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Mutational in Biological Technology

Gleevec Receives FDA Priority Review as First Therapy to Reduce Recurrence of Gastrointestinal Stromal Tumors After Surgery

... factor receptor) gene rearrangements; adult patients with aggressive systemic mastocytosis (ASM) without the D816V c-KIT mutation or with c-KIT mutational status unknown; adult patients with hypereosinophilic syndrome (HES) and/or chronic eosinophilic leukemia (CEL) who have the FIP1L1- PDGFR alpha ...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

... the well-characterized bacterial lacI gene as a mutational target gene. The lacI gene, part of ... number of mutants have been sequenced, a mutational spectrum results. Mutational spectra are ... the mutants revealed a unique difference in mutational spectrum: The spectrum from the liver was ...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR mutagenesis kit produces a unique mutational spectrum ... mutations with minimal bias, and its mutational spectrum is distinct from that of Taq ... in studies employing lacZ as the mutational target gene. A 650-bp DNA fragment containing a ...

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

... now offers an alternative way to perform mutational analysis in the Big Blue transgenic rodent ... studying tissue-specific mutant frequencies and mutational spectra. figure 1 ... l LIZ shuttle vector, functions as a mutational test sequence. Rescued shuttle vector ...

Organize Your Data from the Big Blue Mutation Assay

... The report compares mutant frequencies from two treatment groups. mutational events are assessed using the Mutational Spectrum Report, which tabulates mutational spectra based on the occurrence of each mutation type. Specialized ...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

... Gerdes and Kaether 2 ). Since then, a rapidly increasing variety of applications for GFP and its derivatives have been developed. mutational studies of the chromophore and surrounding amino acids have produced new GFPs with altered excitation and emission spectra. ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... deficiency, Hum. Mutat., 5 , 12630 (1995). 3. Wedell, A., Thilen, A., Ritzen, E. M., Stengler, B., and Luthman, H., mutational spectrum of the steroid 21-hydroxylase gene in Sweden: implications for genetic diagnosis and association with disease manifestation, ...

Transfection Reagent Selector Kit Handbook

... eukaryotic cells has become a powerful tool for the study and control of gene expression, e.g. for biochemical characterization, mutational analyses, or investigation of the effects of regulatory elements or cell growth behavior. Two principally different transfection ...

Mx4000 Multiplex Quantitative PCR System*,PPP

... price. Real-time quantitative PCR (QPCR) allows researchers to quickly and easily quantify nucleic acids for studying gene expression, mutational analysis, disease state, and gene dosage. QPCR measures PCR product accumulation during the exponential phase of the reaction using fluorescence ...

Map and Link Human Genetic Disorders with SSLP Analysis

... the rapid identification of new disease genes. Recognition of the heterogeneity of genetic diseases, the allelic nature of certain disorders, and mutational heterogeneity in the cause of genetic conditions may ultimately lead to understanding the relationship between phenotype and gene. Simple ...

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

... enhancing factor on the replication fidelity of Pfu DNA polymerase was investigated using the lacI-based PCR assay. The lacI mutational target gene was amplified from varying amounts of plasmid DNA and from a single-copy transgene in rodent genomic DNA, and error ...
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