DNA is blueprint, contractor and construction worker for new structures
... single-stranded portion of this DNA serves as the linker
DNA, which seeks out a complementary single ... The binding of the two single strands of linker
DNA to each other completes the double helix, ... structure -- a crystal. One sequence of linker
DNA, programmed by the researchers, results in ...
Medarex Announces Allowance of Investigational New Drug Application for Company's First Antibody-Drug Conjugate, MDX-1203
... linker. Medarex believes that its ADC technology overcomes many of the key development challenges of drug conjugates, including issues of linker
stability, potency and multi-drug resistance while maintaining a wide therapeutic window with minimal toxicity.
Medarex has conducted multiple ...
Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... for whole genome analysis under
development by Transgenomic. It identifies DNA variations between a reference
genome and a test genome. Using a linker
that attaches an identification tag
of known sequence to the DNA ends processed by the SURVEYOR Nuclease, SEAL
generates a library of genetic ...
Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... for whole genome
analysis under development by Transgenomic. It identifies DNA variations
between a reference genome and a test genome. Using a linker
an identification tag of known sequence to the ends cleaved by the SURVEYOR
Nuclease, SEAL generates a library of genetic variations ...
Taxol bristle ball: a wrench in the works for cancer
... they needed to perform to create the ball. Using the wrapped version of the drug, they undertook a series of reactions to attach the drug to linker
molecules that were, in turn, attached to the ball. In the final step of the reaction, they dissolved the wrapper, restoring the key.
"We are ...
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the
GFP linked by a 27 amino acid peptide linker
that contains a recognition site
for ... 25C
to promote cleavage of the peptide linker
by trypsin (Figure 2). Using the Scan
... addition of trypsin, which cleaves the peptide linker
that tethers the GFPs. A small ...
Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
... sterilization method using the
GS Gene linker
UV chamber to destroy contaminating amplicon or ... placed in the middle of the GS Gene
oven and were either capped and placed on their ... UV light was delivered
by the GS Gene linker
chamber for 30 minutes. After UV irradiation was ...
UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
... to HCl nicking.
The GS Gene linker
UV chamber contains preprogrammed routines for ... with 60 mJoules of energy in the GS Gene linker
after staining with ... on a glass tray for transportation to the GS Gene linker
chamber, place the tray inside the GS ...
Synthesize High Yields of Biotinylated aRNA
... single biotin UTP label.
While various linker
arm sizes (e.g.,
Biotin-11-UTP, Biotin-16-UTP) did ... UTP
incorporation into aRNA during IVT, the longer linker
slightly impeded subsequent purification of the ...
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)
short lengths of nucleic acid stretches, ~1kb or less. Four WGA methods have been
described and are in common use they include linker
adapter PCR (LAP) 8-10 , primer
extension preamplification PCR (PEP) 11 , degenerate oligonucleotide PCR (DOP) 12,13 and
multiple strand ...
Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
Our results show that a protein construct expressed as a GST-HIS fusion with a protease recognition site as a linker
is suitable for being quickly purified using two affinity columns. This protocol provides the possibility to eliminate contaminant native proteins ...
Efficient and Reliable Linear
Amplification of cRNA
... T7 RNA polymerase.
For first-strand cDNA synthesis an oligo(dT) primer
linked to the T7 promoter sequence is used. In the next
step, this T7 linker
allows the linear amplification of the template and the simultaneous incorporation of labeled
nucleotides with T7 RNA polymerase, generating ...
Quantification of Nucleosomes in Serum
by the Cell Death Detection ELISAplus
elementary units of chromatin formed by an octamer of
histones and 146 bp DNA wrapped around it. The nucleosomes
are connected by linker
DNA of about 15 -100bp
which are the preferential binding sites of the endonucleases
[9-11]. Under physiological conditions, nucleosomes
The DIG System Nonradioactive and
Highly Sensitive Detection of Nucleic Acids
Well-established protocols (many years
Easy stripping and reprobing of Southern blots
(alkali-labile DIG linker
for DNA probes)
Special protocol for easy stripping of
The DIG System allows the safe and ...
An Optimal DNA Microarray Substrate for the Identification of Fetal
and Somatic Liver Stem Cells of the Rat
... range of measurable signals is very high.
Binding the probes (in this case: 50mer oligonucleotides) terminally,
modified with a linker
molecule (5'aminoalkane molecule), to the slide
surface results in the advantage that the entire length of spotted oligonucleotides