Melting defects could lead to smaller, more powerful microchips
... by Liquefaction (SPEL), achieves this by melting
the structures on a chip momentarily, and guiding ... straighter, and dots become rounder.
by direct heating has previously been shown to ... semiconductors and metals, which have much higher melting
points. Heating the chip to such temperatures ...
Roche to Introduce New LightCycler(R) MRSA Advanced Test in the EU
... utilized real-time amplification systems available. The LightCycler(R) 2.0 Instrument was the first system to introduce hybridization probes, true melting
curve analysis, automated absolute quantification, and relative quantification with efficiency correction. Combining the power of the LightCycler(R) ...
Venter Institute Launches the J. Robert Beyster and Life Technologies 2009-2010 Research Voyage of the Sorcerer II Expedition
... up the West Coast of the U.S. and into Alaska. While in Alaska the Sorcerer II took samples in Glacier Bay National Park including water taken from melting
Throughout 2008, the Sorcerer II conducted extensive sampling in the waters off San Diego and the coast of California, Oregon ...
Paper electrified by copper particles
... nanoparticles the number of surface atoms is considerably greater than the number of atoms inside the material, which, among other things, makes the melting
temperature of nanomaterials very low. With suitable heat treatment (sintering), the particles manufactured by the research group can be made to form ...
MIT uses nano-origami to build tiny electronic devices
... have developed a few ways to do this successfully: one uses magnets; another involves attaching polymers to a certain spot on the faces and melting
them with an electric current, sealing the two faces together.
They're still working on getting faces and edges of a folded cube to line up with ...
Creating unconventional metals
... that something which was thought to exist theoretically in mathematical physics could actually be found in an alloy which was simply formed by melting
together a few common elements."
Professor Gabriel Aeppli of UCL (University College London), another member of the research team and Director of ...
Principle of Recursive Genome Function Supersedes Dogmas; By Andras Pellionisz, Online Ahead of Print; (Scientific Visionary Vindicated)
... "genome-wide" for more data, as next-generation sequencing made the entire genome of many species (including humans) available with rapidly melting
Application of brute force to turn out more data instead of revising axioms created its own problems, however. A dreaded DNA data ...
Metals shape up with a little help from friends
... absence of air to convert the polymers to a carbon scaffold. The scaffold is then allowed to cool. Because the metal nanoparticles have a very low melting
point, without the carbon scaffold they would stubbornly fuse together in an uncontrolled fashion. Using this process, the carbon scaffold can be ...
In 'novel playground,' metals are formed into porous nanostructures
... step is to heat the material to a higher temperature in air to oxidize the ligands and burn away the carbon. Metal nanoparticles have a very low melting
point at their surface, so the particles sinter together into a solid metal structure. The researchers have made fairly large chunks of porous ...
ARUP Laboratories Partners With Pathology Service Associates
... ARUP's Anatomic Pathology Division
continues to update its technologically-advanced menu by offering a
multitude of molecular techniques, such as melting
curve analysis, PCR, and
fluorescence in situ hybridization (FISH) techniques for detection of gene
amplification involving HER-2/neu, EGFR, and ...
NC State breakthrough results in super-hard nanocrystalline iron that can take the heat
... have developed an iron-zirconium alloy that retains its nanocrystalline structures at temperatures above 1,300 degrees Celsius approaching the melting
point of iron.
Kris Darling, a Ph.D. student at NC State who led the project to develop the material, explains that the alloys ability to retain ...
Femtogram-level chemical measurements now possible, U. of I. team reports
... of the sample can be determined by a cantilever resonance technique.
To analyze the sample, the heater temperature is raised to slightly above the melting
point of the sample material. The material is then analyzed by complementary Raman or Fourier transform infrared spectroscopic imaging, which ...
Practical Applications of HyperDSC in a Pharmaceutical Laboratory
... to detect such events as glass transition or the melting
of a compound when they are concealed by kinetic ... of heat flux up to 500C/min.
peak of an Indium standard has reliable onset and ... which a very weak endotherm was detected for the melting
of Carbamazepine form III, a polymorph making up ...
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
... 10 C higher than
the calculated primer melting
temperature for the first cycle, then decreasing ... difference yields a DNA fragment with lower %G+C, melting
at a lower denaturant
concentration to ... base differences did not change the %G+C of the melting
domain in the
Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
... with increasing
temperature until the melting
temperature of the probe-template hybrid
... point of maximum rate of change is considered the melting
temperature of the probe-template ... a mutation. The
distinctly different melting
temperatures of the two possible hybrids
Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
... change in fluorescence accompanies
of the double-stranded PCR products. A plot of ... in fluorescence as distinct peaks.
temperature (T m ) of each product is defined as ... peak in the no-template control wells at a melting
(T m ) of approximately ...
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
... denaturing gels were run to determine their melting
profiles empirically. The resulting ... in order to construct a theoretical
profile of the PCR product (Figure 1). The ... to obtain very large fragments with ideal melting
properties. One strategy
is to amplify ...
Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
... Table 3.
Figure 2 shows the melting
curves for the four replicate lambda DNA ... point of inflection for the curve,
point (T m ), is clearly seen as a single, common ... 82C. This peak corresponds to the predicted melting
temperature of the
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
The results of the melting
curve analysis are presented in Figures 2
... Plotting the negative first derivative shows the melting
(T m ) as a single peak at ...
the EPSPS primers. Comparison of the melting
curves of the spiked and
nonspiked soy ...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
... and wild-type molecules have different
behavior and thus begin to denature at different ...
Another approach uses the theoretical melting
behavior of the DNA sequence
as a ... this method, the first step is to ascertain the melting
the sequence of interest. ...
TTGE on the DCode System
... a wider range, several fragments with different melting
can be analyzed on the same ... CDGE detects nearly all mutations within one melting
domain, but has the
disadvantage of ... with only
one low and one high melting
domain will often have a short transition
Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
... a gradient of chemical denaturant.
is sequence specific and occurs in discrete segments called
domains. Since melting
involves breaking the hydrogen bonds that
Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
... amplified fragments by the differences in their melting
(Fischer and Lerman 1983). By ... of the sequence variation and the lower melting
on the 3' end of the ... 2 codon insert and the
presence of two melting
domains may cause shifting of some
Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
The calculated melting
profile of the generated PCR fragment shows that
it contains three melting
domains. The putative mutations were in the
domain, which could not be analyzed without a GC ...
DGGE Analysis Using the DCode System, Rev B
... will melt in discrete segments
domains. The melting
temperature (T m ) of these
domains is ... creating branched molecules. Partial melting
DNAs mobility in a ...
A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
... greater GC content resulting in higher
temperatures. The ability of DGGE to detect ... alterations is based on the differential melting
homoduplex DNA ... a region of sequence alteration.
characteristics of a double stranded DNA
HotStarTaq PCR Handbook
templates by modifying the melting
behavior of DNA. This unique reagent
... PCR assays.
Q-Solution changes the melting
behavior of DNA and can be used for
... Simplified formula for estimating melting
temperature (T m ):
T m = ...
QIAGEN Multiplex PCR Handbook
by modifying the melting
behavior of DNA. This unique reagent often
... for multiplex PCR. Q-Solution changes the melting
behavior of DNA and will
often improve a ...
Simplified formula for estimating melting
temperature (Tm): Tm = 2C
Using the TripleMaster PCR System for Robust Amplification of
GC-Rich DNA Templates
base pairs and thereby lowering the melting
temperature of DNA. DMSO has
of PCR reactions, their effects on the melting
temperature of DNA alters
the optimal ... first cycle is set at or a few degrees above the melting
of the primers. The ...
General Notes on Primer Design in PCR*
... things such as the length of the product, its melting
temperature and ultimately the yield. A ... are:
... relationship between annealing temperature and melting
is one of the Black Boxes ...
Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
... The length of the primers;
the composition of ... bp long. The influence of the primer's
temperature on the annealing temperature in PCR ... an influence
on primer annealing, the melting
temperature of the PCR product and product
Mx4000 Multiplex Quantitative PCR System*,PPP
discrimination, plate reads, melting
curves, and quantification of single
... QPCR, qualitative detection,
View Real-Time Plots while ... intensity screen, final
call results, melting
curves, annealing range, and text reports.
Denaturing HPLC (DHPLC)
... from the column earlier than the homoduplexes because of their reduced melting
Optimisation of DHPLC, requires the generation of a melting
profile for each PCR fragment to determine its optimal temperature for ...
Real-Time PCR: General Considerations, Rev A
... structure if possible
Use an annealing temperature above the melting
temperature (T m )
for any template secondary structures
... Design primers with a GC content of 5060%
Maintain a melting
temperature (T m ) between 50 and 65C
Eliminate secondary ...
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
... using Vector NTI
software (InforMax, North Bethesda, MD). The melting
temperatures (T m )
of the primer and probe sequences were ... the 5' end, and the quencher DABCYL linked to the
3' end. The melting
temperatures of the hairpin stems and probe sequences
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
... and fluorescence data were collected at a temperature
above the melting
temperature of nonspecific products (Morrison et al.
... PCR product
was verified both by the presence of a single melting
representing a specific product (vs. a ...
Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
... 55 C to allow heteroduplex formation.
temperature of the 430 bp PCR product was ... which correlated to the
temperature of 6570 C. All further analyses
By calculating the predicted melting
temperature of a segment
of DNA, ...
DGGE Analysis for Non-Invasive Studies of Primate Diet
... produced by
To determine the melting
temperature of PCR products, they
were first analyzed on a ...
species possessed identical sequences (unpublished) in the melting
under examination, and thus differentiation could not be ...
UW-Madison Laser Device to Study Polar Ice Caps
... way to precisely measure change over the vast desert of ice. These measurements of elevation change over time will show whether the ice sheets are melting
or growing as the Earth's climate undergoes natural and human-induced changes.