JCI online early table of contents: March 6, 2008
... the SBDS protein was found to be located at the mitotic
spindle, a structure that forms when a cell is ... of a cell. Consistent with a role for SBDS in mitotic
spindle stability, BMSCs from individuals with ... the treatment of individuals with SDS.
spindle destabilization and genomic instability ...
Nerviano Medical Sciences Receives Approval From the FDA to Enter Phase I Clinical Trials for Their Polo Like Kinase (PLK) Inhibitor
... Nerviano Medical Sciences to
begin a phase I clinical study with its selective PLK-1 small molecule
inhibitor for the treatment of cancer. PLK-1 is a mitotic
kinase required for
the proliferation of cancer cells. This new compound is orally bioavailable,
highly efficient and well tolerated in preclinical ...
Neuralstem Receives Final Notice of Allowance for New Stem Cell Immortalization Technology Patent
... this patent allows us to grow practically unlimited quantities of neural stem cells from all regions of the brain without regard to the natural mitotic
(growth) limits of cells from a particular region. Equally important, this technology is a next-generation immortalization process that avoids the ...
RNAi Helps Illuminate the Inner Workings of the Cell Cycle: Synthetic RNAissance™ Genes Created by DNA2.0 Enable Key Research
... group was able to show that ubiquitination of Cdc20 is required to prevent a cell from dividing before all of its chromosomes are lined up on the mitotic
spindle. This cell cycle checkpoint is essential to prevent serious genetic damage in animals from yeast to humans.
"We were delighted to ...
EntreMed's MKC-1 Meets Primary Efficacy Endpoint in Non-Small Cell Lung Cancer Clinical Trial
... tumor growth
inhibition or regression in multiple preclinical models, including
MKC-1 has been shown to inhibit mitotic
spindle formation, prevent
chromosome segregation in the M-phase (mitosis) of the cell cycle, and
induce apoptosis. Furthermore, MKC-1 inhibits the ...
Cephalon Receives FDA Approval for TREANDA, a Novel Chemotherapy for Chronic Lymphocytic Leukemia
... leads to cell death by a process known as
apoptosis (programmed cell death) as well as by an alternate cell death
(non-apoptotic) pathway known as mitotic
catastrophe (a disruption of
normal cell division). The exact mechanism of action of TREANDA remains
In December 2007, Cephalon submitted ...
Cephalon Submits New Drug Application for TREANDA for the Treatment of Patients with Relapsed Indolent Non-Hodgkin's Lymphoma
... leads to cell death by a process known as apoptosis
(programmed cell death) as well as by an alternate cell death
(non-apoptotic) pathway known as mitotic
catastrophe (a disruption of
normal cell division). The contribution of the purine analog to the
antitumor effect of TREANDA is under investigation ...
Mouse Brain or Impressionist Painting?
... and Imaging Research at UCSD for his photos of rat
tongue and cerebellum; Pat Wadsworth of the University of Massachusetts for
her depiction of the mitotic
spindle in a diving cell; Earl Nishiguchi of
the University of Hawaii for his fruit fly testis image; Jan Schmoranzer of
Columbia University for his ...
Cephalon Submits New Drug Application for TREANDA for the Treatment of Chronic Lymphocytic Leukemia
... rapid, sustained single-
and double-strand DNA damage, which results in apoptosis, or programmed
cell death, in the tumor. TREANDA also induces mitotic
inhibition, which results in non-apoptotic cell death. TREANDA causes DNA
disruption that is both extensive and durable exhibiting a low ...
FDA Grants Orphan Drug Designation to TREANDA, an Investigational Treatment for Chronic Lymphocytic Leukemia
... rapid, sustained single- and
double-strand DNA damage, which results in apoptosis, or programmed cell
death in the tumor. TREANDA also induces mitotic
which results in non-apoptotic cell death. These novel dual-action,
anti-tumor effects of TREANDA may be attributed to its ...
Amnis Announces the Release of Multiple Upgrades to the ImageStream(R) System
... all three lasers to be captured simultaneously across the
entire visible spectrum. Customers who purchase the 405nm laser can now
perform cell cycle, mitotic
analysis and other experiments incorporating
the widely-used DNA binding dyes DAPI and Hoechst. The 658nm red laser
provides similar advantages for a ...
Quantitative Analysis of the Cell Cycle in Imaging Applications
... two major stages, interphase (the phase between mitotic
events) and mitosis. There are three distinct, ... site on the histone H3 coupled with mitotic
chromosome condensation . J. Biol. Chem 274, ... of interphase cells and at the periphery of the mitotic
chromosomes in a process essential for cell cycle ...
IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
on cell proliferation and mitotic
index (Figure 4). As seen in this example,
on cell proliferation and the mitotic
index, indicating the importance
human kinases. Cell number and mitotic
index were measured 48 hours post
Assessing Gene Function with siRNA Libraries
... their effects on cellular proliferation and mitotic
index. This screen ... expected to yield a clear decrease
index in this study. The mitotic
cells. The extent of cell ...
Functional Cloning Using
ViraPort Retroviral cDNA Expression Libraries... complex cDNA libraries to be efficiently introduced into
virtually any mitotic cell type for screening based on gene function. The cDNA
copy number per ... as
10A1 and VSV-G, will allow functional screening in essentially any mitotic cell
type. 2 Finally, the relatively high titers and high expression ...
Safe Production of High-Titer Retrovirus... transduction
efficiencies of greater than 90% are achievable for most mitotic cell
types, and the copy number per cell can be easily controlled by ... is present on all cell types, which theoretically allows any mitotic cell
to be efficiently infected. Additionally, VSV-G confers stability, ...
A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity... shifts the equilibrium in cells towards microtubule assembly, disrupting the normal operation of the microtubule network and thereby arresting the mitotic process.
Treatment with paclitaxel alone, however, is effective in only a fraction of the population. Approximately 21% to 24% of ...
siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)... and induce apoptosis (15). Cell cycle analysis (Fig 6, PLK) of G2/M CCPM cells treated with siRNA directed against PLK showed a dramatic increase in mitotic cells 48 h after transfection with 50 nM siRNA (Fig 8B). Extreme sensitivity to PLK knockdown was confirmed by analysis of G1/S CCPM (Fig 8A) and ...
Transgenic Zebrafish by Electroporation... Though it is unlikely that plasmid DNA (most in the linear form) could
be carried over in the animals cells through many rounds of mitotic division,
it is a possibility. Future experiments are planned using gel-purified
linear fragments of various constructs to try ...
Transformation of Filamentous Fungi by Microprojectile Bombardment... density,
helium pressure, and size of microprojectiles on transformation frequency
were investigated. Transformation frequency, mitotic stability of transformants,
and Southern blot hybridization patterns were compared between conidia
transformed by particle ...
The Bio-Rad Silver Stain, Rev E... et al., Gel protein stains: silver stain, Methods Enzymol
104, 441447 (1984)
58. Miake-Lye R and Kirschner MW, Induction of early mitotic events in
a cell-free system, Cell 1, 165175 (1985)
59. Miyajima A et al., Secretion of mature mouse interleukin-2 by ...
Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C... the degree of nuclear polymorphism,
nuclear to cytoplasmic ratio, and chromatin clumping, as well as the number
of mitotic cells (Friedell et al. 1983).
To achieve a more reliable and objective classification of bladder SCCs
that may eventually pave ...
hNT BlueNeurons Express b-Galactosidase at High
Levels... 6A &6B
6 A shows transfected clone B6 replate 3 neurons after 6 weeks of RA
induction, treatment with mitotic inhibitors and purification (figure
1). This photomicrograph shows two clusters of neurons that are stained
intensely blue. ...
Versatile Reporter Vectors for Monitoring Viral Transduction... cDNA libraries, part of our
growing collection of ViraPort retroviral premade libraries, are easily and
efficiently introduced into virtually any mitotic cell type for screening based
on gene function. The insert capacity of pFB, our MMLV-based
replication-defective pFB vector, is approximately 8.0 ...