Mycoplasma in Biological Technology

New White Paper Examines Advanced Mycoplasma Tests - How Real-Time PCR Analysis Helps Save Time and Money for Biopharmaceutical Manufacturers

... detect. A new free white paper, examines how PCR mycoplasma tests and analysis , deployed by a cutting-edge ... Manufacturers ," explores relevant mycoplasma characteristics, established testing solutions, ... alternative testing method for fast, preliminary mycoplasma detection . It also available for download ...

J. Craig Venter Institute Researchers Publish Significant Advance in Genome Assembly Technology

... can now assemble the whole bacterial genome, mycoplasma genitalium, in one step from 25 fragments of ... techniques used to change one bacterial species, mycoplasma capricolum, into another, mycoplasma mycoides Large Colony (LC), by replacing one ...

Venter Institute Scientists Create First Synthetic Bacterial Genome

... synthesizing and assembling the 582,970 base pair genome of a bacterium, mycoplasma genitalium JCVI-1.0. This work, published online today in the journal ... outlined the methods and techniques used to change one bacterial species, mycoplasma capricolum, into another, Mycoplasma mycoides Large Colony (LC), by ...

Microtest Labs Expands Clean Room, Contamination Testing Services

... microbial identification utilizing in-house MicroSeq(R) Microbial Identification Systems for fast, precise and reliable bacteria, mycoplasma and mold identification. Compressed air/gas testing , including nonviable air particulate testing; compressed ...

Mediatech Moves to Prince William County Innovation Park

... and meets the most rigid Sterility Assurance Levels for liquid products. All cellgro(R) basal media and salt solutions must test negative for mycoplasma prior to release. For more information visit http://www.cellgro.com . Contact: Julie Fry, International Marketing Associate I, Mediatech, Inc. ...

The QIAGEN Guide to Animal Cell Culture

... used against bacteria and fungi. Furthermore, as mycoplasma do not overgrow cell cultures and ... There are two main approaches to detect mycoplasma Hoechst 33258 staining (1, 2) and ... in a separate room. Elimination of mycoplasma is commonly achieved by treatment with various ...

Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells

... mycoplasma PCR Primer Set for fast and easy detection of ... LLC Heinz Miller Stratagene mycoplasma contamination of mammalian cell cultures can ... accurately determined. The use of Stratagenes mycoplasma PCR Primer Set *, provides assurance that ...

Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

... reagents. In addition, we examine the effect of mycoplasma contamination of cell lines on transfection ... lines. Transfection Efficiencies: Effect of mycoplasma Contamination When standardization studies ... may be observed. This may be due to mycoplasma contaminating the cell lines, since ...

A New PCR-based Mycoplasma Detection Method

... Reliable, PCR-based mycoplasma detection Douglas McKenzie Heinz Miller ... Stratagene Stratagene introduces the mycoplasma PCR Primer Set Plus, a kit that uses a ... sensitive means to determine information about mycoplasma (and related acholeplasma) species. The kit ...

Uses and Applications of FuGENE 6 Transfection Reagent

... The culture may be contaminated with mycoplasma Use the mycoplasma Detection Kit (Cat. No. 1 296 744) or mycoplasma PCR ELISA (Cat. No. 1 663 925) to determine if ...

Transfection Reagent Selector Kit Handbook

... between experiments. Possible mycoplasma contamination mycoplasma contamination influences transfection efficiency. ...

TransMessenger Transfection Reagent Handbook

... transfection. Possible mycoplasma contamination mycoplasma contamination influences transfection efficiency. ...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

... (DMSO) were used as negative controls. Fig.4 For the final testing, we confirmed purity by screening for mycoplasma and established viability after freezing in liquid nitrogen. The HLR, HLR-CREB, HLR-Elk1, and HLR-cJun cell lines are available as frozen stocks ...