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Polyacrylamide in Biological Technology

Caliper Life Sciences Introduces Staccato Protein Workstation for Automated Protein Sample Preparation & Analysis

... LabChip GXII is Caliper's high throughput system for analyzing protein size, concentration, and purity and serves as a replacement for traditional polyacrylamide gel electrophoresis (PAGE). Since its launch in July, 2008, the LabChip GXII platform has been widely adopted by high throughput protein analysis ...

LI-COR Introduces the Odyssey(R) Two-Color NIR Fluorescent Protein Molecular Weight Marker

... Molecular Weight Marker is pre-stained and fluoresces in the 700 and 800 nm channels of the Odyssey System. The marker was designed for use with polyacrylamide gels and can be used to visualize the progress of protein separation during electrophoresis, to monitor transfer efficiency to Western blot ...

Using catalysts to stamp nanopatterns without ink

... Toones team chose as a catalyst the biological enzyme exonuclease I, derived from the bacterium E. coli. In one set of experiments, the polyacrylamide stamp pattern bearing the tethered enzymes was then pressed on a surface of gold that had been covered with a uniform coating of single-stranded DNA ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... migrates more slowly through a polyacrylamide gel than does an undegraded, helical, ... strength of sample, etc.) than other polyacrylamide gel electrophoresis procedures. 7 To further ... The gel also contained a gradient of polyacrylamide ranging from 10 to 20%, made from a ...

Heteroduplex Analysis Using the DCode System

... conformation can be detected on polyacrylamide gels due to slower migration than the ... in a gel from homoduplex molecules. polyacrylamide analogs have been developed, such as ... samples when compared to conventional polyacrylamide gels. 2 In this experiment, we show ...

The Bio-Rad Silver Stain, Rev E

... as a general protein stain useful in polyacrylamide gel analysis in 1979 by Merril et al. 54 This ... of subnanogram amounts of RNA in polyacrylamide gels in the presence and absence of protein by ... Moreno MR et al., Silver staining of proteins in polyacrylamide gels: increased sensitivity through a ...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

... cellular macromolecular components. 24 Native polyacrylamide gel electrophoresis (PAGE) of the ... Figure 1. NATIVE PREPARATIVE polyacrylamide GEL ELECTROPHORESIS AND ELECTRO-ELUTION Four percent native polyacrylamide gels, 1.5 mm thick, and containing a ....

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... above. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) low molecular ... of the -lactoglobulin A, B, and C Variants Using polyacrylamide Gel Electrophoresis, ... transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... developed by using continuous-elution polyacrylamide gel electrophoresis (CE-PAGE). This ... was used to perform continuous-elution polyacrylamide gel electrophoresis (CE-PAGE) with ... cells) was layered onto a 4% (1.5 cm) polyacrylamide stacking gel with a 10% (8 ...

Acrylamide Polymerization A Practical Approach

... resolution and flexibility possible with polyacrylamide gel electrophoresis (PAGE) has led to ... takes a practical approach to the preparation of polyacrylamide gels. Its purpose is to provide the ... Mechanism of Polymerization polyacrylamide gels are formed by copolymerization of acrylamide ...

Detecting miRNA & siRNA

... with gel loading buffer, analyzed on a denaturing polyacrylamide gel, and exposed to film. Figure 1A ... nt) were analyzed on a 15% denaturing polyacrylamide gel. (B) The same experiment as in ... exposure). RNAs were analyzed on 15% denaturing polyacrylamide gels. As a loading control, the same ...

HotStarTaq PCR Handbook

... the primers on a denaturing polyacrylamide gel. ... the primers on a denaturing polyacrylamide gel. ... the primers on a denaturing polyacrylamide gel. ...

QIAGEN Multiplex PCR Handbook

... for choosing the optimal percentage of agarose), polyacrylamide gel electrophoresis, or capillary ... the optimal percentage of agarose), polyacrylamide gel electrophoresis, or capillary ... of the primers on a denaturing polyacrylamide gel. If necessary, make new dilutions ...

Four New CastAway Precast Gels

... CastAway system, applications requiring native polyacrylamide gels, such as SSCP, can now be run in a ... CastAway gels are composed of 6% polyacrylamide in 1X TBE. Native polyacrylamide gels can be advantageous in applications such ...

Map and Link Human Genetic Disorders with SSLP Analysis

... just a few bases. With the CastAway denaturing polyacrylamide gels,* it is now easy to separate the PCR ... In a novel approach, we used CastAway precast polyacrylamide gels instead of pouring our own gels. The ... products were analyzed on a denaturing polyacrylamide gel to resolve allelic differences that ...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

... volume of 2.5 ml (1.5 mg protein/ml) was applied to a 7.5% polyacrylamide separating gel poured to 5.5 cm height and a 1 cm high stacking ... 0.001% bromophenol blue). After being electrophoresed on 7.5% polyacrylamide gels, separated proteins were electrophoretically blotted to ...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

... volume of 2.5 ml (1.5 mg protein/ml) was applied to a 7.5% polyacrylamide separating gel poured to 5.5 cm height and a 1 cm high stacking ... 0.001% bromophenol blue). After being electrophoresed on 7.5% polyacrylamide gels, separated proteins were electrophoretically blotted to ...

Rapidly Prepare Probes and Markers for Small RNA Analysis

... probe, and purified IVT probe were resolved on a 15% denaturing polyacrylamide gel. Figure 2. Sensitive ... miRNA Detection Kit. Samples were analyzed on a 15% denaturing polyacrylamide gel. Autoradiography was for 16 hours at 80C. ...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... RNA (1 g) was fractionated using a 15% denaturing polyacrylamide gel. The RNA was transferred to a positively charged ... radiolabeled products were fractionated by denaturing polyacrylamide gel electrophoresis and quantitated using the Storm ...

Isolate Total RNA and Protein From the Same Sample

... (left top). Ten g of total protein was resolved on a 10% SDS polyacrylamide gel and analyzed by Western blot with an anti-GAPDH antibody ... agarose gel stained with EtBr or resolved on a 15% denaturing polyacrylamide gel and analyzed by Northern blot with a 5S rRNA probe 5' ...

miRNA Expression in White Blood Cells

... Kit. Purified RNA (1 g) was analyzed on a 15% denaturing polyacrylamide gel stained with ethidium bromide (left panel). let-7 miRNA, ... and analyzed by ethidium bromide staining on a 15% denaturing polyacrylamide gel. RNA yields ranged from about 20-40 g per 10 ml blood ...

New Tools for Small RNA Analysis

... RNase inactivation/precipitation procedure, run on a denaturing polyacrylamide gel, and visualized by autoradiography. In this assay, ... Gel Purification. miRNAs were gel purified on a 15% denaturing polyacrylamide gel from 150 g of mouse lung or thymus total RNA using standard ...

Identification of Differentially Expressed Gene Products with the CastAway System*

... in RAP-PCR and DD are subjected to gel analysis using high-resolution polyacrylamide gel electrophoresis (PAGE). Bands of interest are excised from the gel, ... was resolved for 2 hours at 50 watts on a CastAway prepoured 6% polyacrylamide gel. The gel was fixed and dried according to the protocol ...

Fractionating DNA Fragments Generated by Differential Display PCR

... order to successfully apply DDPCR, high-quality, reproducible denaturing polyacrylamide gels are required to fractionate PCR fragments. Stratagenes CastAway ... are provided polymerized, the time required to prepare the denaturing polyacrylamide gel for fractionating the DNA fingerprint is dramatically reduced. ...

Automated Fluorescent-Tag Cycle Sequencing

... cycle sequencing products are loaded onto a vertical denaturing polyacrylamide gel and separated by gel electrophoresis. Automated gel reading systems ... of both sets of reactions were loaded onto a 4.8% Gene Page Plus urea polyacrylamide gel and run on an ABI PRISM 377 DNA sequencer. Results ...

Evaluation of FlashPlate in a Helicase Assay

... replication and playing an important role in DNA repair. Conventional helicase functional assays require that unwound DNA products be analyzed on polyacrylamide gels, which have major constraints in high throughput screening (HTS) applications. Preparing the gels is a time-, labor-, and material-intensive ...

ECL Semi-dry Blotters

... with and without built-in power supplies to give maximum flexibility • Complete, uniform electrotransfer of proteins from polyacrylamide gels in less than 1 h • Minimal buffer use—just enough to saturate blotting papers and membranes • ...

Protein Truncation Tests Using the DCode System

... were performed in 12.5 ml with 6 Ci of 35 S-methionine. Protein products were run on a 16 x 20 cm, 14% SDS-PAGE denaturing polyacrylamide gel using the DCode universal mutation detection system. The samples were run at room temperature in 1x Tris/Glycine/SDS buffer ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

... 2,3 Approximately 200 ng of PCR product were mixed with 2x bromophenol blue loading dye and electrophoresed in a 6% polyacrylamide gel (acrylamide/bis 37.5:1) containing 7 M urea, 20% formamide, 2% glycerol in 1.25x TAE buffer (50 mM Tris-Cl, 25 mM acetic ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... gel electrophoresis was carried out using the DCode system (Bio-Rad). Fifteen l of PCR product was loaded on a gel containing a polyacrylamide gradient ranging from 612% and a gradient of urea and formamide. The gels were run in 1x TAE buffer, at 80 V overnight. After ...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

... 1991), but antigenicity appears to be strain dependent. A study is currently underway in our laboratory using twodimensional polyacrylamide gel electrophoresis (2-D PAGE) to identify antigens and virulence factors of this bacterium that may be targeted for the development ...

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

... membranes without glass fiber filters, J Prot Chem 9, 254255 (1990) Towbin H et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc Natl Acad Sci USA 76, 43504354 (1979) Yuen SW et ...

Imaging and Analysis of DIGE Product Information Sheet, Rev B

... obtained with the other two dyes. Equal amounts of the three labeled protein mixtures are combined and run on the same 2-D polyacrylamide gel, eliminating any gel-to-gel variation. Imaging Image Acquisition With the Molecular Imager FX ...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

... and the specific product, or the primer-dimer only. The melt-curve data were confirmed by resolving the amplified products on a polyacrylamide gel (Figure 3). This analysis demonstrates that primer-dimers are present in all of the amplification reactions. Since SYBR ...

Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C

... be identified and how to do it, Biotechnol Genet Eng Rev 13, 1950 (1996) Wilm M et al., Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry, Nature 379, 466469 (1996) Yates JR, Protein structure analysis by mass ...

Enzyme Purification With the Econo-Pac Q Cartridge

... using snake venom as a model. Students use various separation techniques, such as ion exchange and gel filtration chromatography, and polyacrylamide gel electrophoresis. Shown below are typical data from university classroom experiments. Two ion exchange columns are ...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

... and storage at 20C. The purity of the protein was determined by Tris-Tricine SDS-PAGE (Schagger and von Jagow 1987) in a 10% polyacrylamide resolving, 3% stacking gel using the Mini-PROTEAN II slab cell (Figure 2). Gels were stained using the Bio-Rad Silver Stain ...

Oligonucleotide Purity Analysis by Capillary Electrophoresis

... The dissolved polymers act as the sieving matrix and provide separations of oligonucleotides substantially equivalent to polyacrylamide and agarose gel electrophoresis. 1, 2 This method is called dynamic sieving capillary electrophoresis (DSCE). ...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

... the formation of spurious by-products during the amplification process. 4, 5 DGGE was performed with the DCode system. The polyacrylamide gels (7.5%) contained a denaturing gradient from 40 to 70% (100% denaturant: 7 M urea and 40% formamide) and were run in 0.5x ...
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