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Ligase in Biological Technology

World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc.

... in the US, Europe, and Japan. Some of the alternative technologies existing in the market include OmniPlex, Strand Displacement Amplification (SDA), ligase Chain Reaction (LCR), and Hybrid Capture technology. Gene amplification technology plays a vital role in the diagnostics field. With increasing ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

... reactions consisted of DNA, 1x ligase buffer (50 mM Tris, pH 7.6, 10 mM MgCl 2 , 10 mM ... and 25 g/ml BSA), and 0.1 Weiss unit/l T4 DNA ligase (Bio-Rad). All ligations reactions were incubated at 16 C overnight. The ligase was heattreated by placing the ...

Eppendorf Perfectprep Gel Cleanup Kit

... Alkaline Phosphatase (Promega ) T4 DNA ligase (New England BioLabs) TOP 10 Chemically ... end units (about 6 Weiss units) of T4 DNA ligase and 5% PEG 8000 were incubated at 14C overnight (15 hours). Following this, the ligase was inactivated by incubating the ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... insert and vector in the presence of T4 DNA ligase and Srf I enzyme reduces the background ... of the Srf I enzyme and the T4 DNA ligase on nonrecombinant vector DNA. In many ... 3 Srf I restriction enzyme, T4 DNA ligase and Epicurian Coli XL10-Gold ultracompetent ...

Get the 5'-End

... Liu, X. and Gorovsky, M.A.1993. Mapping the 5' and 3' ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucl. Acid Res. 21 :4954-4960 Who ...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... Therefore, we started the reaction at 26C, which was reported to be the optimal temperature for the joining of bluntended termini by T4 DNA ligase [7]. We followed this with cohesive-end ligation and circularization of our DNA at 14C, the temperature generally regarded as the best ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... be used for high-throughput 3 EST sequencing and for functional screening. Additionally, this method does not depend on the use of T4 DNA ligase to covalently link the vector and insert; consequently, the background of self-ligated vector is reduced. Since PCR products are cloned without ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... of enzyme, and the enzyme is removed using StrataClean resin. The digestion products are ligated using Stratagenes T4 DNA ligase and then recut with the same restriction enzyme. Samples of the cut, ligated, and recut DNA are subsequently analyzed by agarose gel ...

Enhanced PCR Cloning System

... vector and increased ligation efficiency for blunt-ended DNA fragments by the simultaneous, opposing reactivity of the Srf I enzyme and T4 DNA ligase on nonrecombinant vector DNA. Ligated DNA is then ready to be transformed into competent cells. The PCR-Script kits, although ideal to use with ...
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