Navigation Links
Polyadenylation in Biological Technology

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... efficiency and 1-hour ligation. However, the pCMV-Script vector has many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is ...

Mammalian and Bacterial Expression in One Vector

... vectors. Yet mammalian and bacterial plasmid vectors vary considerably in their choice of promoters, the presence of splice signals and polyadenylation sites, as well as their respective translation initiation sequences. In fact, the differences between bacterial and mammalian expression vectors ...

High-Titer Retroviral Vectors for Gene Delivery

... region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse ...

A New Lambda Vector for Mammalian Expression

... 6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... with a shuttle vector. Fig.2 The pShuttle-CMV vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal. The pShuttle vector contains only a multiple cloning site in which an entire expression cassette can be inserted. The regions indicated ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) signal provides the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the bait vector, target (prey) ...
Other Contents
(Date:8/20/2014)... 20, 2014) Women who are poor experience higher ... with elevated levels of the stress hormone, putting ... life, according to a new research from the University ... in the American Journal of Human Biology , ... relate it directly to the socioeconomic status of their ...
(Date:8/20/2014)... new gene therapy developed by researchers at the University ... protect mice from a life-threatening heart condition caused by ... avenue," said Yi Lai, Ph.D., the leading author of ... School of Medicine,s Department of Molecular Microbiology and Immunology. ... this could lead to a treatment for people with ...
(Date:8/20/2014)... about 4,000 members, it triggers an important first stage ... type of comb used for rearing male reproductive, called ... Neurobiology and Behaviour at Cornell University, led by Michael ... colonies. The results are published in Springer,s journal ... Reproduction isn,t always a honeybee colony,s top priority. ...
Breaking Biology News(10 mins):CU Denver researcher shows economic disparities impact infant health 2Gene therapy protects mice from lethal heart condition, MU researchers find 2Worker bees 'know' when to invest in their reproductive future 2
(Date:8/20/2014)... Botanica Day Spa ... the new and popular stem cell line from ... and wrinkles and naturally repairs the skin. The ... new service by offering clients a complimentary dermaplaning ... facial. , Pevonia® continues to be a global ...
(Date:8/20/2014)... of Manchester and Auckland suggest that both major forms of ... The findings, published today in the FASEB Journal ... and type-2 diabetes are both caused by the formation of ... The results, based on 20 years, work in New Zealand, ... down and potentially reversed by medicines that stop amylin forming ...
(Date:8/20/2014)... The Workgroup for Electronic Data ... the use of health IT to create efficiencies ... for its annual fall conference, WEDI-Con 2014: The ... will take place at the HYATT Regency in ... diverse cross-section of payers, providers, government regulators, industry ...
(Date:8/20/2014)... 20, 2014 The National Association ... $1.24 million Science Education Partnership Award (SEPA) grant ... NAHN’s collaborative project with the Hispanic Communications Network ... , This collaborative NIH R25 program has been ... linguistic diversity among health professionals by recruiting and ...
(Date:8/20/2014)... 20, 2014 Attendees of Seedstock’s 3rd ... peak at Los Angeles’ first multi-faceted food production business ... a blossoming 1.5-acre high school campus urban farming operation ... farm in the L.A. Art District. , The field ... urban agriculture, will kick off Seedstock’s “Reintegrating Ag: Local ...
Breaking Medicine News(10 mins):Health News:New Stem Cell Facial Unveiled at Botanica Day Spa 2Health News:New Stem Cell Facial Unveiled at Botanica Day Spa 3Health News:Scientists show type-1 and type-2 diabetes are caused by same underlying mechanism 2Health News:WEDI Releases Full Conference Agenda for Its Annual Fall Conference – WEDI-Con 2014 2Health News:WEDI Releases Full Conference Agenda for Its Annual Fall Conference – WEDI-Con 2014 3Health News:National Association of Hispanic Nurses (NAHN) Receives Five-Year Federal Grant from the National Institutes of Health (NIH) 2Health News:National Association of Hispanic Nurses (NAHN) Receives Five-Year Federal Grant from the National Institutes of Health (NIH) 3Health News:Seedstock Sustainable Agriculture Conference’s Urban Farm Field Trip to Offer First Look at Los Angeles’ Premier Food Business Incubator 2Health News:Seedstock Sustainable Agriculture Conference’s Urban Farm Field Trip to Offer First Look at Los Angeles’ Premier Food Business Incubator 3
Other TagsOther Tags