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Incubate in Biological Technology

San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator

... like Leap Wireless," said Cathy Pucher, executive director of EvoNexus. "It is this kind of contribution that will enable the start-ups we incubate to become viable companies that contribute jobs and economic growth to the San Diego economy." Entrepreneurs who are interested in being a ...

INVO Bioscience Completes Reverse Merger

... eggs (oocytes) are combined with sperm in the INVOcell, a small biological capsule, which is then sealed and placed in the woman's vaginal cavity to incubate for three days. When the INVOcell is removed, any developing embryos are transferred directly into the woman's uterine cavity, where implantation and ...

Michigan Innovation Equipment Depot Launches Next Phase of Distribution

... equipment that's critical for research is a catalyst for moving companies forward in their life cycle. MIED directly supports Michigan's ability to incubate and grow businesses, and ultimately, the state's new economy." MIED's phase four will make available 350 pieces of technical equipment previously ...

CellCyte Genetics Corp. Forms Device Division to Develop and Manufacture Stem Cell Cultivation and Replication Products

... Berninger, Chief Scientific Officer of CellCyte Genetics explains, "CellCyte's patented biological cell maintenance system has the unique ability to incubate cultured cells with a very efficient and regulatable oxygen supply, making it an ideal choice for culturing cells that are otherwise difficult to ...

DELFIA Protein Kinase assays.

... with a GST tag. 1. In the assay, first incubate the substrate with enzyme (receptor), ATP and ... the phosphorylated products on coated plates. incubate 1-2 hours and wash the plate two or three times ... DELFIA Assay Buffer (200 μl, 250 ng/ml) and incubate for 1-2 h. If performed in the indirect way, the ...

Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry

... tip (MeCN, 50% MeCN, 0.1% TFA) incubate ZipTip pipette tip with Affinity Agent, e.g. Ab Wash with incubation buffer incubate ZipTip pipette tip with blocking agent, e.g. BSA Wash with incubation buffer incubate with sample, e.g., antigen Wash with incubation ...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... 5 µl ED Reagent to all wells and incubate the assay 3 h at room temperature.

1.10 ... and 15 µl CL Substrate to all wells and incubate the assay 1 h at room temperature.

1.11 ... µl p38/EA Reagent mix to all the wells and incubate the assay 30 min at room temperature.

Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

... plate at 7000 cells/well (100 ml aliquots) and incubate at 37 °C overnight in 5% CO 2 together with ... preferable to add the test substance on Day 2 and incubate for a shorter length of time.

4. incubate plate for 1 h at 37 °C in 5% CO 2 . ...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

... a density of ~ 16 000 cells/well.

3. incubate ~ 48 h at 37 °C with 5% CO 2 .

5. incubate at room temperature for 15 min.

6. Add 50 µl assay medium containing agonist and incubate at 37 °C for 30 min.

7. Image ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

... 10–15 000 cells/well.

3. incubate overnight at 37 °C with 5% CO 2 . ... and 2.5 µ M Hoechst.

5. incubate at room temperature for 15 min.

6. ... or Krebs-Ringer buffer containing agonist and incubate at 37 °C for 30 to 40 min as appropriate (see ...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

... µl of culture medium.

3. incubate ~72 h in a humidified environment at 37 ºC ... assay concentration of 100 nM.

8. incubate at room temperature for 10 min.

incubate at 37 ºC for 30 min.

10. ...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

... 96-well plates at 5000 cells/100 μl/well and incubate under standard tissue culture conditions for 24 ... mitomycin C at 100-ng/ml final concentration) and incubate cells for 24 h.

3. Prepare a ... tissue culture medium and add 10-μl/well. incubate cells for 24 h.

4. Remove media, ...

Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System

... of AMARA antibody reagent.

2. incubate plate in darkness for 60 min at room temperature ... of ED/CLS to each well.

4. incubate plate in darkness for 60 min (± 15 min) at ... of EA reagent to each well.

6. incubate plate in darkness at room temperature for at ...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

... using a microplate mixer. 5. incubate 5 min at room temperature. 6. Measure ... Vortex for about 5 sec. 10. Let incubate for 5 min at room temperature. 11. ... 5. Vortex for about 5 sec. 6. incubate at room temperature for 15 min. 7. ...

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

... cultured cells, or bacteria: Just incubate sample with InstaGene matrix at 56C for 1530 min, ... inverting the tube several times. 2. incubate the tube at room temperature for 1530 min. ... Add 200 l of InstaGene matrix to the pellet and incubate at 56C for 1530 min. Note: ...

Genomic DNA Isolation From 510 mg Marine Invertebrate Tissue

... sample back on ice until next step. 3. incubate lysate at 65C for 1560 min. Alternatively, if ... lysate. Mix by inverting capped tube 25 times and incubate at 55C for 3 hr to overnight, until ... sample by inverting the capped tube 25 times and incubate at 37C for 1560 min. ...

Genomic DNA Isolation Protocol for 40 l Avian Whole Blood

... cell clumps are visible after mixing, incubate at 37C until the solution is homogeneous. Samples ... tube and inverting it 25 times, then incubate at 37C for 1560 min. Protein ... or is not tight, repeat step 3, incubate on ice for 5 min, then repeat step 4. ...

DNA Isolation From 50 to 20 mg of Paraffin-Embedded Tissue

... ml capped tube. Add 300 l xylene and incubate 5 min with constant mixing at room ... 4. Add 300 l of 100% ethanol to the tube and incubate 5 min with constant mixing at room ... with a microfuge-tube pestle. 2. incubate lysate at 65C for 1560 min. 3. If ...

DNA Isolation From 300 to 450 mg Dried or 600 to 1,200 mg Fresh Leaf Tissue

... 3050 strokes with a tube pestle. 3. incubate cell lysate at 65C for 60 min. After 30 and 60 ... sample by inverting the capped tube 25 times and incubate at 37C for 15 min. Protein ... content, it may be necessary to incubate sample on ice for 515 min. 4. ...

Setting up a kinase profiler with IMAP

... 3 to the appropriate wells. Step 4. incubate as needed at room temperature to allow ... Cover the plate and protect from light. incubate at room temperature for 3060 minutes. ... Plate incubation incubate the plate in the dark overnight (16-24 ...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

... cells/well in 25 L of U2OS media. incubate overnight at 37C, 5% CO 2. ... to each well. Step 4. incubate at 37C, 5% CO 2 for 4045 minutes ... cells/well in 23 L of U2OS media. incubate overnight at 37C, 5% CO 2 . ...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

... Step 2. incubate the microplate at 37C for the desired ... room on the microplate). Step 3. incubate the microplate at room temperature for 2-5 ... as the control wells without cells. incubate at room temperature for one hour. ...

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

... 8. Repeat steps 5-7 one more time. 9. incubate the open microcentrifuge tube at 37 C for 10-15 ... 11. Add 20 l of proteinase K, mix by vortexing. incubate at 55 C (overnight) or until the ... add 20 l of RNase A solution and incubate at room temperature for 2 minutes. ...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

... 125 l paraformaldehyde (16 %-solution) and incubate 10 minutes on ice. Add 75 l Triton (1 %-solution in distilled water) and incubate 10 minutes on ice. 6. Wash cells in 2 ... 15 g/ml (1.5 l and 100 l staining buffer) and incubate for 30 minutes at RT. For more cells (up to 5 ...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

... 500 l of cell suspension (5 x 105 cells). incubate for 15 minutes at room temperature (RT). ... per 500 l of cell suspension (5 x 105 cells). incubate in the dark for 10 minutes at RT. 7. ... l paraformaldehyde 16 % while stirring. incubate for 10 minutes at RT and store the cells at 4 ...

Uses and Applications of FuGENE 6 Transfection Reagent

... to ensure adequate mixing of the components. incubate at room temperature for 5 minutes. ... tapping or vortexing the tube for 1 second. incubate for at least 15 minutes at room temperature. ... 6 Reagent to 970 l serum-free medium. Mix, then incubate 5 minutes. Add 10 g DNA to the diluted ...

Transfection Reagent Selector Kit Handbook

... incubate cells at 37C and 5% CO 2 in an incubator. The ... Mix mastermix tubes AC by vortexing for 1 second. incubate at room temperature ... Mix mastermix tubes DF by vortexing for 1 second. incubate at room temperature (2025C) for 25 ...

Staphylococcus epidermidis

... by gently mixing with pipette several times. incubate at room temperature for 30 minutes. ... cells and transfer to sterile tube. incubate 90 minutes at 37 C with moderate shaking. Plate on selective BM plates. incubate 20 hours at 37 C. ...

Streptococcus salivarius

... a final concentration of 10% and incubate again for 1 h at 37 C. ... Add 1 ml ice-cold outgrowth medium and incubate for 3 h at 37 C. Plate aliquots onto selective agar plates; incubate 2-3 days at 37 C. ...

Staphylococcus aureus

... by gently mixing with pipette several times. incubate at room temperature for 30 minutes. ... cells and transfer to sterile tube. incubate 90 minutes at 37 C with moderate shaking. Plate on selective BM plates. incubate 20 hours at 37 C. ...

Propionibacterium freudenreichii

... 1:50 in fresh SLB medium and incubate again for about 20 h. ... Immediately add 900 l cold outgrowth medium and incubate for 2.5-3 h at 30 C. Plate cells onto selective SLB plates; incubate 5-7 days at 30 C under anaerobic ...

Pediococcus spp.

... with 0.5 M sorbitol) to 800 ml growth medium. incubate for 2 to 4 hours at 37 C to a cell ... and keep on ice for approx. 5 min. incubate for 2 h at 37 C. Plate aliquots onto selective agar plates; incubate 2-5 days at 37 C. ...

Kluyveromyces lactis

... Resuspend cells in 5 ml washing solution and incubate for 5 min. ... in 0.25 ml of the same solution. incubate for 1 hour on ice. ... Add 1 ml YPD medium with 1 M sorbitol, incubate for 1 hour at 30 C. Centrifuge cells ...

Bifidobacterium animalis

... buffer, dispense in tubes and incubate for 3.5 hours at 4 C. ... Dilute with 800 l outgrowth medium and incubate for 2.5 h at 37 C. Plate onto selective MRS agar plates; incubate anaerobically for 2-3 days at 37 C. ...

Streptococcus salivarius

... a final concentration of 10% and incubate again for 1 h at 37 C. ... Add 1 ml ice-cold outgrowth medium and incubate for 3 h at 37 C. Plate aliquots onto selective agar plates; incubate 2-3 days at 37 C. ...

Staphylococcus aureus

... by gently mixing with pipette several times. incubate at room temperature for 30 minutes. ... cells and transfer to sterile tube. incubate 90 minutes at 37 C with moderate shaking. Plate on selective BM plates. incubate 20 hours at 37 C. ...

Staphylococcus epidermidis

... by gently mixing with pipette several times. incubate at room temperature for 30 minutes. ... cells and transfer to sterile tube. incubate 90 minutes at 37 C with moderate shaking. Plate on selective BM plates. incubate 20 hours at 37 C. ...

Pediococcus spp.

... with 0.5 M sorbitol) to 800 ml growth medium. incubate for 2 to 4 hours at 37 C to a cell ... and keep on ice for approx. 5 min. incubate for 2 h at 37 C. Plate aliquots onto selective agar plates; incubate 2-5 days at 37 C. ...

Propionibacterium freudenreichii

... 1:50 in fresh SLB medium and incubate again for about 20 h. ... Immediately add 900 l cold outgrowth medium and incubate for 2.5-3 h at 30 C. Plate cells onto selective SLB plates; incubate 5-7 days at 30 C under anaerobic ...
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