MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan
... "Tumor lysate
electroloaded mature human DC exhibit enhanced
antigen-specific potency compared to lysate
co-incubation in clinical
The presentation is entitled: "Tumor lysate
electroloaded mature human
DC exhibit enhanced ...
Fungitell(R) CE Marked, Referenced in IDSA and NCCN Guidelines and Added to EORTC-MSG Revised Definitions of Invasive Fungal Disease
... wholly owned subsidiary of Seikagaku BioBusiness Corporation,
the Company pioneered the humane use of horseshoe crab blood extract,
Limulus Amebocyte lysate
(LAL), used in the testing of pharmaceuticals,
medical devices and biologics, for the presence of potentially toxic
endotoxins and glucans. Our ...
Bruker Announces Availability of New HUPO World Congress Proteomics Posters on Web Site
... of Breast Cancer Sections by MALDI Tissue Imaging Poster T175
-- Relative Quantification of Glyco-Proteins from Yeast lysate
by Means of ConA Capturing, ICPL Labeling and MALDI-TOF MS Poster T341 -- Full Glycoprotein Characterization by the Combination of High ...
Improving the accuracy and speed of mammalian cell counting
... initial sample (200 μL) is added to the cell lysate
and vortexed. This subsequent cell lysate
is loaded into a Nucleo- Cassette( New Brunswick ... placed into the NucleoCounter for analysis. The lysate
dissolves the Propidium Iodide, which is ...
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation
... After a 3 second pause, aspirate 110 μL of lysate
and transfer to a 96-well round bottom microplate ... gap, and 10 μL Microparticle Solution to the lysate
9. Mix the solution using 10 ... removal of supernatant. Selective removal of lysate
was accomplished by blowing air into the wells of ...
Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
... the resuspension of the pellet, mixing of the lysate
and neutralization solution.
- ... Positive pressure is applied to clear the lysate
of cellular debris and to transfer the samples to ... is added using standard pipette tips to the lysate
in the BAC Filter Plate followed by applying ...
Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
... other cellular components.
The cellular lysate
or tissue homogenate is transferred to a purification tray and the lysate
or homogenate is passed through the tray under ... 104-106 cells/well. Recommended maximum lysate
volume for the 6700 workstation: 250 μL ...
Aurum Plasmid 96 Kit Tech Note, Rev A
... of the binary plate configuration, in which both lysate
plasmid binding plates ... the vacuum manifold chamber. When
filtration was complete, the lysate
filtration plate was simply
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
... Protein samples included an E. coli protein lysate
purified bovine ...
system, a 2 mg/ml E. coli protein lysate
was separated with the Pro260
and ... The separation of the E. coli
on both systems (Figure 2) illustrates that the ...
Translate More Protein with Higher Biological Activity
Higher ... than other
Efficient ... However, E.coli
systems are capable of producing most
Translate High Yields of Active Protein
... than those from other E.
Efficient synthesis of a ... Compared
to other E. coli lysate
systems, ActivePro generates proteins with
... with those from two other E. coli
kits. The experiment includes both a prokaryotic ...
Ready for Real-time in 5 Minutes
qRT-PCR from lysate
equivalent to 3 cells
... Cells-to-cDNA II Kit for cell lysate
preparation for direct
room temperature. The cell lysate
can be used directly for
Isolate It All: siRNA miRNA
Total RNA Native Protein
lysate. A fraction of the lysate
can be used directly to
... RNA isolation,
the HeLa cell lysate
was immediately mixed with an equal
... affect RNA quality. The advantage is that this lysate
contains native protein that can be ...
Increase Protein Activity and Yield
... yields of protein than reticulocyte
Fully compatible with ... activity than other E. coli
Easy to use
For ... ActivePro uses
an E. coli lysate
to synthesize high yields of
Up to 700 l of a cell lysate
can be homogenized by pipetting or
decanting the lysate
into a QIAshredder unit and centrifuging for 2 ... full speed in a microcentrifuge. The homogenized lysate
is ready to use
in nucleic acid ...
DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid
... chemistry eliminates the need
for a lysate
clearing step. Plasmid DNA purification is ... lysis of the
pelleted cells, the lysate
is neutralized using an optimized buffer,
or filtration of the lysate
is not required, and chaotropic salts
EndoFree Plasmid Kits
Following clearing of the bacterial lysate
using the QIAfilter Cartridge
(see " ... Buffer is added to the filtered
which is incubated on ice. The lysate
is then applied directly
to a ...
Simple and Rapid Isolation of Plant RNA
Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
of cellular RNases. The RNA in the lysate
is then selectively bound to
a ... 1 min. using a pestle.
into a 50 ml centrifuge tube which can withstand ...
Add 18 ml 70% ethanol to lysate
and completely mix via very gentle,
Yeast Protein Production System Features High Yields and One-Step
... in S. pombe. Figure
2 shows the cell lysate
samples derived from repressed and induced
... under induced
conditions, the crude lysate
shows a dominant band at the size expected
... (43 kDa) (figure2, lane 4). In the
of cells grown under repressed conditions, this ...
Purified Single-Stranded M13 DNA for Automated Sequencing
... of single-stranded DNA recovered from phage lysate
exceeds the yield
obtained with the leading ... StrataPrep M13 method of Phagescript
(a modified M13 bacteriophage 8 ), was at least ... Recover single-stranded DNA from phage lysate
more efficiently using the StrataPrep method than ...
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
... system to purify a 75 kD His-tagged protein
from crude E. coli lysate
on a Profinity IMAC Ni 2+ -charged
resin. In the present ... of loading
buffer at a flow rate of 1.5 ml/min. Crude E. coli lysate
containing a 75 kD His-tagged protein was loaded into a ...
Nuclease Protection Assays: The Basics
RPA Kits) all require the use of RNA
Direct Protect lysate
Translate High Yields of Biologically Active Proteins
... Figure 3. Comparison of Protein Yields Across
E. coli lysate
Kits. DNA templates for 5 human proteins were
in vitro ... 4. Comparison
of Biological Activity Across E. coli lysate
proteins as produced from Figure 3 were tested in ...
Hands-free Tissue Disruption No Homogenization Needed
... the MELT Total RNA Isolation System (Ambion).
An aliquot of the lysate
was immediately purified and the remaining lysate
stored at room temperature (2225C) for 6 days. (A) Agilent ...
Fast, Accurate Assessment of siRNA-induced Gene Silencing
... Kit (Ambion) (final
volume = 500 l). 3 l of the cell lysate
was used in one-step RT-PCR,
using TaqMan Primer & Probe sets ... Kit (Ambion) (final volume = 100 l). 3 l of the
was used in two-step RT-PCR, using SYBR Green on the ABI Prism
Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
... cleavage site for TEV protease
The pellet was lysed using the ... Results
The fractions corresponding to the lysate
supernatant, glutathione-resin eluate, ... major protein detectable in the supernatant after lysate
centrifugation (lane S). The first purification ...
Protocol for GenomePlex Whole Genome Amplification from Blood Card
... Discard the flow-through liquid.
Add 900 l of 95-100% ethanol to the lysate
in the 15 ml conical tube and mix thoroughly by vortexing 5 to 10 ... at ≥ 6,500 x g for 1 minute. Repeat this until all of the lysate
has been passed through the column.
Discard the collection tube and ...
A Unique Dimension in Biological Purity
... LAL is the abbreviation for Limulus Amoebocyte
Lysate. This lysate
of blood cells from Limulus polyphemus (horseshoe
crab) reacts ... addition of a solution containing endotoxins to a solution
of the lysate
produces turbidity, precipitation or gelation of the mixture.
Epitope-Tagging Vectors for Functional Analysis in Yeast
... by vortexing with acid-washed glass beads. The cleared cell
was used to measure luciferase activity. In both cases, strong
... glucose or galactose as
the carbon source, and the cleared cell lysate
was assayed for luciferase
activity. As shown in Figure
Assess the In Vivo Activation of Signal Transduction Pathways with
PathDetect Reporting Systems
... Transfection procedure,
maintenance of transfected cells, cell lysate
preparation, and luciferase
assay have been described ... Transfection procedure, maintenance
of transfected cells, cell lysate
preparation, and luciferase assay are
previously described. 5 ...
QuickLyse Miniprep Kit
... is bound in a single
buffer, enabling fast DNA purification with minimal hands-on time. After
a 3-minute lysis step, the clear lysate
is applied to a QuickLyse Spin
Column without any additional centrifugation step. Plasmid DNA binds to
the column membrane and ...
Research Tools for Analysis of ZAP-70
... surface. A phycoerythrin conjugated detector antibody is also employed. In the case of the BD CBA total ZAP-70 Flex Set, ZAP-70 present in the lysate
binds to the capture bead. The amount of PE-anti-ZAP-70 detector antibody that binds to the captured antigen is a measure of the amount of that ...
Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
... a separate vessel. The culture supernatant was gently aspirated and the cells lyzed with 0.25% (w/v) dodecyltrimethyl ammonium bromide. An aliquot of lysate
was transferred to a second plate for assay.
Working standards (0.2–25.6 pmol/well) were pipetted into empty wells of the plate. ...
Addition of imidazole during binding improves purity of histidine-tagged proteins
To determine the optimal imidazole concentration to use during the binding of (His) 6 -PknG to Ni Sepharose High Performance, a cell lysate
containing (His) 6 -PknG was loaded on a column with 10 mM imidazole in the binding buffer. After additional washing with binding buffer, (His) 6 ...
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
... columns were also compared under denaturing conditions. Histidine-tagged Maltose Binding Protein (MBP-(His) 6 ; M r 43 000) in E. coli BL-21 lysate
containing 7 M urea was applied to the columns. Elution was achieved by lowering the pH from pH 8.0 to pH 4.5. Although denaturing conditions ...
Protein Truncation Tests Using the DCode System
... amplified product was used
to produce protein in the coupled transcriptiontranslation reaction of
the TNT Coupled Reticulocyte lysate
System (Promega). Reactions were performed
in 12.5 ml with 6 Ci of 35 S-methionine. Protein products were run on
a 16 x 20 cm, ...
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
... bacteria (Figure 1).
Comparison of gel profiles shows that a large number of proteins are
extracted in the E1 cell lysate
fraction. Membrane-associated (less soluble)
fractions were then obtained following E2 and E3 separation. Subsequent
Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
... dose-response relationship
between the concentration of each protein target and the corresponding
signal when a specific cell lysate
sample is tested. These results demonstrate
that multiplexing a total assay with five phosphoprotein assays can detect
Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
... and total ERK and p38 MAPK.
Lysates of HEK 293 and HeLa cells were prepared ... (Thr 180 /Tyr 182 ) was detected by ELISA in UV-treated HEK
with protein concentration ranging from 12 g/well to 0.75