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POL in Biological Technology

PolyOne Corporation Announces an Exclusive License Agreement with Battelle for Bio-Plasticizer Technology

... AVON LAKE, Ohio, Aug. 5 /PRNewswire-FirstCall/ -- PolyOne Corporation (NYSE: pol ), a premier provider of specialized polymer materials, services and solutions, announced today that it has entered into an exclusive license ...

Monsanto Company Signs Share Subscription Agreement With Devgen

... Officer of Devgen. "This strengthens Devgen's ability to develop and commercialize innovative biotech traits and seeds that meet farmers' needs." pol Bamelis, Chairman of Devgen, added: "We are pleased to strengthen our relationship with Monsanto. I would like to take the opportunity to acknowledge ...

More siRNA Vectors for RNA Interference

... control of the U6 promoter and several other RNA pol III promoters, it was found that ... have shown that different RNA pol III promoters generate small RNAs that ... to terminate transcription by RNA pol III. The insert design shown above is ...

Drive Long-Term Silencing with CMV Vectors

... performs better than most pol III promoters under long term ... clones is possible interference between the pol II and III promoters. ... it is hypothesized that when pol II and III promoters are in ...

Five Ways to Produce siRNAs

... in mammalian cells (14). RNA pol III was chosen to drive siRNA ... Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin ... and colleagues (5), SECs include an RNA pol III promoter, a sequence encoding an ...

GENE SILENCING WITH PCR PRODUCTS

... to a hairpin siRNA template and an RNA pol III transcription termination site. When ... cells, the SECs are transcribed by endogenous RNA pol III to generate a small hairpin siRNA ... one or more PCRs with the included RNA pol III promoter-containing DNA ...

siRNA Design Guidelines

... also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide ... as a termination signal for RNA pol III, avoid stretches of > 4 T's or A's ...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

... to be comparable for both in vitro prepared siRNAs and RNA pol III-expressed siRNAs. This is also true for chemically synthesized siRNAs versus RNA pol III-expressed siRNAs. Therefore, it is not necessary to ...

RNA Interference and Gene Silencing: History and Overview

... expression vector developed by a different research group encodes the sense and antisense siRNA strands under control of separate pol III promoters (52 ). The siRNA strands from this vector, like the shRNAs of the other vectors, have 5 ...

siRNA Expression Vectors with Selectable Markers

... repeat is complementary to the mRNA to which the siRNA is targeted. A string of thymidines added to the 3' end serves as a pol III transcription termination site. (See Design of Vector Encoded siRNAs in sidebar). Once inside the cell, the vector ...

Get the 5'-End

... Phosphatase (CIP) to remove the 5'-phosphate from all RNA species except those that have a cap structure (present on all pol II transcripts). Molecules that are dephosphorylated in the CIP reaction include rRNA, tRNA, and most importantly, fragmented ...

High-Titer Retroviral Vectors for Gene Delivery

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. 2 The gag gene encodes internal structural proteins; the pol gene encodes reverse transcriptase (RT) and integrase; and the env gene encodes the viral envelope protein, which resides on the viral surface ...

Safe Production of High-Titer Retrovirus

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. The gag gene encodes internal structural proteins, pol encodes reverse transcriptase (RT) and integrase, and the env gene encodes the viral envelope protein. The envelope protein resides on the viral ...
Other Contents
(Date:4/23/2014)... "playwright" to their resumes in an effort to aid ... ethical issues as genomic science transitions to bedside healthcare, ... , Until now the plays of Karen H. ... and by, their peers and others in the genomics ... With Oxford University Press, publication of the book ...
(Date:4/23/2014)... is available in French and German . ... female? In humans and other mammals, the difference between sexes depends ... is present only in males, where the two sexual chromosomes are ... Y is ultimately responsible for all the morphological and physiological differences ... the case. A very long time ago, the X and Y ...
(Date:4/23/2014)... most in their mid-thirties and forties, face a ... within two decades. "Mutant" protein clusters, long blamed ... been the primary focus of therapies in development ... from Prof. Gerardo Lederkremer and Dr. Julia Leitman ... and Immunology, in collaboration with Prof. Ulrich Hartl ...
Breaking Biology News(10 mins):Bioethicists use theatrical narratives to bridge the gap between society and science 2Bioethicists use theatrical narratives to bridge the gap between society and science 3Male or female? 2Male or female? 3On the defensive 2On the defensive 3
(Date:4/24/2014)... by a scientist at the University of York reveals ... the development of the nervous system may also play ... A research team, led by Dr Will Brackenbury, ... Biology at York, has studied how voltage-gated sodium channels ... are found in the membranes of excitable cells, such ...
(Date:4/23/2014)... healthy, unrelated donors was safe and effective in ... Clostridium difficile , according to a new ... and available online. Known as fecal microbiota ... via a colonoscope or a nasogastric tube. The ... treatment more readily available to patients. , A ...
(Date:4/23/2014)... say clinical trials for a new experimental drug ... promising. Patients treated with CPX-351, a combination of ... better responses than patients treated with the standard ... leukemia is an aggressive blood cancer with very ... patients," explained Jeffrey Lancet, M.D., senior member of ...
(Date:4/23/2014)... scientific director at the National Institute of Arthritis and ... 2014 recipient of the Ross Prize in Molecular Medicine, ... Molecular Medicine . The award will be given on ... in Manhattan, followed by scientific presentations by Dr. O,Shea ... National Institutes of Health. , The award, which includes ...
(Date:4/23/2014)... Va., April 23, 2014 The American Society for ... "The Role of Postoperative Radiation Therapy for Endometrial ... use of adjuvant radiation therapy in the treatment ... published in the May-June 2014 issue of ... practice journal of ASTRO. The full-length guideline is ...
Breaking Medicine News(10 mins):Health News:Breast cancer replicates brain development process 2Health News:Pilot study suggests ways to widen access to fecal transplants for C. diff infections 2Health News:Moffitt Cancer Center's phase 3 study may be game-changer for acute myeloid leukemia 2Health News:NIH scientist to receive Ross Prize in Molecular Medicine 2Health News:ASTRO issues guideline on the role of postoperative radiation therapy for endometrial cancer 2Health News:ASTRO issues guideline on the role of postoperative radiation therapy for endometrial cancer 3
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