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Mismatch in Biological Technology

New statistical technique improves precision of nanotechnology data

... "The curves should have been symmetric. To address this issue, we developed a new modeling technique that uses the data itself to filter out the mismatch step-by-step using the regression technique." Ideally, researchers would search out and correct the experimental causes of these data errors, but ...

Real Time Genomics to Show SLIM Search(TM) Technology on Windows HPC Server 2008 at Bio-IT World Conference

... to researchers using next generation DNA sequencing technologies. The company is building genomics applications based on its own fast, accurate and mismatch tolerant read mapping and alignment algorithm. Real Time Genomics is a private, San Francisco based company backed by investment from Catamount ...

Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations

... publications. As an alternative offering, Transgenomic's SURVEYOR Nuclease cleaves such heteroduplexes with high specificity at sites of base mismatch or small insertions/deletions. It has a proven track record as a robust and reliable tool in analyzing DNA variations, especially where the mutant ...

Using a grating with a grade, engineers trap a rainbow

... But light is more difficult to control at the nanoscale because of natural limits on its diffraction, or ability to resolve. "There is a mismatch between nanoelectronics and nanophotonics," says Gan. "Because of the diffraction limit of light, optical circuits are now much larger than their ...

Memory Pharmaceuticals Reports First Quarter 2008 Financial Results

... announced that it plans to conduct a clinical study of R3487/MEM 3454 on two biomarkers of schizophrenia, P50 sensory gating and mismatch negativity, in patients with schizophrenia. External costs of the biomarker study, and additional formulation and manufacturing activities ...

Treatment with Paliperidone ER May Be Associated with Significantly Reduced Use of Mental Health Resources

... include the lack of a control group; pre/post design comparing historical data with data collected in the trials could create a bias due to the mismatch in settings; and patients may have had more frequent contact with treating physicians and investigators during the trial period which could favor ...

VaxGen Issues Rebuttal to MedCap Letter

... price following the merger announcement. This drop was and remains very disappointing. VaxGen believes it is largely attributable to a mismatch between the expectations of certain stockholders related to asset value and transaction type, and transactions that were actually ...

NIST demos industrial-grade nanowire device fabrication

... process, the gold deposits bead up into nanodroplets that act as nucleation points for crystals of zinc oxide, a semiconductor. A slight mismatch in the crystal structures of zinc oxide and sapphire induces the semiconductor to grow as a narrow nanowire in one particular direction across the ...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

... Primer sets facilitate study of DNA mismatch repair Mark Dycaico Stratagene ... primer sets from the cDNA sequences of mismatch repair genes. These genes code for specialized ... genes. The primer pairs can be used to detect mismatch repair gene transcripts by the reverse ...

Heteroduplex Analysis Using the DCode System

... of heteroduplex molecules. 1 Heteroduplex molecules have a mismatch in the double-strand, causing a distortion in its usual ... in mobility. Heteroduplex molecules with as little as one mismatch can show a different mobility in a gel from homoduplex ...

The TripleMaster PCR System for three different amplifications

... of the base sequence was determined using the IMBP (Immobilizing mismatch Binding Protein [3]) method following the amplification of a 160 ... [3] Wagner, R. and Dean, A. 1998. The use of immobilizes mismatch binding protein for optimization of PCR fidelity. In: PCR Methods ...

Lessons in Offshore Software Development

... communicating requirements, designing the software, tracking development and making sure the software matches the specs. The goal is to avoid a mismatch between expectations and deliverables. There can be problems with communication and understanding of requirements. It is even more important to ...

Fast Track: Topics for Consideration

... discoveries is creating a tremendous strain on the technical and scientific capabilities of the agency. One reason for this strain is the frequent mismatch between FDA expertise and new product development. Another reason is that FDA reviewers often are isolated from new scientific and technical ...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

... absorbance of Costar wells containing water is approximately .039 to .049 OD. Thus, for samples having raw absorbance values of 1 OD, the worst-case mismatch between plates would still produce an error of 1% or less. However, the lower the absorbance values of the samples, the greater the maximum potential ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... within a model system to determine the feasibility of using DGGE in a clinical environment for the detection of mutations in the mismatch repair genes relevant to the development of Hereditary Non-Polyposis Colon Cancer. We selected exon 4 of the hMSH2 gene as a ...

Designing Controls for siRNA Experiments

... the antisense siRNA strand and the target mRNA affect silencing. Some reports indicate that a single mismatch in the center of an siRNA sequence can abolish silencing effects (1,2). In fact, this remarkable ...

Detecting miRNA & siRNA

... kidney and 32 nt long probes generated with the mir Vana miRNA Probe Construction Kit. mir-16 mut probe (32 nt) carries 3 mismatch mutations (ACG to CGA) corresponding to nucleotides 9 to 11 of the miR-16 miRNA sequence. The mir-16+4 probe (36 nt) carries 4 ...

Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

... we used these products for a Northern blot analysis of MSH2 gene transcription in a hamster cell model system. MSH2 is important for DNA mismatch repair, and mutations in this gene are responsible for many cases of hereditary nonpolyposis colorectal cancer. 1 The MSH2 gene encodes a 3.1-kb ...
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