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Phagemid in Biological Technology

Using living cells as nanotechnology factories

... particle that infects a bacteria cell. Once inside the cell, the phagemid used the cell just like a photocopier machine to reproduce millions of copies of the DNA. By theoretically starting with just a single phagemid infection, and a single milliliter of cultured cells, Yan found that the ...

Pharmexa and Affitech to Combine Operations to Create a New Antibody Therapeutics Company

... "Affitech has developed a fully integrated antibody discovery platform consisting of human antibody libraries with high functionality, based on phagemid display technology plus several screening technologies with high degree of versatility. Through partnerships with both pharma and biotech businesses, ...

A New Lambda Vector for Mammalian Expression

... Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries ... expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector ( ... cloning site, and the pCMV-Script-EX phagemid vector. Figure ...

M13/Phagemid DNA Extraction Protocol

... agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

M13/Phagemid DNA Extraction Protocol

... agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... models the use of restriction enzymes is the blue-white color selection assay. In this assay, Stratagenes pBluescript II SK phagemid vector ( Figure 3 ), which contains the lacZ reporter gene, is cut at a single position within the multiple cloning site using ...

Purified Single-Stranded M13 DNA for Automated Sequencing

... realize high-quality automated fluorescent sequencing results as well. Additionally, the kit can be used to purify single-stranded DNA from rescued phagemid clones. REFERENCES Richards, S., et al. (1994) In Automated DNA Sequencing and Analysis (Eds. Adams, M.D., et al.) pp. 191-198. ...
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