily replaced with ...
An Epitope Tagging Vector for Gene Expression in Mammalian Cells
... in eukaryotic cells. The cytomegalovirus (CMV) promoter
allows constitutive expression of the ... of
both the prokaryotic b -lactamase promoter
provide kanamycin resistance in bacteria and the SV40 early promoter
provide G418 resistance in ...
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
... host for the production of foreign proteins since its alcohol
was isolated and cloned; its transformation was first reported in 1985 ... which catalyzes the first step in the
metabolism of methanol. This promoter
has been characterized and incorporated into a series of
P. pastoris ...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... a 420 bp region at the 5' end of the gene, containing
the core promoter
and the first exon, was amplified using two GC clamped
primers ... Results
While no new mutations were found in the apoA-I promoter
region, we were
able to detect, using DGGE, a common ...
Transgenic Zebrafish by Electroporation
... 79 , 6777-6781, 1982). This plasmid uses the Rous sarcoma virus (RSV) promoter
to effectively drive expression of chloramphenicol acetyl ... studies
since we are interested in tissue- and cell-specific promoter
during development. Hence, our animals, which carry ...
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
of PVA has been constructed under the control of a T7 promoter
T 7 ), f rom which infectious transcripts can be ...
under the control of a cauliflower mosaic virus (CMV) 35S promoter
that can be used directly for inoculation (. Puurand ...
Test More siRNAs for Less
... an 8 base sequence
complementary to the 3' end of the T7 promoter
primer included in
the Silencer siRNA Construction Kit. ... specific
oligonucleotide is annealed to the supplied T7 promoter
fill-in reaction with Klenow fragment generates a ...