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Mitosis in Biological Technology

EntreMed ENMD-2076 Phase 1 Data to Be Presented at ASCO Annual Meeting

... profile of angiogenic tyrosine kinase targets in addition to Aurora A kinase and other oncogenic proteins. Aurora kinases are key regulators of mitosis (cell division), and are often over-expressed in human cancers. ENMD-2076 targets a defined set of kinases, including Flt-3 and FGFR3, which have ...

EntreMed Initiates ENMD-2076 Clinical Trial in Multiple Myeloma

... profile of angiogenic tyrosine kinase targets in addition to Aurora A kinase and other oncogenic proteins. Aurora kinases are key regulators of mitosis (cell division), and are often over-expressed in human cancers. In addition, ENMD-2076 is relatively selective for the Aurora A isoform in comparison ...

Assessing Gene Function with siRNA Libraries

... to monitor the percent of cells undergoing mitosis at any given point in time, with and ... the fraction of time that cells spend in mitosis versus the rest of the cell cycle. ... with a control scrambled siRNA, undergoing mitosis (mitotic index range of 2.1 to 2.3). ...

Quantitative Analysis of the Cell Cycle in Imaging Applications

... commit to DNA synthesis (S phase) and replicate their chromosomal DNA. The G2 phase (second gap) follows when cells continue to grow and prepare for mitosis (M phase, division). The G2 gap allows time for the cell to complete its DNA replication before initiating mitosis. During mitosis, the ...

Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

... by their pink nuclei.

Figure 2 depicts the effect of Mitomycin C (MMC), a cytostatic drug that inhibits mitosis causing cells to accumulate in G2 phase of the cell cycle. Cells were incubated with increasing concentrations of MMC (as indicated below each set of ...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

... cells with micronuclei. As cells are exposed to higher doses of compounds, cell cycle inhibition and cytotoxicity results in cell arrest prior to mitosis preventing micronuclei formation, with a resulting drop in micronuclei frequency at higher compound doses. This is evident from examination of ...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

... gene knockdown. For example, treatment of cells with cyclin A2 siRNA (CCNA2) resulted in a significant accumulation of cells in prophase and mitosis to a similar degree to that observed for cyclin B1 (CCNB1), corresponding with the requirement for cyclin A for G1/S and G2/M transitions. In ...
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