Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM)
... Prestige Antibody is also associated with over 500 tissue immunohistochemistry (IHC) images, over 100 cell/line immunocytochemistry (ICC) images, immunofluorescence
(IF) and western blotting (WB) data, all publicly accessible via the Human Protein Atlas ( www.proteinatlas.org ).
In August 2008, ...
Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C
... about protein
expression profiles and immunofluorescence
patterns that may be associated
with ... is
indicated with small arrows, showed immunofluorescence
patterns that were
clearly different ...
we show in Figures 4A, D, G, J, and M immunofluorescence
pictures of cryostat
sections from ...
Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
... 48 hr post-transfection
microscopy (Figure 1A). The results clearly ... were analyzed for c-myc protein expression using immunofluorescence
as described in Materials and Methods. ... in only 2 or
3 days. Supporting immunofluorescence
data showed that the siRNA was
Drive Long-Term Silencing with CMV Vectors
... to non-transfected HeLa cells. Panel B shows immunofluorescence
the relative ...
clones using either real-time PCR or immunofluorescence
Clones that showed low ... amount
of GAPDH knocked down. immunofluorescence
was performed using gene specific
Dicer vs. RNase III for Preparation of siRNA Cocktails
GAPDH protein levels were monitored by immunofluorescence
48 hours after
transfection ( B ) and ... the reduction in protein levels was examined by immunofluorescence
microscopy. Fluorescent signal was ... RNase III generated siRNAs to GAPDH.
analysis of GAPDH, La, c-MYC, Cdk-2, Ku-90, and ...
Inducing RNAi with siRNA Cocktails Generated by RNase III
... population was transfected into 293 cells. immunofluorescence
to examine the gene silencing ... at 48
hours post transfection and immunofluorescence
was performed with the
levels. Figure 2B shows representative immunofluorescence
reduction in ...
GENE SILENCING WITH PCR PRODUCTS
... assessed using nuclear staining with DAPI and immunofluorescence
using a c-FOS antibody detected with a ... using nuclear staining with DAPI and
using a GAPDH-specific antibody. The DAPI and immunofluorescence
images are overlaid in the figures. ...
Five Ways to Produce siRNAs
... protocol, at
a final siRNA concentration of 75 nM. immunofluorescence
performed 96 hr post transfection using mouse ... the cells were assessed using nuclear staining with
DAPI and immunofluorescence
using a c-FOS antibody. Non-transfected cells
(NT) as well as ...
Control Your siRNA Research
... Each of these primary antibodies
has been used in immunofluorescence
experiments at Ambion to detect the
reduction in protein levels ... in solution in a 100 g
unit size and are validated for use in immunofluorescence
Note that the concentration and volume will vary ...
Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
NeoFX Transfection Agent (Ambion). immunofluorescence
were used to measure the two putative oncogene targets ... introduced into cells that
express high levels of the miRNA. immunofluorescence
48 hours after transfection
revealed an approximately two-fold ...
Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN
siRNA and TransMessenger Transfection Reagent*
Cells were stained and analyzed after transfection. Double immunofluorescence
labeling and image analysis was performed as described previously ... was. Cells were targeted with siRNA 58 days after
plating. Double immunofluorescence
labeling of MAP2 and the unrelated protein
actin was performed 48 ...
Efficient DNA transfection of primary CNS neurons using TransMessenger
... were fixed using 4% paraformaldehyde 24 hours
after transfection. immunofluorescence
was performed according to standard
procedures using primary ... cultures display
the same GFAP- and MAP2-staining pattern. The immunofluorescence
show that primary cultures of cortical neurons generated ...
Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
... ligand binding4, apoptosis5, immunofluorescence5, and bead-based fluorescent immunoassays (FLISAs) 3.
In the experiments discussed here, immunofluorescence
and FLISA assays were combined in a single well, and the secreted cytokines and cell surface expression of an inducible marker were quantitated ...
Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
... control protein, Pab 1, was not affected (Figure 1A). The specific silencing
of hrp48 and U2AF 50 proteins was also confirmed by immunofluorescence
microscopy (data not shown). Direct observation of the Cy3 fluorescence
demonstrated that both hrp48 and U2AF 50 labeled dsRNA ...
Using Validated siRNAs in Functional Genomic Assays
... they were transfected and transferred to wells
of a 96 well plate. Following overnight recovery, the cells were processed
staining of NF-kappaB.
There are several distinct signaling pathways
that can trigger the nuclear translocation of NF-kappaB. ...
The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
Inhibitors (Ambion) or a negative control miRNA inhibitor (CD). Three
days post-transfection, RAS expression was monitored by immunofluorescence
(A, C) and quantified using MetaMorph software (Universal Imaging corporation)
(B, D; triplicate assays). Modified with ...
Superior Gene Silencing Using Adenoviral Vectors
... to the wild type construct, whereas that of
Ad-C10i was complementary to the mutant construct. (A) Green: GFP.
of the polyQ construct detected with 1C2 antibody.
Ad-G10i reduced wt ataxin-3 levels 95% but did not affect mutant ataxin-3
RNAi: A How To for New Users
... its effects can be
easily monitored by qRT-PCR or other methods at the mRNA
level, or by Western blot or immunofluorescence
at the protein
level. In fact, Ambion provides a highly specific antibody for
measuring these protein level ...
RNAi in Non-Mammalian Cultured Cells
... Pab 1, was not affected (Figure
3A). The specific silencing of hrp48 and U2AF 50 proteins
was also confirmed by immunofluorescence
microscopy (data not
shown). Direct observation of the Cy3 fluorescence demonstrated that
both hrp48 and U2AF 50 ...
More siRNA Vectors for RNA Interference
... than the mRNA-specific
probes. (B) GAPDH protein levels in cells transfected with p Silencer
2.0-U6-GAPDH were analyzed by immunofluorescence
using a GAPDH-specific
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success... reduced survivin mRNA levels in these cells by 80% compared to cells
treated with the negative control siRNA (Figure 5). In addition, immunofluorescence
analysis of survivin protein levels indicated that protein levels were
reduced 76% compared to cells transfected with the ...
Delivering siRNA Using Adenoviral Vectors...
or a scrambled negative control siRNA. The media was changed 4 hours after
infection and cells were analyzed after 72 hours. immunofluorescence was
performed using mouse anti-GAPDH antibody. Green: GAPDH protein. Blue:
DAPI stained nuclei.
Choose the Right Transfection Agent for Your RNAi Experiment... Kit. After transfection of the
positive control, reduction in GAPDH activity can be monitored
by immunofluorescence or Western blot with Ambion's monoclonal
antibody to GAPDH, or by Northern blot, RT-PCR or RPA.
Avoid siRNA Design Altogether!...
synthesized siRNA known to efficiently silence GAPDH, was transfected
into HeLa cells. GAPDH protein levels were analyzed by immunofluorescence
48 hrs later.
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Ordering Information ...
Assessing Gene Function with siRNA Libraries... phenotypes were
then examined by microscopic readout to determine their root
causes. This was done by immunofluorescence staining of
multiple markers, including tubulin to reveal microtubule
distribution. This approach revealed ...
Sensitive detection of tumor cells in
peripheral blood of carcinoma patients
by a reverse transcription PCR method... diagnostic approach is
designed to optimize enrichment
and analysis of tumor cells. The
specificity of the method is shown
by immunofluorescence staining
(figures 1 and 2) and the lower
detection limit by RT-PCR (figure 3).
Figure 1 shows ...
Detection of mRNAs on Cryosections of the
Cardiovascular System Using DIG-Labeled RNA Probes... were briefly rinsed in
distilled water, then counterstained with methylene green.
The sections were mounted with Kaisers glycerin-gelatin.
If immunofluorescence protocols are used in combination
with in situ hybridization, we recommend development
with the ELF substrate (ELF Kit, Molecular Probes).
Effectene Transfection Reagent provides efficient gene delivery
to primary neuronal cell cultures... in triplicate.
Control cells were exposed to Effectene Reagent complexed with plasmid DNA
lacking a CMV eukaryotic promoter. immunofluorescence was performed as previously
Efficient Transfection Using Effectene Reagent