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Nuclease in Biological Technology

Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations

... of Transgenomic's WAVE DHPLC and Surveyor nuclease products for mutation detection in cancer and ... that coupling Cold-PCR with DHPLC and Surveyor nuclease promises a significant solution to high ... an alternative offering, Transgenomic's SURVEYOR nuclease cleaves such heteroduplexes with high specificity ...

Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology

... key advantages of using Transgenomic's Surveyor nuclease technology for mutation scanning of PKD1 and ... of scanning technology based upon Surveyor nuclease and the WAVE System." said Dr. Eric Kaldjian, CSO ... by DNA sequencing were shown by Surveyor nuclease to be heterozygous." ADPKD occurs at a rate ...

Sigma-Aldrich Launches Breakthrough Genome-Editing Tools

... CompoZr(TM) Zinc Finger nuclease Platform enables Precise, Permanent, and ... launched its CompoZr(TM) platform of zinc finger nuclease (ZFN) reagents. The CompoZr ZFN platform of ... including rats and rabbits. "Zinc Finger nuclease technology has held significant promise for ...

Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market

... for Technology Innovation of the Year. The company's radical Directed nuclease Editor(TM) (DNE) technology allows it to target and modify regions of a ... the field of genomic molecular biology. Precision's proprietary Directed nuclease Editor(TM) (DNE) technology enables the production of custom genome ...

Sangamo BioSciences Reports Fourth Quarter and Full Year 2008 Financial Results

... subjects. Based on Sangamo's zinc finger DNA-binding protein (ZFP) nuclease (ZFN(TM)) technology, SB-728-T has been shown to lead to an increase in ... Roche with non-exclusive, worldwide rights for the use of Sangamo's ZFP nuclease (ZFN(TM)) technology to develop cell-lines and transgenic animals with ...

Sangamo BioSciences Reports Third Quarter 2008 Financial Results

... Official launch of Sigma-Aldrich CompoZr(TM) platform of zinc finger nuclease (ZFN) reagents for gene-editing. In September, Sangamo's partner, ... pathways. For more information on Sigma-Aldrich's CompoZr zinc finger nuclease technology platform, visit http://www.compozrzfn.com or contact a ...

Silence Therapeutics Receives Allowance from United States Patent and Trademark Office for its Core RNAi Patent

... over conventional siRNA molecules, including increased stability against nuclease degradation and an increased serum half-life, while retaining therapeutic ... over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic ...

Sangamo BioSciences and Sigma-Aldrich Announce Research and License Agreement With Roche for the Use of ZFN Technology for Generation of Transgenic Animals

... non-exclusive, worldwide rights for the use of its proprietary zinc finger nuclease (ZFN) technology to develop cell-lines and transgenic animals that have ... genes of interest. ZFNs are engineered forms of ZFPs that also contain a nuclease component which can facilitate modification of a target gene of interest. ...

Sangamo BioSciences Reports Second Quarter 2009 Financial Results

... successful knockout of two pre-selected genes in a Chinese hamster ovary (CHO) cell line using Sangamo's proprietary zinc finger DNA-binding protein nuclease (ZFN) technology. Genentech is using ZFN technology to generate cell lines with novel characteristics for pharmaceutical protein production purposes. ...

OMT announces a breakthrough in the development of a novel human antibody platform

... is essential for human antibody expression in genetically engineered animals. To solve this problem, we explored a new application for Zinc Finger nuclease (ZFN) technology, which enabled a technique that could revolutionize the genetic engineering of animals." In the study, OMT scientists and its ...

Sigma-Aldrich Streamlines Access to Advanced Stem Cell Research Portfolio and Opens Stem Cell Biology Community Through New Web Portal

... the MISSION(R) RNAi portfolio, which enables gene silencing across a wide range of cellular targets. The site also features the CompoZrTM zinc finger nuclease (ZFN) reagent platform which enables researchers, for the first time, to target and precisely manipulate the genome of both differentiated cells and ...

Sigma-Aldrich (Nasdaq: SIAL) Reports Fourth Quarter Diluted EPS of $0.68. Ends 2008 With Record Sales and Earnings and Raises Quarterly Cash Dividend by 11.5%. Provides Outlook for 2009.

... with 70 new technology in-licenses in 2008 bringing total in-licenses held to over 600. -- Received recognition for CompoZr Zinc Finger nuclease (ZFN), a breakthrough gene editing technology, as one of the "Top 10 Innovations of 2008" by The Scientist magazine. 2009 Outlook on Sales ...

Sangamo BioSciences Initiates Phase 1 Trial of CCR5-ZFP Therapeutic to Treat HIV/AIDS

... have opened a Phase 1 clinical trial to evaluate SB-728-T for the treatment of HIV/AIDS. Based on Sangamo's zinc finger DNA-binding protein nuclease (ZFN) technology, SB-728-T has been shown in an animal model of HIV infection to lead to an increase in CD4+ T-cell counts, a reduction in viral ...

Sigma-Aldrich and Sangamo BioSciences Announce the Achievement of Key Throughput Milestone in Research Reagents Agreement

... reached a major production capacity milestone as part of its agreement with Sigma-Aldrich Corporation (Nasdaq: SIAL ) to commercialize zinc finger nuclease reagents (ZFNs) for research applications. The milestone triggers a payment of $1.0 million to Sangamo. "Reaching this production milestone more ...

Transgenomic Reports Second Quarter 2008 Results

... Libraries (SEAL). This cost-effective and high-throughput enabling technology for whole genome analysis evolved from our highly sensitive SURVEYOR nuclease mutation detection technology. SEAL will prove extremely useful for whole genome sequence analyses and a host of key mutation and variation analyses ...

Silence Therapeutics Patent Update

... RNA ('siRNA') molecules, AtuRNAi, which provide a number of advantages over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic delivery system, AtuPLEX. This system enables the functional delivery of ...

Silence Obtains Important Patent Protection for Lead Internal Development Compound, Atu027

... RNA ('siRNA') molecules, AtuRNAi, which provide a number of advantages over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic delivery system, AtuPLEX. This system enables the functional delivery of ...

Organizational Changes at Silence Therapeutics

... RNA ('siRNA') molecules, AtuRNAi, which provide a number of advantages over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic delivery system, AtuPLEX. This system enables the functional delivery of ...

Silence Therapeutics Announces Successful Opposition of Glover Patent

... RNA ('siRNA') molecules, AtuRNAi, which provide a number of advantages over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic delivery system, AtuPLEX. This system enables the functional delivery of ...

FDA Approves Quark IND for DGFi, an siRNA Therapeutic Based on Silence Therapeutics' Unique Proprietary Chemistry

... RNA ('siRNA') molecules, AtuRNAi, which provide a number of advantages over conventional siRNA molecules, including increased stability against nuclease degradation. In addition, the Company has developed a proprietary systemic delivery system, AtuPLEX. This system enables the functional delivery of ...

Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... that are subsequently hybridized to generate mismatches in heteroduplexed DNA which is then treated and cleaved by SURVEYOR Nuclease. SURVEYOR nuclease can detect genetic variations in samples with high sensitivity. About Transgenomic Transgenomic (OTC Bulletin Board: TBIO) is a global ...

Sangamo BioSciences Announces License Agreement For the Use of ZFP Technology For Generation of Transgenic Animals

... OMT Product, OMT has the right to buy out its future royalty payment obligations under the Agreement by paying a lump sum fee to Sangamo. "Our ZFP nuclease (ZFN) platform addresses a major problem that has long hampered the development of transgenic models in species other than mice," said Philip ...

Sangamo BioSciences Announces Expansion of Research and License Agreement With Genentech for ZFP Technology for Protein Pharmaceutical Production

... to include additional targets for potential improvement of production cell lines using Sangamo's proprietary zinc finger DNA-binding protein nuclease (ZFN) technology. "We are pleased to expand our relationship with Genentech, a company that has always operated at the cutting edge of innovative ...

Dow AgroSciences, Sangamo BioSciences Announce the Achievement of Key Milestone in Plant Agriculture Collaboration

... joint Research and Commercial License Agreement. This milestone represents the successful application of Sangamo's zinc finger DNA-binding protein nuclease (ZFN(TM)) technology in precision genome editing of a native plant gene with the aim of improving a valuable trait in canola, a crop central to Dow ...

DuPont and Precision BioSciences Collaborate to Accelerate Crop Product Development

... the market faster. Terms of the agreement were not disclosed. The agreement provides DuPont business Pioneer Hi-Bred rights to Precision's Directed nuclease Editor(TM) (DNE) technology to introduce and stack multiple genes that accelerate product development in corn, soybeans and other important ...

Enigma Signs Real-Time PCR Licences With Applera

... Kit License Agreement covers a portfolio of important patents covering reverse transcription-based methods, real-time PCR detection process and 5' nuclease assays and compositions, for use in fields excluding human diagnostics. Financial terms were not disclosed. John McKinley, Chairman of Enigma ...

RNA Interference and Gene Silencing: History and Overview

... RNA-induced silencing complex. A nuclease complex, composed of proteins and ... step, the siRNA duplexes bind to a nuclease complex to form what is known as the RNA-induced ... encodes a protein with homology to the nuclease domains of RNase D and a protein implicated in ...

Nuclease Protection Assays: The Basics

... What is a nuclease Protection Assay? nuclease protection assays (NPAs), including both ribonuclease protection assays (RPAs) and S1 nuclease assays, are an extremely sensitive ...

Northern Analysis: The Basics

... gene array analysis and nuclease protection assays. Northern ... general, less sensitive than nuclease protection assays and RT-PCR, although ... the sensitivity of Northerns to the level of nuclease protection assays. A third ...

SNPs Powerful Tools for Association Studies

... In addition, because most SNPs are biallelic and technologies such as 5' nuclease assays enable one-step genotype calling, SNPs are also the easiest genetic ... two pairs of each chromosome. Genotyping methods (including TaqMan 5' nuclease assays and direct sequencing) detect both alleles simultaneously, yielding ...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

... 96-well reaction plates containing reagents, standards and controls for 5' nuclease assay analysis or other nucleic acid-based assays. ... can then be prepared, containing standards, controls, reagents for 5' nuclease assay or other nucleic acid-based assay reagents. A sample of the ...

Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

... times with sterile PBS, 200 ml/well.

8. Reconstitute nuclease reagent with 26 ml of sterile water. To this, add 260 ml of anti-BrdU ... for 45 min at 37 °C in 5% CO 2 .

10. Discard nuclease reagent and wash the cells three times with sterile PBS, 200 ml/well. ...

Which Water to Use?

... nuclease-free, each lot of water is tested for nuclease activity by incubating a sample overnight with a ... with 18S rRNA primers. Each lot is also tested for nuclease contamination. This product is ideal for use in any ...

Measuring RNase Activity A Real-time Kinetic Analysis

... mung bean nuclease, and Benzonase. In addition to detecting nuclease activity, this kit is useful for standardizing activities ... recommended when the absence of DNase and other nonspecific nuclease activities is essential. Measuring Specific Activity ...

Confirming RNAi Effects Is Now Easier Than Ever

... siRNA transfection, including Northern analysis, nuclease protection assay, gel-based RT-PCR, and real-time ... of Applera Corporation. The PCR process and 5' nuclease process are covered by patents owned by Roche ...

Evaluation of FlashPlate in a Helicase Assay

... of which demonstrated that decreases in CPM were specific to T-Antigen helicase activity, and not a consequence of temperature-induced melting or nuclease activity (Figure 2). Furthermore, the level of DNA unwinding activity was shown to be dependent upon the dosage of T-Antigen added (Figure 3). ...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

... ◊ RNase AWAY (Life Technologies, Cat.# 10328-011) ◊ Detergent (Fisher Scientific, Cat. #04-355) ◊ Pipette Tips, nuclease Free ◊ 0.5 mL or 0.2 mL RNase-Free ◊ Microcentrifuge Tubes ◊ 2 mL lidless Tube (PGC Scientific, Cat. # ...

Oragene is compatible with TaqMan SNP genotyping

... addition to diagnostic applications, SNPs are useful as markers in population genetics and evolutionary studies (ref. 2). The TaqMan 5 nuclease assay is a widely-used SNP genotyping technology from Applied Biosystems. The purpose of this study was to evaluate the ...

The iCycler iQ Detection System for TaqMan Assays, Rev B

... 986994 (1996) Holland PM et al., Detection of specific polymerase chain reaction product by utilizing the 5'→3' nuclease activity of Thermus aquaticus DNA polymerase, Proc Natl Acad Sci USA 88, 72767280 (1991) * Practice of the patented ...
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