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Ovary in Biological Technology

Interim Report for the Period 1 January - 30 September 2008 (Unaudited)

... miRNA targets involved in major cancers (lung, colon, breast and ovary cancer) for our LDT's. These tests will address sub-segments of the ... drug resistance testing in major cancers including colon, lung (NSCLC), ovary and breast, where a cellular based EDR test is currently offered. The ...

Stem cells stand up for themselves

... anchor themselves in the Drosophila ovary. Previous work indicated that ovary stem cells attach to the niche through the protein E-cadherin. O'Reilly et ... the cytoskeleton. They found that follicle stem cells (FSC)one type of ovary stem celldrifted away from their niche when they carried mutant integrins. ...

Sangamo BioSciences Reports Second Quarter 2009 Financial Results

... a wholly-owned member of the Roche Group. Genentech scientists demonstrated the successful knockout of two pre-selected genes in a Chinese hamster ovary (CHO) cell line using Sangamo's proprietary zinc finger DNA-binding protein nuclease (ZFN) technology. Genentech is using ZFN technology to generate ...

SAFC Biosciences(R) Expands Media Capability for Chinese Hamster Ovary (CHO) Cell Lines

... of the Sigma-Aldrich Group (Nasdaq: SIAL ), today announced that it has expanded its portfolio of products and services for use in Chinese Hamster ovary (CHO) cell line applications with the introduction of EX-CELL(TM) CD CHO Fusion , the Company's next generation chemically defined media ...

Ferring Celebrates Winner of 2008 My Little Miracle Essay Contest During National Infertility Awareness Week

... pituitary or hormone dependent tumor, abnormal uterine bleeding of undetermined origin, or ovarian cysts or enlargement not due to polycystic ovary syndrome. Like all gonadotropins, these are potent substances capable of causing mild to severe adverse reactions, including Ovarian Hyper ...

KGI Offers Mammalian Cell Culture Program for Biotech Industry Professionals

... to become more knowledgeable, operationally adept and business savvy about this growing field." According to Croughan, recombinant Chinese hamster ovary (CHO) cells now surpass E Coli and all other expression systems for recombinant protein production, with total annual sales exceeding $30 billion. ...

Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options

... we believe physicians will be able to quickly direct ovarian cancer patients towards appropriate lines of therapy." Epithelial carcinoma of the ovary is one of the most common gynecologic malignancies and the fifth most frequent cause of cancer death in women. In 2008, ovarian cancer will be ...

SurgiQuest Announces First Single Port Procedure for Ovary Removal Using AirSeal(TM) Access System

... ORANGE, Conn., May 7 /PRNewswire/ -- SurgiQuest, Inc. today announced the first single port laparoscopic removal of an ovary using the AirSeal(TM) access system. Drs. Anthony Discuillo and Christopher Awtry performed the procedure at Mount Auburn Hospital in Cambridge, ....

Surface Logix Achieves Objectives With SLx-4090 in Phase 2a Clinical Trial

... inhibits fat and cholesterol uptake but avoids the mechanistic toxicities of MTP inhibition in the liver and other organs such as the heart, testis, ovary and eye. Surface Logix is also exploring the use of SLx-4090 in other metabolic disorders, including obesity and diabetes. Dyslipidemia currently ...

InVitria's Animal Free Lacromin(TM) Provides a Cost-Effective Improvement Over Transferrin

... media ingredients: -- Strong growth factor Lacromin(TM) has been shown to promote cell growth in many cell lines, including Chinese Hamster ovary (CHO) cells and Mouse Hybridoma cells. -- Outperforms Transferrin Lacromin(TM) promotes higher cell density and reduces cell doubling time ...

Novare Announces First Ever Single Port Laparoscopic Kidney Removal (Nephrectomy) Using RealHand(TM) HD Instruments

... trauma was consolidated to a very small incision." These cases follow announcements of gall bladder removals, total laparoscopic hysterectomies and ovary removals being performed utilizing the Single Port Access technique first developed at the Drexel University College of Medicine. "With Dr. Cadeddu's ...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

... of [ 35 S]-GTPγS binding in Chinese hamster ovary cells. Results are compared to those obtained ... 5-HT 1B receptors expressed in Chinese hamster ovary (CHO) cells. Methods Receptor Source A Chinese hamster ovary cell line (ACC098) expressing the human 5-HT 1B ...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

... CyBi™-well (Cy-Bio) Chinese hamster ovary (CHO-K1) cells Isoproterenol ... cells established in culture were carried out as follows: Chinese hamster ovary (CHO-K1) cells were seeded (2 µl, 10 6 cells/ml) into 1536-well ...

Protect RNA and Improve Target Cell Identification

... These representative photographs show an 8 m section from human ovary (20X magnification) that was stained with Cresyl Violet and ... HPRT mRNA was performed with total RNA isolated from human ovary LCM sample after staining with Cresyl Violet (see Figure 2; LCM ...

UW-Madison scientists find a key to cell division

... postdoctoral researcher and now at the University of Minnesota, and Michael Mullins, now at Catholic University, to isolate midbodies from hamster ovary cells. They then analyzed and identified more than 500 proteins contained in the midbodies. "Proteins are the building blocks of the cell," Skop ...

The birth of biotech: San Francisco, Boston, Geneva or Chicago?

... Lin spent two years working out the process for sequencing the EPO protein and another year to sequence and clone the process using Chinese hamster ovary cells. Lins first patent was filed on Dec. 13, 1983 with other patents filed on the process for producing recombinant EPO soon thereafter. During ...

Improving the accuracy and speed of mammalian cell counting

... behind the NucleoCounter ( New Brunswick Scientific Co., Inc. , Edison, NJ; figure 1) and its operation, including data from a run of Chinese hamster ovary (CHO) cells. Alternate counting methodologies, both manual and automated, are also discussed. Today, most laboratories count cultured cells ...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

... 4 , 6773. APPENDIX Materials Cells : CHO cells (M1-WT3) - adherent Chinese Hamster ovary cells stably expressing the rM 1 -muscarinic receptor (ECACC Ref. #: 940822109) Reagents : FlexStation Calcium Assay Kit ...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

... 1. LIVE/DEAD Viability/Cytoxicity Kit (Molecular Probes, cat# L-3224) Tel: 1-541465-8300 2. Chinese Hamster ovary (CHO) cells 3. 96 well black microtiter plates with clear bottoms (Costar, cat# 3603). Tel: 1-800-4921110 4. PBS (Phosphate ...

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

... Methods Generation of cAMP Source The method is an adaptation from McHale, et al .1 Chinese Hamster ovary cells expressing human 5-HT 1D receptors were seeded and grown to confluence. They were subsequently harvested and resuspended in medium containing ...

Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors

... Kasila). We have investigated the use of this novel FlashPlate technology to measure the activation of adenylyl cyclase in Chinese Hamster ovary (CHO) cells expressing human -2 adrenoreceptors (h-2 receptors). Materials Adenylyl Cyclase Activation FlashPlate Assays ...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

... cAMP – [ 125 I] Direct Biotrak™ SPA Screening Assay (RPA 559).

Method Chinese hamster ovary (CHO) or A431 cells were seeded into 384-well cluster plates suitable for both cell culture and imaging. Cells were stimulated (20 min 37 °C, 5% ...

SUPERIOR RNA FOR DEMANDING APPLICATIONS

... of total RNA isolated from an individual tumor and the normal adjacent tissue (NAT) (Note: Due to the nature of the samples, ovary and lymphoma NATs are not always from the same individual as the tumor tissue). Because of the small size of each ...

Detecting miRNA & siRNA

... Interestingly the mir-22 probe showed a completely different pattern of expression. miR-22 was highly expressed in lung and ovary and was present in spleen, thymus, and testicle at levels that would not have been detectable with standard Northern blotting techniques ...

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

... Discussion Recombinant human tissue plasminogen activator (rt-PA) was expressed in Chinese hamster ovary cells and purified. This glycoprotein has an approximate molecular weight of 64 kDa and a heterogeneous distribution of N-linked ...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

... Chinese Hamster CHO: ovary cell line Drosophila L2: Schneider cells Fungi ...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

... Chinese Hamster CHO: ovary cell line Drosophila L2: Schneider cells Fungi ...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

... All constructs were tested and confirmed for specificity and activation levels in transient transfection assays in Chinese hamster ovary (CHO) and HeLa cells (unpublished data). We also cloned the activation domains of the ATF2 and c-Fos transcription factors into the pFA-CMV ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... expression level of the pDual GC vector, firefly luciferase activity was measured in transiently transfected Chinese hamster ovary (CHO) cells. Luciferase was chosen because it can be assayed both enzymatically and immunologically. Results of the enzymatic luciferase ...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

... activated by IFN- g . Both the pISRE-Luc and pGAS-Luc plasmids functioned in other cell lines as well. Luciferase expression in Chinese hamster ovary (CHO) cells transfected with the pISRE-Luc plasmid, for example, increased by over 8-fold after treatment with 2,000 U/ml IFN- b (data not ...

Mammalian Expression Vectors with Interchangeable Selectable Markers

... Selectable Cassettes To test the performance of the eukaryotic selectable markers provided by each cassette, we transfected Chinese hamster ovary (CHO) and NIH3T3 cells with the core vector containing the selectable marker that was introduced by site-specific recombination. Each construct ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... cloned into the pCMV-Script vector, and the resulting construct was transfected using standard lipid transfection methods into Chinese hamster ovary (CHO) cells. The expression of the GFP gene product was detected by Western blot analysis (data not shown). Figure 2 ...

A New Lambda Vector for Mammalian Expression

... was inserted into the parental pCMV-Script vector. These two constructs were used for parallel transfections of Chinese hamster ovary (CHO) cells. Cell lysates were prepared and assayed for luciferase activity. In Figure 4 , cells transfected with each ...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

... it can be assayed both enzymatically and immunologically. This construct was transiently transfected into Chinese hamster ovary (CHO) cells, and the cell lysates were assayed for luciferase activity. The results of these luciferase assays (figure ...
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