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Primer in Biological Technology

Jivan Focuses on Solutions for Transcriptome Sequencing

... for targeted sequencing with mRNA capture arrays, mRNA specific PCR primer designs for targeted amplification, and software for analyzing mRNA ... genes or mRNAs with low expression, Jivan will offer mRNA Prime(TM) PCR primer designs specific to each observed mRNA. To analyze RNA sequencing ...

New Report Just Published: World Prostate Cancer Therapeutics Market Report

... 2004 (includes corresponding Graph/Chart) II-18 Prostate - Structure II-18 Pituitary Gland II-19 Testes II-19 Prostate Cancer - A primer II-20 Key Facts to Gaze at II-20 Table 13: Lifetime Probability of Developing Cancer (in Men) by Type in the US, 2002-2004 II-20 ...

Event Alert: 'SPL R4 Compliance and its Impact on Regulatory Submission Processes'

... who are modifying in-house systems or evaluating new technologies to meet the new SPL R4 requirements. The webinar is designed as a "how to" primer on complying with SPL R4. Attendees will learn about: Best practices for complying with SPL R4 Accelerating the ...

Waters and University of Warwick Sign Research Agreement Designed to Foster Adoption of Novel MS Technologies

... for life scientists adopting cutting edge MS-based research strategies." For more information on MS technologies, Waters recently published a MS primer complete with descriptions on modern MS practices, who uses mass spectrometers, the various types of MS, the importance of mass accuracy and ...

Volatile Economy / Fundraising Challenges Encourage Business Combinations Among Biotech Companies According to New Survey from CPA Firm Rothstein Kass

... the topic, and a highly-sought counselor to families with significant global resources, and their advisors. He is co-author of Fortune's Fortress: A primer on Wealth Preservation for Hedge Fund Professionals . www.RussAlanPrince.com Hannah Shaw Grove is a widely recognized author, columnist, ...

RainDance Technologies Delivers RDT 1000, Sequence Enrichment Solution to JCVI

... RainDance delivers its RDT 1000 and expert training to selected premier organizations in genomic research. RainDance will also provide customized PCR primer libraries designed to selectively amplify loci of interest for research initiatives at JCVI. "The program provides an opportunity for some of the ...

Gerson Lehrman Group and BIO Collaborate to Identify Scientific Expertise for the Seventh Annual BIO Investor Forum

... -- Epilepsy -- Fibromyalgia -- Lung Cancer -- Neurodegenerative Diseases -- Novel Solid Tumor Targets -- Osteoporosis -- Pre-ASH primer "With the financial markets experiencing an unprecedented level of volatility, investment professionals place a greater premium than ever before on ...

Norgen Biotek Corp. Launches Four New Kits for the Purification of RNA and DNA from Plants and Fungi

... and can be used in a number of downstream applications including Real-Time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. With the Plant/Fungi DNA Isolation Kit total DNA is isolated including genomic DNA, mitochondrial DNA and ...

Newsweek International Editions: Highlights And Exclusives, May 19, 2008

... To Stop The Food Riots. (Atlantic and Latin America editions). Senior Editor Rana Foroohar opens this special report on the world food crisis with a primer on the incredibly political nature of food. As food prices spiral out of control, the worry is that millions more of the world's poorest will also be ...

febit to Introduce Geniom RT Analyzer(R) to U.S. Market at RNAi World Congress

... temperature control optimizes hybridizations and enables the use of enzymatic reactions directly on the microfluidic array for signal enhancement and primer extension assays. "We have brought together in one benchtop tool the automation of multiple processes involved with the identification and ...

Gentelligent, Inc. Asks 'Is your DNA in Excel?'

... it's kind of surprising that we're the first company to bring these functions to Excel." BasePro allows users to do everything from determining primer melting temperatures and molecular weights to automatically expanding and contracting IUPAC codes and identifying sequence repeats. According ...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

... step may be necessary to identify those primer combinations that will generate sufficient unique ... allows researchers to choose from 128 possible primer combinations that have been tested across several ... DNA Analyzers, in conjunction with optimized AFLP primer kits and GeneMapper Software v3.7 or higher, ...

Simple Approaches for Optimization of RT-PCR

... is also paid to fundamental issues such as primer design (6,7) , RNA isolation (8,9) , and use of ... (total RNA: 1 pg to 1 μg) Forward primer (10μM) 2 μl (0.4μM in 50 μl reaction) Reverse primer (10μM) 2 μl (0.4μM in 50 μl ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... of L. monocytogenes, but all utilize a primer set that is specific for this species. In such ... In such cases one can use a primer set that anneals to a sequence of the ... amplified by PCR using a general primer set and the Gene CyclerTM. The approach permitted ...

Comparison of Commercial Dye Terminator Removal Kits, Rev A

... reactions. Too much or too little template or primer may result in reads that are difficult ... acquired from Promega. The primer used was a custom-ordered T7 primer (Operon). Half-reaction volumes (half ...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

... on the same oligonucleotide primer. The UniPrimer primer has a universal format, as it will ... (2.5 mM) 90 l lectin gene forward primer (2.5 M) 90 l lectin gene reverse primer (including recognition sequence) (0.5 ...

Real-Time PCR: General Considerations, Rev A

... are general laboratory practices, template and primer design, and reaction components and ... you should design and optimize the primer sets prior to developing the probe. This ... will guide you in the development of an optimized primer set for a quantitative real-time ....

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... the use of a Cy5-labeled reverse primer that is incorporated into the amplified PCR ... Following amplification with the Cy5-labeled primer in the presence of a hybridization ... Methods primer and Probe Design The forward (sense) ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

... in the commercial protocol. primer and Molecular Beacon Design The PCR ... The melting temperatures (T m ) of the primer and probe sequences were determined by Oligo ... specificity of PCR, several different primer pairs were initially designed for GAD67 mRNA ...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

... (C T > 34) of the 128 bp primer set represent primer-dimers and not specific ... vs. Mix A In order to test whether primer design can affect the reproducibility ... Duplicate reactions using each primer pair with each reaction mix (A or iQ supermix) ...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

... as nonspecific products and inefficient primer association with target, which dramatically affect the quality of the data. Proper primer design ensures that the chosen primers ... (Life Technologies) 5 l 100 M forward primer (see Table) 5 l 100 M ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... using a GC-clamped version of the universal primer 16SAR 3 and an oyster-specific primer 16SOB. 4 Although most authors recommend ... 200 M of each dNTP, 10 pmol of each primer and 13 l of template DNA (concentration ...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

... 0.2 l 100 M α-tubulin forward primer (5'-CCAAGCTGGAGTTCTCTA-3', Operon) ... 0.2 l 100 M α-tubulin reverse primer (5'-CAGAGTGCTCCAGG-3', Operon) ... 0.15 l 100 M GAPDH forward primer (5'-CATGTTCCAATATGATTCCAC-3', Operon) ...

Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

... amplification. 8 Nested primer sets were used for the two rounds of PCR. For the ... substances in the soil DNA samples. The following primer set was used to amplify a 200bp ... in a large number of samples. primer sets for other specific functions or ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... to Borresen et al., using a 20-mer primer (5-ATCCTGAGTAGTGGTAATCT- 3) and a 60-mer primer ... dNTP, Taq DNA polymerase (Perkin-Elmer), 20-mer primer (25 pmol) and ...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

... DGGE patterns, combinations of these primer sets were evaluated (Michiels et al. ... MgCl 2 , 200 nM each dNTP, 800 nM each primer (MP 1: exon 1 + exon 4 + exon 13, MP2: ... amplified fragments with the non-GC clamped primer of each exon, using the Applied ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... of the single base change within the primer resulted in the site-directed mutagenesis of the ... completed using the cloned material. primer DESIGN The design of DGGE primers follows the general principles of PCR primer design with respect to sequence ...

Primer Walking

... Sequencing Agencourt offers primer walking sequencing of PCR products, plasmid ... of the service include: Proprietary primer design software High quality ... Service Offering Our comprehensive primer walking and assembly service includes the ...

Ready for Real-time in 5 Minutes

... Applied Biosystems pre-designed primer and probe sets for real-time PCR are ... using a TaqMan Gene Expression Assay primer and probe set specific for GSK-3. ... using a TaqMan Gene Expression Assay primer and probe set specific for VEG-F. ...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

... can be used directly for RT-PCR, as long as primer pairs span exon-exon boundaries. Minus ... cell lysates for qRT-PCR using TaqMan primer & Probe Sets. Relative to the negative ... Knockdown of Gene Expression with TaqMan primer and Probe Sets. HeLa cells were ...

Designing a Successful qRT-PCR Experiment

... Probes: We recommend using primer design software for both ... that will choose probe and primer sequences simultaneously such as primer Express (ABI)). For eukaryotic targets, use ...

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

... of the desired DNA without generating unnecessary primer assembly products. DNA ... shown above), while competitor kits resulted in primer assembly products with 0ng of input ... PCR (45 cycles) using 12 different primer sets. This data shows the targeted genes were ...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

... Agilent 2100 bioanalyzer as described below. primer pairs used for amplification in the PTC-100 ... cycler consisted of one fluorescently labeled primer and one unlabeled primer for each gene. These NT and CT PCR ...

Thermal Cycling Profile for Standard PCR

... to snap-back very quickly, preventing efficient primer annealing and extension, or leading to ... primer annealing For most purposes, ... optimized empirically. The choice of the primer annealing temperature is probably the most ...

PCR reaction components

... genomic DNA, the Human tPA Control primer Set from Roche Applied Science (Cat. No. ... assay success. For convenience, several primer design software programs are available. These can be used to ensure that the primer sequences have the following general ...

Trouble-shooting: No product

... primer annealing temperature is too high ... primer annealing temperature is too high or too low primer annealing temperature is typically 50-60C (may ...

Trouble-shooting: Non-specific bands

... primer annealing temperature is too low primer annealing temperature is typically 50-60C (may be higher or lower based on primer sequence and buffer components). ...

Trouble-shooting: Low yield

... primer annealing temperature is too high primer annealing temperature is typically 50-60C (may be higher or lower based on primer sequence and buffer components). ...

C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

... temperature reduces nonspecific primer binding. Ensures high fidelity first ... because the enzyme can use either oligo(dT) primer or anchor primer at temperatures up to 60C. ...
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