Navigation Links
POL in Biological Technology

PolyOne Corporation Announces an Exclusive License Agreement with Battelle for Bio-Plasticizer Technology

... AVON LAKE, Ohio, Aug. 5 /PRNewswire-FirstCall/ -- PolyOne Corporation (NYSE: pol ), a premier provider of specialized polymer materials, services and solutions, announced today that it has entered into an exclusive license ...

Monsanto Company Signs Share Subscription Agreement With Devgen

... Officer of Devgen. "This strengthens Devgen's ability to develop and commercialize innovative biotech traits and seeds that meet farmers' needs." pol Bamelis, Chairman of Devgen, added: "We are pleased to strengthen our relationship with Monsanto. I would like to take the opportunity to acknowledge ...

More siRNA Vectors for RNA Interference

... control of the U6 promoter and several other RNA pol III promoters, it was found that ... have shown that different RNA pol III promoters generate small RNAs that ... to terminate transcription by RNA pol III. The insert design shown above is ...

Drive Long-Term Silencing with CMV Vectors

... performs better than most pol III promoters under long term ... clones is possible interference between the pol II and III promoters. ... it is hypothesized that when pol II and III promoters are in ...

Five Ways to Produce siRNAs

... in mammalian cells (14). RNA pol III was chosen to drive siRNA ... Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin ... and colleagues (5), SECs include an RNA pol III promoter, a sequence encoding an ...

GENE SILENCING WITH PCR PRODUCTS

... to a hairpin siRNA template and an RNA pol III transcription termination site. When ... cells, the SECs are transcribed by endogenous RNA pol III to generate a small hairpin siRNA ... one or more PCRs with the included RNA pol III promoter-containing DNA ...

siRNA Design Guidelines

... also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide ... as a termination signal for RNA pol III, avoid stretches of > 4 T's or A's ...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

... to be comparable for both in vitro prepared siRNAs and RNA pol III-expressed siRNAs. This is also true for chemically synthesized siRNAs versus RNA pol III-expressed siRNAs. Therefore, it is not necessary to ...

RNA Interference and Gene Silencing: History and Overview

... expression vector developed by a different research group encodes the sense and antisense siRNA strands under control of separate pol III promoters (52 ). The siRNA strands from this vector, like the shRNAs of the other vectors, have 5 ...

siRNA Expression Vectors with Selectable Markers

... repeat is complementary to the mRNA to which the siRNA is targeted. A string of thymidines added to the 3' end serves as a pol III transcription termination site. (See Design of Vector Encoded siRNAs in sidebar). Once inside the cell, the vector ...

Get the 5'-End

... Phosphatase (CIP) to remove the 5'-phosphate from all RNA species except those that have a cap structure (present on all pol II transcripts). Molecules that are dephosphorylated in the CIP reaction include rRNA, tRNA, and most importantly, fragmented ...

High-Titer Retroviral Vectors for Gene Delivery

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. 2 The gag gene encodes internal structural proteins; the pol gene encodes reverse transcriptase (RT) and integrase; and the env gene encodes the viral envelope protein, which resides on the viral surface ...

Safe Production of High-Titer Retrovirus

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. The gag gene encodes internal structural proteins, pol encodes reverse transcriptase (RT) and integrase, and the env gene encodes the viral envelope protein. The envelope protein resides on the viral ...
Other Contents
(Date:7/27/2014)... vitro study has revealed that nicotine and cotinine, a ... by a certain carcinogen in smoke. , The carcinogen ... tobacco leaves and ultimately ends up in the tobacco ... and liver, where it is activated by a variety ... mice has revealed that nicotine can partially interfere with ...
(Date:7/27/2014)... asteroid strike that wiped them out if it had ... say. , A fresh study using up-to-date fossil records ... a new narrative of the prehistoric creatures, demise, some ... the few million years before a 10km-wide asteroid struck ... This included extensive volcanic activity, changing sea levels and ...
(Date:7/27/2014)... to date, researchers from the U.K. provide national, regional, ... virus (HCV). Findings published in Hepatology, a journal of ... indicate that genotype 1 is the most prevalent worldwide, ... reside in East Asia. Genotype 3, at just over ... by genotypes 2, 4, 6, and 5. , ...
Breaking Biology News(10 mins):Nicotine found to inhibit DNA-strand break caused by a certain carcinogen in smoke 2Dinosaurs fell victim to perfect storm of events, study shows 2Hepatitis C virus genotype 1 is most prevalent worldwide 2
(Date:7/28/2014)... WA (PRWEB) July 28, 2014 According to ... Vkool.com, this is a comprehensive fitness guide that teaches men ... per week. , Vkool reveals in its review that ... discover:, Ways to gain the maximum ... to build muscle and avoid fat gain , ...
(Date:7/28/2014)... July 28, 2014 TherapySites, ... health professionals, announced today its affiliation with the ... This new relationship allows TherapySites to continue to extend ... benefits and promotional offers. , “There has been a ... today. With nearly 3 billion Internet users, it ...
(Date:7/28/2014)... (PRWEB) July 28, 2014 CW4K was ... First Lady Michelle Obama and Partnership for a ... as they join the group's DrinkUP effort, ... water, more often. , The seven organizations added ... Nalgene® Outdoor Products, S’well Bottle, Santa Clara Valley Water ...
(Date:7/28/2014)... July 28, 2014 Footwear etc. is ... styles from Mephisto. With shoes, sandals, and boots that ... products continually provide handmade comfort shoes and ... women have come to rely on the superb value, ... Mephisto shoes and sandals contain the highest quality materials ...
(Date:7/28/2014)... Washington, DC (PRWEB) July 28, 2014 ... beyond, many are looking to private exchanges. “Private ... the August 6 webinar from Atlantic Information Services, will ... insurers’ private exchange strategies for the future. , Barbara ... from PwC with extensive experience in the private exchange ...
Breaking Medicine News(10 mins):Health News:Greek God Muscle Building Program Review Exposes Greg O’Gallagher's Muscle Building Guide for Men – Vkool.com 2Health News:Greek God Muscle Building Program Review Exposes Greg O’Gallagher's Muscle Building Guide for Men – Vkool.com 3Health News:TherapySites (Websites for Therapists) Partners with the Illinois Association for Marriage and Family Therapy 2Health News:CannedWater4Kids (CW4K) Among Those Invited to the White House to Support First Lady Michelle Obama in Welcoming Seven New Organizations to DrinkUP 2Health News:CannedWater4Kids (CW4K) Among Those Invited to the White House to Support First Lady Michelle Obama in Welcoming Seven New Organizations to DrinkUP 3Health News:Footwear etc. Highlights Phenomenal Models of Mephisto Shoes 2Health News:Footwear etc. Highlights Phenomenal Models of Mephisto Shoes 3Health News:Three PwC Consultants to Offer Insurers Successful Strategies for Private Exchanges 2
Other TagsOther Tags