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Phosphatase in Biological Technology

Sigma-Aldrich Introduces Precisio(TM) Kinases for High-Throughput Screening and Kinase Profiling

... "Combined with Sigma's existing product lineup for kinase and phosphatase research, this new offering facilitates a greater understanding of ... complement Sigma-Aldrich's existing offerings in the field of kinase and phosphatase research, including kinase antibodies, substrates, inhibitors, activators ...

Scientists identify gene vital to early embryonic cells forming a normal heart and skull

... of the study. "This leads to anatomical and functional deficits so severe that it precludes viability of the developing fetus." SHP2 is a tyrosine phosphatase an enzyme that helps trigger a cascade of biochemical reactions in cells as they specify to form certain tissues. Although the study was ...

Enobia Completes Target Enrollment in Clinical Trial for Infantile Hypophosphatasia

... that in some cases stops ambulation. About ENB-0040 ENB-0040 is a subcutaneous enzyme replacement therapy of tissue non-specific alkaline phosphatase (TNSALP) fused to a patented bone targeting peptide. Preclinical studies in the "knockout" mouse model of severe hypophosphatasia showed that ...

Enobia Pharma Presents PreClinical Data Showing ENB-0040 Significantly Improved Survival and Healed Skeletal Manifestations of Severe Hypophosphatasia in Mice

... some cases stops ambulation. About ENB-0040 ENB-0040 is a fusion protein that includes the catalytic domain of human tissue non-specific alkaline phosphatase (TNSALP), an immunoglobulin Fc domain and a patented anionic peptide used to target the enzyme to bone. Preclinical studies of ENB-0040 in the ...

New 200 mg Vial for Eloxatin(R) (Oxaliplatin Injection) Offers Greater Convenience and Efficiency of Preparation

... residual paresthesia at 18-month follow-up -- Hepatotoxicity, as evidenced in the adjuvant study by increase in transaminases and alkaline phosphatase was observed more commonly in the Eloxatin(R) combination arm. The incidence of increased bilirubin was similar on both arms. Changes noted ...

Highly Active and Removable AffinityTagged Lambda Phosphatase

... Dualspecificity Lambda phosphatase useful for dephosphorylation serine, threonine ... Systems, Inc. Stratagene's recombinant Lambda phosphatase is a dualspecificity protein phosphatase that ... protein can then be assayed in the absence of phosphatase activity. This feature is an essential ...

Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System

... It is predicted that at least 500 kinase and phosphatase enzymes exist, their activities having wide-ranging regulatory responses. ... such as inflammation and cancer, so identifying modulators of kinase and phosphatase activity may provide new therapeutic agents.

This application ...

The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids

... or by a chemiluminescence reaction (Table 3). Of the many alkaline phosphatase substrates that can be used, we recommend CDP-Star or CSPD for ... ready to use Detection of alkaline phosphatase activity with CDP-Star results in light emission that can be recorded by ...

Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes

... 0.5% Blocking Reagent). Alkaline phosphatase conjugated antibodies The sections were incubated with anti-DIG antibody conjugated with alkaline phosphatase (Fab-fragments) (dilute 1:5001:1,000 in buffer 2) for 1 hour at room ...

Eppendorf Perfectprep Gel Cleanup Kit

... BioLabs) pUC19 DNA (New England BioLabs) Shrimp Alkaline phosphatase (Promega ) T4 DNA Ligase (New England BioLabs) TOP 10 ... was determined by A260 readings. Shrimp Alkaline phosphatase Treatment: The linearized vector was treated with SAP to ...

Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

... that transcripts for b -actin, g -actin, and protein phosphatase 1 catalytic subunit are present in libraries generated from the ... medium 449 Protein phosphatase 1 low 394 ...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

... with non-labeled ATP and for labeling of variety of double-stranded DNA ends after restriction digestion and removal of the 5'-phosphate by phosphatase treatment (data not shown). Discussion OptiKinase outperforms T4 PNK in a variety of ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

... ◊ Biotinylated rabbit-anti-rat IgG mouse-adsorbed secondary antibody (Vector Laboratories, Cat. #BA-4001) ◊ Vectastain ABC Alk- phosphatase Kit (Vector Laboratories, Cat. #AK-5200) ◊ Vector Red substrate (Vector Laboratories, Cat. #SK-5100) III. LASER CAPTURE ...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

... proteins to a nitrocellulose membrane, biotin-labeled proteins were detected by probing blots with ExtrAvidin-alkaline phosphatase (Sigma) and visualized colorimetrically by the addition of NBT/BCIP. IEF and Second-Dimension Separation of Proteins ...

Translate More Protein with Higher Biological Activity

... 5 demonstrates that a range of both prokaryotic (GroES, CAT, methionyl-tRNA transformylase (MTF), alkaline phosphatase (AP), SrmB, DEAD, and -gal) and eukaryotic (Green Fluorescent Protein (GFP) and Rhodanese (Rho)) proteins ...

Get the 5'-End

... 1 , an RNA sample containing the target of interest is treated with Calf Intestinal phosphatase (CIP) to remove the 5'-phosphate from all RNA species except those that have a cap structure (present on all Pol II transcripts). ...

Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation

... by using our high-quality Shrimp Alkaline Phosphatase. Avoid phenol:chloroform extractions; after dephosphorylation, inactivate Shrimp Alkaline phosphatase completely and irreversibly by heating at 65 C for only 15 minutes. Enjoy the convenience of a single-tube reaction; ...

Transfection Reagent Selector Kit Handbook

... hGH protein binds to the antibody on the plate, a digoxygenated antibody binds to hGH, and a secondary antibody coupled to alkaline phosphatase is used for detection. Green fluorescent protein Green fluorescent protein (GFP), originally isolated from ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... in 1983 and revolutionized transplant surgery. It works primarily by inhibiting the activity of calcineurin, a protein phosphatase regulated by intracellular calcium mobilization. All the isoforms of NFAT protein contain a calcineurin-binding domain and are ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... in 1983 and revolutionized transplant surgery. It works primarily by inhibiting the activity of calcineurin, a protein phosphatase regulated by intracellular calcium mobilization. All the isoforms of NFAT protein contain a calcineurin-binding domain and are ...

Simple Isolation of RNA from Tissue and Cultured Cells

... according to the instructions provided. The presence of PCR products for high- (-actin), medium- (g-actin) and low- (protein phosphatase 1, ADP ribosylation factors 1 and 3, and ornithine decarboxylase) abundance mRNA shows that a wide spectrum of RNA molecules is ...

New Kit Generates Exceptionally Pure Total RNA for RT-PCR

... (mouse elongation factor 1 a) , medium-abundance transcripts ( b 2-microglobulin), and low-abundance transcripts (protein phosphatase 1 and ornithine decarboxylase). Although b 2-microglobulin mRNA is classified as medium abundance, its expression level is ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... in vivo in mammalian cells. In addition, we showed that various reporter genes, such as luciferase, b -galactosidase, and secreted alkaline phosphatase can be used to detect interactions. The mammalian two-hybrid assay kit is especially useful in confirming suspected interactions between two ...
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