World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc.
... in the US, Europe, and Japan. Some of the alternative technologies existing in the market include OmniPlex, Strand Displacement Amplification (SDA), ligase
Chain Reaction (LCR), and Hybrid Capture technology.
Gene amplification technology plays a vital role in the diagnostics field. With increasing ...
High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures
... reactions consisted
of DNA, 1x ligase
buffer (50 mM Tris, pH 7.6, 10 mM MgCl 2 , 10 mM ... and 25 g/ml BSA), and 0.1 Weiss unit/l T4 DNA ligase
All ligations reactions were incubated at 16 C overnight. The ligase
was heattreated by placing the ...
Eppendorf Perfectprep Gel Cleanup Kit
... Alkaline Phosphatase (Promega )
T4 DNA ligase
(New England BioLabs)
TOP 10 Chemically ... end units (about 6 Weiss units) of T4 DNA ligase
and 5% PEG 8000 were
incubated at 14C overnight (15 hours). Following this, the ligase
was inactivated by incubating the ...
Mammalian Expression Vector for Efficient Cloning of PCR Fragments
... insert and vector in the
presence of T4 DNA ligase
and Srf I enzyme reduces the background ... of the Srf I enzyme and the T4 DNA ligase
In many ... 3 Srf I restriction enzyme, T4 DNA ligase
Epicurian Coli XL10-Gold ultracompetent ...
Get the 5'-End
... Liu, X. and Gorovsky, M.A.1993. Mapping the
5' and 3' ends of Tetrahymena thermophila mRNAs using RNA
mediated amplification of cDNA ends (RLM-RACE). Nucl. Acid Res. 21 :4954-4960
Cloning Based on Efficient Three-Fragment Assembly
we started the reaction at 26C, which was reported
to be the optimal temperature for the joining of bluntended
termini by T4 DNA ligase
. We followed this
with cohesive-end ligation and circularization of our
DNA at 14C, the temperature generally regarded as
the best ...
Constructing Directional cDNA Libraries from Limited Amounts of RNA
... be used for high-throughput 3 EST sequencing
and for functional screening. Additionally, this method does not depend on the
use of T4 DNA ligase
to covalently link the vector and insert; consequently, the
background of self-ligated vector is reduced. Since PCR products are cloned
Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal
... of enzyme, and the enzyme is removed using StrataClean
resin. The digestion products are ligated using Stratagenes T4 DNA
and then recut with the same restriction enzyme. Samples of the
cut, ligated, and recut DNA are subsequently analyzed by agarose gel ...
Enhanced PCR Cloning System
... vector and increased ligation efficiency for
blunt-ended DNA fragments by the simultaneous, opposing reactivity of the Srf
I enzyme and T4 DNA ligase
on nonrecombinant vector DNA. Ligated DNA is then
ready to be transformed into competent cells.
The PCR-Script kits, although ideal to use with ...