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Polyadenylation in Biological Technology

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... efficiency and 1-hour ligation. However, the pCMV-Script vector has many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is ...

Mammalian and Bacterial Expression in One Vector

... vectors. Yet mammalian and bacterial plasmid vectors vary considerably in their choice of promoters, the presence of splice signals and polyadenylation sites, as well as their respective translation initiation sequences. In fact, the differences between bacterial and mammalian expression vectors ...

High-Titer Retroviral Vectors for Gene Delivery

... region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse ...

A New Lambda Vector for Mammalian Expression

... 6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... with a shuttle vector. Fig.2 The pShuttle-CMV vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal. The pShuttle vector contains only a multiple cloning site in which an entire expression cassette can be inserted. The regions indicated ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) signal provides the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the bait vector, target (prey) ...
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