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Lysate in Biological Technology

MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan

... "Tumor lysate electroloaded mature human DC exhibit enhanced antigen-specific potency compared to lysate co-incubation in clinical manufacturing scale" ... Japan. The presentation is entitled: "Tumor lysate electroloaded mature human DC exhibit enhanced ...

Fungitell(R) CE Marked, Referenced in IDSA and NCCN Guidelines and Added to EORTC-MSG Revised Definitions of Invasive Fungal Disease

... wholly owned subsidiary of Seikagaku BioBusiness Corporation, the Company pioneered the humane use of horseshoe crab blood extract, Limulus Amebocyte lysate (LAL), used in the testing of pharmaceuticals, medical devices and biologics, for the presence of potentially toxic endotoxins and glucans. Our ...

Bruker Announces Availability of New HUPO World Congress Proteomics Posters on Web Site

... of Breast Cancer Sections by MALDI Tissue Imaging Poster T175 Glycoproteomics -- Relative Quantification of Glyco-Proteins from Yeast lysate by Means of ConA Capturing, ICPL Labeling and MALDI-TOF MS Poster T341 -- Full Glycoprotein Characterization by the Combination of High ...

Improving the accuracy and speed of mammalian cell counting

... initial sample (200 μL) is added to the cell lysate and vortexed. This subsequent cell lysate is loaded into a Nucleo- Cassette( New Brunswick ... placed into the NucleoCounter for analysis. The lysate dissolves the Propidium Iodide, which is ...

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

... After a 3 second pause, aspirate 110 μL of lysate and transfer to a 96-well round bottom microplate ... gap, and 10 μL Microparticle Solution to the lysate plate 9. Mix the solution using 10 ... removal of supernatant. Selective removal of lysate was accomplished by blowing air into the wells of ...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... the resuspension of the pellet, mixing of the lysate and neutralization solution. - ... Positive pressure is applied to clear the lysate of cellular debris and to transfer the samples to ... is added using standard pipette tips to the lysate in the BAC Filter Plate followed by applying ...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

... other cellular components. The cellular lysate or tissue homogenate is transferred to a purification tray and the lysate or homogenate is passed through the tray under ... 104-106 cells/well. Recommended maximum lysate volume for the 6700 workstation: 250 μL ...

Aurum Plasmid 96 Kit Tech Note, Rev A

... of the binary plate configuration, in which both lysate filtration and plasmid binding plates ... the vacuum manifold chamber. When lysate filtration was complete, the lysate filtration plate was simply detached ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

... Protein samples included an E. coli protein lysate (catalog #163-2110), purified bovine ... system, a 2 mg/ml E. coli protein lysate was separated with the Pro260 and ... The separation of the E. coli lysate on both systems (Figure 2) illustrates that the ...

Translate More Protein with Higher Biological Activity

... traditional reticulocyte lysate systems Higher ... than other E.coli lysate systems Efficient ... However, E.coli derived lysate systems are capable of producing most ...

Translate High Yields of Active Protein

... than those from other E. coli lysate systems Efficient synthesis of a ... Compared to other E. coli lysate systems, ActivePro generates proteins with ... with those from two other E. coli lysate kits. The experiment includes both a prokaryotic ...

Ready for Real-time in 5 Minutes

... Green qRT-PCR from lysate equivalent to 3 cells ... Cells-to-cDNA II Kit for cell lysate preparation for direct use ... room temperature. The cell lysate can be used directly for ...

Isolate It All: siRNA miRNA Total RNA Native Protein

... lysate. A fraction of the lysate can be used directly to ... RNA isolation, the HeLa cell lysate was immediately mixed with an equal ... affect RNA quality. The advantage is that this lysate contains native protein that can be ...

Increase Protein Activity and Yield

... yields of protein than reticulocyte lysate systems Fully compatible with ... activity than other E. coli lysate systems Easy to use For ... ActivePro uses an E. coli lysate to synthesize high yields of ...

QIAshredder Homogenizer

... Up to 700 l of a cell lysate can be homogenized by pipetting or decanting the lysate into a QIAshredder unit and centrifuging for 2 ... full speed in a microcentrifuge. The homogenized lysate is ready to use in nucleic acid ...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

... chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is ... lysis of the pelleted cells, the lysate is neutralized using an optimized buffer, ... or filtration of the lysate is not required, and chaotropic salts ...

EndoFree Plasmid Kits

... Following clearing of the bacterial lysate using the QIAfilter Cartridge (see " ... Buffer is added to the filtered lysate which is incubated on ice. The lysate is then applied directly to a ...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

... of cellular RNases. The RNA in the lysate is then selectively bound to a ... 1 min. using a pestle. Transfer lysate into a 50 ml centrifuge tube which can withstand ... Add 18 ml 70% ethanol to lysate and completely mix via very gentle, ...

Yeast Protein Production System Features High Yields and One-Step Purification

... in S. pombe. Figure 2 shows the cell lysate samples derived from repressed and induced ... under induced conditions, the crude lysate shows a dominant band at the size expected ... (43 kDa) (figure2, lane 4). In the lysate of cells grown under repressed conditions, this ...

Purified Single-Stranded M13 DNA for Automated Sequencing

... of single-stranded DNA recovered from phage lysate exceeds the yield obtained with the leading ... StrataPrep M13 method of Phagescript lysate (a modified M13 bacteriophage 8 ), was at least ... Recover single-stranded DNA from phage lysate more efficiently using the StrataPrep method than ...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

... system to purify a 75 kD His-tagged protein from crude E. coli lysate on a Profinity IMAC Ni 2+ -charged resin. In the present ... of loading buffer at a flow rate of 1.5 ml/min. Crude E. coli lysate (250 l) containing a 75 kD His-tagged protein was loaded into a ...

Nuclease Protection Assays: The Basics

... Protect lysate RPA Kits) all require the use of RNA probes. ... Direct Protect lysate RPA Kit 100 rxns ...

Translate High Yields of Biologically Active Proteins

... Figure 3. Comparison of Protein Yields Across E. coli lysate Kits. DNA templates for 5 human proteins were in vitro ... 4. Comparison of Biological Activity Across E. coli lysate Kits. The proteins as produced from Figure 3 were tested in ...

Hands-free Tissue Disruption No Homogenization Needed

... the MELT Total RNA Isolation System (Ambion). An aliquot of the lysate was immediately purified and the remaining lysate stored at room temperature (2225C) for 6 days. (A) Agilent ...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

... Kit (Ambion) (final volume = 500 l). 3 l of the cell lysate was used in one-step RT-PCR, using TaqMan Primer & Probe sets ... Kit (Ambion) (final volume = 100 l). 3 l of the cell lysate was used in two-step RT-PCR, using SYBR Green on the ABI Prism ...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

... cleavage site for TEV protease lysate preparation The pellet was lysed using the ... Results The fractions corresponding to the lysate supernatant, glutathione-resin eluate, ... major protein detectable in the supernatant after lysate centrifugation (lane S). The first purification ...

Protocol for GenomePlex Whole Genome Amplification from Blood Card

... Discard the flow-through liquid. Add 900 l of 95-100% ethanol to the lysate in the 15 ml conical tube and mix thoroughly by vortexing 5 to 10 ... at ≥ 6,500 x g for 1 minute. Repeat this until all of the lysate has been passed through the column. Discard the collection tube and ...

A Unique Dimension in Biological Purity

... LAL is the abbreviation for Limulus Amoebocyte Lysate. This lysate of blood cells from Limulus polyphemus (horseshoe crab) reacts ... addition of a solution containing endotoxins to a solution of the lysate produces turbidity, precipitation or gelation of the mixture. ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... by vortexing with acid-washed glass beads. The cleared cell lysate was used to measure luciferase activity. In both cases, strong ... glucose or galactose as the carbon source, and the cleared cell lysate was assayed for luciferase activity. As shown in Figure ...

Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

... Transfection procedure, maintenance of transfected cells, cell lysate preparation, and luciferase assay have been described ... Transfection procedure, maintenance of transfected cells, cell lysate preparation, and luciferase assay are previously described. 5 ...

QuickLyse Miniprep Kit

... is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied to a QuickLyse Spin Column without any additional centrifugation step. Plasmid DNA binds to the column membrane and ...

Research Tools for Analysis of ZAP-70

... surface. A phycoerythrin conjugated detector antibody is also employed. In the case of the BD CBA total ZAP-70 Flex Set, ZAP-70 present in the lysate binds to the capture bead. The amount of PE-anti-ZAP-70 detector antibody that binds to the captured antigen is a measure of the amount of that ...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

... a separate vessel. The culture supernatant was gently aspirated and the cells lyzed with 0.25% (w/v) dodecyltrimethyl ammonium bromide. An aliquot of lysate was transferred to a second plate for assay.

Working standards (0.2–25.6 pmol/well) were pipetted into empty wells of the plate. ...

Addition of imidazole during binding improves purity of histidine-tagged proteins

... To determine the optimal imidazole concentration to use during the binding of (His) 6 -PknG to Ni Sepharose High Performance, a cell lysate containing (His) 6 -PknG was loaded on a column with 10 mM imidazole in the binding buffer. After additional washing with binding buffer, (His) 6 ...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

... columns were also compared under denaturing conditions. Histidine-tagged Maltose Binding Protein (MBP-(His) 6 ; M r 43 000) in E. coli BL-21 lysate containing 7 M urea was applied to the columns. Elution was achieved by lowering the pH from pH 8.0 to pH 4.5. Although denaturing conditions ...

Protein Truncation Tests Using the DCode System

... amplified product was used to produce protein in the coupled transcriptiontranslation reaction of the TNT Coupled Reticulocyte lysate System (Promega). Reactions were performed in 12.5 ml with 6 Ci of 35 S-methionine. Protein products were run on a 16 x 20 cm, ...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

... bacteria (Figure 1). Comparison of gel profiles shows that a large number of proteins are extracted in the E1 cell lysate fraction. Membrane-associated (less soluble) fractions were then obtained following E2 and E3 separation. Subsequent comparison ...

Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays

... dose-response relationship between the concentration of each protein target and the corresponding signal when a specific cell lysate sample is tested. These results demonstrate that multiplexing a total assay with five phosphoprotein assays can detect ...

Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

... and total ERK and p38 MAPK. Methods lysate Preparation Lysates of HEK 293 and HeLa cells were prepared ... (Thr 180 /Tyr 182 ) was detected by ELISA in UV-treated HEK 293 lysate with protein concentration ranging from 12 g/well to 0.75 ...
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